Investigation of bioaccumulation profile of oestrogens in zebrafish liver by hollow fibre protected liquid phase microextraction with gas chromatography-mass spectrometric detection.

The applicability of hollow fibre protected liquid phase microextraction (HF-LPME) for the determination of three oestrogens, namely estrone (E1), 17β-estradiol (E2) and 17α-ethinylestradiol (EE2) from individual zebrafish liver samples, in a bioaccumulation study on these organisms, is reported. The oestrogens were extracted from single, mechanically crushed and minced livers from fish that were heaved in tubes containing water spiked at low concentration of the analytes. Extraction was performed with ∼3 μL of toluene contained in the hollow fibre.