A cytologic aid in the diagnosis of congenital glaucoma.

The superficial corneal epithelium of 17 patients with glaucoma varying in age from nine days to 16 years and 25 normal individuals varying from nine months to 16 years were studied comparing findings using the Giemsa and Papanicolaou staining methods. The normal individuals exhibited a high frequency of precornified cells and a paucity of cornified cells with pyknotic nuclei. Smears of 16 of the 17 patients with glaucoma stained by the Papanicolaou method illustrated striking tinctorial changes.

Staining of rabbit eosinophil and pseudoeosinophil leukocytes.

Air-dried rabbit blood was stained by HE, PAS and a modification of the Undritz II method. Eosin stained granules red in the eosinophil leukocytes. PAS was negative and the modified Undritz method failed to give consistent results.

Pseudoeosinophilic leucocyte response to denuding the rabbit cornea.

After scraping the epithelium from the rabbit's cornea, pseudoeosinophilic leucocytes appeared in the limbic area within one hour and began to advance, presumably by means of pseudopodia, into the anterior corneal stroma. The cytoplasmic granules of these cells were intensely stained by eosin and by the Undritz peroxidase method during the first hour, but were not stained by the PAS method until the eighteenth hour. Electron microscopy failed to show crystalloids in the granules.