A multiple fluorescein-based turn-on fluorophore (FHCS) identified for simultaneous determination and living imaging of toxic Al and Zn by improved Stokes shift.

A multiple turn-on fluorophore (FHCS), combining fluorescein, hydrazone, cyanuric chloride and salicylaldehyde chromone into a molecule, was identified and developed based on density functional theoretical calculation. It was expected that FHCS could express exclusive fluorescent signals and improved Stokes shifts when chelating Al or Zn. After it was synthesized and characterized in detail, it was noted that FHCS could turn-on fluorescently discriminate trace Al and Zn under the optimized conditions, i.