Publications by authors named "Shangke Wang"
Article Synopsis
- Triclocarban (TCC) is a widely used antibacterial agent that is harmful to the environment and can accumulate in the human body through various exposures, leading to concerns about its safety and reproductive toxicity.
- Research on the impact of TCC on early embryonic development in mice revealed that it affects development in a dose-dependent manner, with thousands of genes being differentially expressed, particularly impacting processes like redox reactions and DNA damage.
- TCC exposure increased DNA methylation and altered histone modifications, causing oxidative stress and ultimately disrupting zygotic genome activation and the degradation of maternal genes in 2-cell embryos.
Histone modifications are critical epigenetic indicators of chromatin state associated with gene expression. Although the reprogramming patterns of H3K4me3 and H3K27me3 have been elucidated in mouse and human preimplantation embryos, the relationship between these marks and zygotic genome activation (ZGA) remains poorly understood. By ultra-low-input native chromatin immunoprecipitation and sequencing, we profiled global H3K4me3 and H3K27me3 in porcine oocytes and in vitro fertilized (IVF) embryos.
View Article and Find Full Text PDFEarly embryos undergo extensive epigenetic reprogramming to achieve gamete-to-embryo transition, which involves the loading and removal of histone variant H2A.Z on chromatin. However, how does H2A.
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