Three-dimensionally ordered macroporous iron oxide for removal of H2S at medium temperatures.

A series of iron oxide sorbents with novel structures of three-dimensionally ordered macropores (3DOM), ranging in size from 60 to 550 nm, were fabricated and creatively used as sorbents for the removal of H2S at medium temperatures of 300-350 °C. Evaluation tests using thermogravimetric analysis (TGA) and a fixed-bed reactor showed that, in comparison to the iron oxide sorbent prepared by a conventional mixing method, the fabricated iron oxide sorbent with a 3DOM structure exhibited much higher reactivity and efficiency, as well as high sorbent utilization with low regeneration temperature. The excellent performance of 3DOM iron oxide as a sulfur sorbent is attributed to its special texture, i.

Rapid and ultrasensitive Salmonella Typhimurium quantification using positive dielectrophoresis driven on-line enrichment and fluorescent nanoparticleslabel.

This paper describes a convenient and sensitive methodology for Salmonella Typhimurium (S. Typhimurium) detection in microfluidic channels using positive dielectrophoresis (pDEP) driven on-line enrichment and fluorescent nanoparticles label. In the protocol, S.

Expression and characterization of a novel enantioselective lipase from Aspergillus fumigatus.

A 1,080-bp cDNA (CGMCC 2873) encoding of a cold-active lipase of Aspergillus fumigatus (AFL67) was cloned and expressed in Escherichia coli for the first time. The new lipase, AFL67, was one-step purified by 8.30 folds through Ni-NTA affinity chromatography with a recovery of 86.

Improved synthesis of 2'-deoxyadenosine and 5-methyluridine by Escherichia coli using an auto-induction system.

Nucleoside analogues are used widely for the treatment of viral diseases and cancer, however the preparation of some important intermediates of these nucleoside analogues, including 2'-deoxyadenosine (dAR) and 5-methyluridine (5-MU), remains inconvenient. To optimize the synthesis of dAR and 5-MU, recombinant strains and auto-induction medium were employed in this study. E.

Expression and characterization of a novel lipase from Aspergillus fumigatus with high specific activity.

A novel lipase gene from Aspergillus fumigatus, afl1-1, was cloned and expressed with a molecular mass of 38 kDa in Escherichia coli for the first time. The recombinant lipase had a preference for short carbon chain p-nitrophenyl esters, especially toward C2 p-nitrophenyl ester and exhibited potent hydrolysis activity that had not been observed. The optimum pH and temperature of this new enzyme were 8.

[Effects and mechanism of decorin on the proliferation of HuH7 hepatoma carcinoma cells in vitro].

Aim: To investigate the effects and mechanism of decorin( DCN) on the proliferation of HuH7 hepatoma carcinoma cell line in vitro.

Methods: Hepatoma carcinoma cells was cultured with DCN in different concentration (0, 25, 50, 75, 100, 125, 150, 200 microg/L) for different time(12, 24, 48, 72 h and 2 weeks). Cell activities were studied by MTT and clone test.

Association between epidermal growth factor polymorphism and esophageal squamous cell carcinoma susceptibility.

Genetic factors are known to be important in the development of esophageal squamous cell carcinoma (ESCC). Epidermal growth factor (EGF) can activate several signaling pathways leading to proliferation, differentiation, and tumorigenesis of epithelial tissues by binding with its receptor. Interindividual variations in EGF production were genetically contributed to EGF +61 G/A polymorphism.

Immunolocalization of matrix proteins in nacre lamellae and their in vivo effects on aragonitic tablet growth.

How matrix proteins precisely control the growth of nacre lamellae is an open question in biomineralization research. Using the antibodies against matrix proteins for immunolabeling and in vivo experiments, we investigate the structural and functional roles of EDTA-soluble matrix (SM) and EDTA-insoluble matrix (ISM) proteins in nacre biomineralization of the pearl oyster Pinctada fucata. Immunolabeling reveals that a SM protein, nacrein, distributes within aragonitic tablets and intertabular matrix.

Investigation on activated semi-coke desulfurization.

An activated semi-coke with industrial-scale size was prepared by high-pressure hydrothermal chemistry activation, HNO3 oxidation and calcination activation in proper order from Inner Mongolia Zhalainuoer semi-coke, which is rich in resource and cheap in sale. SO2 adsorption capacity on this activated semi-coke was assessed in the fixed bed in the temperature range of 60-170 degrees C, space velocity range of 500-1300 h(-1), SO2 concentration of 1000-3000 ppmv, and N2 as balance. The surface area, elemental and proximate analysis for both raw semi-coke and activated semi-cokes were measured.