Kinetic characterization of an intestinal trefoil factor receptor.

Objective: To determine whether intestinal epithelial cells have a receptor for intestinal trefoil factor and characterize receptor-ligand binding kinetics.

Methods: Radioligand binding assays were performed to characterize the binding kinetics between [(125)I]-labeled ITF and IEC-6, HT-29, Caco2 and HaCaT cells. The K d, Bmax and other kinetic variables describing the interaction between ITF and its potential receptors were determined.

Effects of glycine supplementation on myocardial damage and cardiac function after severe burn.

Background: Glycine has been shown to participate in protection from hypoxia/reoxygenation injury. However, the cardioprotective effect of glycine after burn remains unclear. This study aimed to explore the protective effect of glycine on myocardial damage in severely burned rats.

Effects of glutamine treatment on myocardial damage and cardiac function in rats after severe burn injury.

Treatment with glutamine has been shown to reduce myocardial damage associated with ischemia/reperfusion injury. However, the cardioprotective effect of glutamine specifically after burn injury remains unclear. The present study explores the ability of glutamine to protect against myocardial damage in rats that have been severely burned.

[Influence of human trefoil factor 1 (hTFF1) fusion protein on gastric mucosal lesion in mice after burn injury].

Objective: To observe the effect of hTFF1 fusion protein on gastric mucosal lesion in mice after burn injury.

Methods: The pET32alpha-hTFF1 vector were transfected into E.coli Origami B (DE3), and rhTFF1 fusion protein was expressed by IPTG induction.

[Protective effects of glycyl-glutamine dipeptide supplement on the heart function in burn rats].

Objective: To investigate the protective effects of glycyl-glutamine dipeptide supplement on the function of myocardial dynamics in severely burned rats, and to explore its mechanism.

Methods: One hundred and thirty-six Wistar rats were randomly divided into five groups: i. e, control group (C, n = 8, without burns), burn group (B, n = 32), Gln group (Gln, n = 32), Gly group (Gly, n = 32) and Gly-Gln group (Gly-Gln, n = 32).