Publications by authors named "Shang-Min Tu"
Article Synopsis
- 3-Methylitaconate-Delta-isomerase (Mii) is an enzyme in Eubacterium barkeri that catalyzes the conversion of (R)-3-methylitaconate to 2,3-dimethylmaleate and can also isomerize itaconate to citraconate, albeit less efficiently.
- The gene for Mii was successfully cloned and expressed in Escherichia coli, producing a protein with activity comparable to the natural enzyme, and its crystal structure revealed a tetrameric arrangement with distinct conformations.
- The enzyme features an active site with residues Lys62, Cys96, and others that play key roles in its function, showing structural
