[Construction and Identification of the cDNA Expression Library for Human Esophageal Cancer Cells].

Objectives: To construct a cDNA phage expression library for human esophageal cancer cells.

Methods: After the total RNA were obtained from esophageal cancer cells, the mRNA were separated with magnetic beads adsorption method, and the single-strand and double-strand cDNA were synthesized through reverse transcription. With the undesirable cDNA fragments removed, the remaining cDNA (linked withR1 aptamer and phosphorylated its 5'end) combined with the carrier of T7 Select10-3b.

[Combination of phage display and SEREX for screening early lung cancer associated antigens].

Objective: To screen out effective lung cancer associated antigens for early diagnosis in order to improve the level of early diagnosis.

Methods: A T7 phage display cDNA library of human early lung cancer was developed. And then differential phage clones were picked out to be sequenced and bioinformatically analyzed.