Development of Rapid Detection Methods for f. sp. in Melon Seeds.

Melon ( L.) is a global commercial crop that is sensitive to seed-borne wilt infections caused by f. sp.

Rapid Detection of Using Insulated Isothermal PCR and a Rapid, Simple DNA Preparation Protocol.

We developed an insulated isothermal PCR (iiPCR) method for the efficient and rapid detection of (Fo), which is a fungus that infects various hosts and causes severe crop losses. The Fo iiPCR method was sensitive enough to detect up to 100 copies of standard DNA template and 10 fg of Fo genomic DNA. In addition, it could directly detect 1 pg of mycelium and 10 spores of Fo without DNA extraction.

Using deep learning to identify maturity and 3D distance in pineapple fields.

Pineapples are an important agricultural economic crop in Taiwan. Considerable human resources are required to protect pineapples from excessive solar radiation, which could otherwise lead to overheating and subsequent deterioration. Note that simple covering all of the fruit with a paper bag is not a viable solution, due to the fact that it makes it impossible to determine whether the fruit is ripe.

First report of leaf brown blight caused by Neopestalotiopsis formicarum on jabuticaba in Taiwan.

Jabuticaba (Plinia cauliflora (Mart.) Kausel) was originated from Brazil (Lorenzi 2000). The production of jabuticaba is growing globally as its value in the food and pharmaceutical industries (Benvenutti 2021).