Acute restraint increases varicosity density and reduces the inter-varicosity distance in NADPH diaphorase-containing neurons in the rat dorsolateral periaqueductal gray matter.

The periaqueductal gray (PAG) is important for the organization of organismal response to different types of stress and painful stimuli. Its dorsolateral (dlPAG) column is distinctly characterized by the presence of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d), which in many brain regions, is an indication of constitutive nitric oxide (NO) synthase (NOS)-containing neurons. Different stress paradigms activate the dlPAG NOS machinery presumably by a presynaptic influence of NO on dlPAG neurons to modulate the nuclear dynamics to elicit an appropriate response.