Revolutionising Cancer Immunotherapy: Advancements and Prospects in Non-Viral CAR-NK Cell Engineering.

The recent advancements in cancer immunotherapy have spotlighted the potential of natural killer (NK) cells, particularly chimeric antigen receptor (CAR)-transduced NK cells. These cells, pivotal in innate immunity, offer a rapid and potent response against cancer cells and pathogens without the need for prior sensitization or recognition of peptide antigens. Although NK cell genetic modification is evolving, the viral transduction method continues to be inefficient and fraught with risks, often resulting in cytotoxic outcomes and the possibility of insertional mutagenesis.

The benefits of physical literacy for human flourishing: A machine learning-based exploration of adolescents.

Physical literacy is a multidimensional concept considered fundamental for lifelong participation in physical activity. Although theories on the relationship between physical literacy and human flourishing have been proposed, no comprehensive study of this relationship in adolescents has been found. This study aimed to predict human flourishing variables (physical activity, physical health, mental health, and academic performance) that correlate highly with adolescents' physical literacy.

Clinical value of esketamine combined with ropivacaine in rebound pain after brachial plexus block in patients with upper limb fractures.

Objective: To analyze the clinical value of the combination of esketamine and ropivacaine in alleviating rebound pain in patients with upper limb fractures following brachial plexus block.

Methods: A total of 149 patients with unilateral upper limb fractures who underwent open reduction and internal fixation surgery under brachial plexus block anesthesia from November 2021 to August 2022 were selected as the subjects for the study and randomly divided into the esketamine group (RNK group) and the ropivacaine group (R group). The incidence of rebound pain at 48 h postoperatively, intraoperative mean arterial pressure (MAP) and heart rate (HR), the onset time and duration of blockade, the Numeric Rating Scale (NRS) scores for pain at rest and with activity during the perioperative period, the dosage, numbers of compressions, and effective compressions of postoperative patient-controlled analgesia with sufentanil, and occurrence of adverse reactions were assessed and compared between the two groups.

Research on stroke patients' perception of recurrence risk: A scoping review protocol.

Introduction: Stroke, a major global cause of death and disability, has a high recurrence rate that significantly affects patients' physical, psychological, and economic well-being. Despite the importance of health risk perception in preventive measures, most stroke patients struggle to accurately assess the risk of recurrence. Current research on stroke recurrence risk perception is still exploratory, with a lack of systematic understanding of the influencing factors.

Comparative Chloroplast Genome Study of in China Sheds Light on Plastome Characterization and Phylogenetic Relationships.

Background: Mill., a morphologically diverse herbaceous perennial genus of Zingiberaceae, is distributed mainly in tropical to warm-temperate Asia. In China, species of have crucial medicinal, edible, and horticultural values; however, their phylogenetic relationships remain unclear.

Generation, Characterization, and Preclinical Studies of a Novel NKG2A-Targeted Antibody BRY805 for Cancer Immunotherapy.

Immuno-oncology has revolutionized cancer treatment, with NKG2A emerging as a novel target for immunotherapy. The blockade of NKG2A using the immune checkpoint inhibitor (ICI) monalizumab has been shown to enhance the responses of both NK cells and CD8+ T cells. However, monalizumab has demonstrated limited efficacy in in vitro cytotoxic assays and clinical trials.

A near infrared fluorescent probe for hypoxia based on dicyanoisophorone and its application in Hela cells imaging.

Hypoxia will accelerate tumors metastasis and deterioration, thereby limiting the effects of chemotherapy or radiotherapy. Thus, developing efficient techniques for detecting hypoxia in tumor cells is extremely important for cancer diagnosis and therapy. In this work, we reported a dicyanoisophorone-based probe (DCI-Azo) that specifically switched on its near infrared emission with hypoxia up-regulated azo-reductase (AzoR).

Mechano-assisted strategies to improve cancer chemotherapy.

Chemotherapy remains a cornerstone in cancer treatment; however, its effectiveness is frequently undermined by the development of drug resistance. Recent studies underscores the pivotal role of the tumor mechanical microenvironment (TMME) and the emerging field of mechanical nanomedicine in tackling chemo-resistance. This review offers an in-depth analysis of mechano-assisted strategies aimed at mitigating chemo-resistance through the modification of the TMME and the refinement of mechanical nanomedicine delivery systems.

Urine-based SERS and multivariate statistical analysis for identification of non-muscle-invasive bladder cancer and muscle-invasive bladder cancer.

Bladder cancer (BC) is an epidemiological urologic malignancy that continues to increase each year. Early diagnosis and prognosis monitoring is always significant in clinical practice, especially in distinguishing non-muscle-invasive bladder cancer (NMIBC) from muscle-invasive bladder cancer (MIBC), due to the various depths of tumor invasion related to different therapeutic schedules and recurrence rates. Common diagnostic approaches are too invasive or generally inefficient in accuracy and specificity.

MnO-triggered wavelength-changeable and rapid-response fluorescence sensor for paper-based on-site sensing of tyrosinase activity in potato.

Rapid-response in situ fluorogenic reactions in aqueous solution are important for designing sensitive and stable sensing platforms. Herein, a wavelength-changeable and rapid-response (within 5 s) fluorescence sensing platform for monitoring tyrosinase (TYR) activity is constructed. The developed assay is based on TYR catalyzing the hydroxylation of mono-phenol to o-diphenol and MnO-triggered fluorogenic between dopamine (DA) and phenol derivatives in aqueous solution.

Contrast-enhanced endoscopic ultrasound for differential diagnosis of autoimmune pancreatitis: a meta-analysis.

To assess the diagnostic value of contrast-enhanced endoscopic ultrasound (CE-EUS) for autoimmune pancreatitis and other solid pancreatic masses. A systematic search of PubMed, Embase, and Web of Science was performed from inception to October 2022. We calculated individual and pooled sensitivities and specificities to determine the diagnostic ability of CE-EUS.

Photocatalytic Tricyclization of Enediynes for the Synthesis of Chromenoquinolines in Continuous Flow.

A photocatalyzed radical-initiated tricyclization of enediynes was developed to synthesize a range of hitherto unknown sulfonated chromenoquinolines with moderate to excellent yields under continuous operational conditions. The reaction mechanism involves a complex series of steps including alkyl radical capture by sulfur dioxide (SO), radical addition, 6--/6--/6-- tricyclization, and single electron transfer (SET). This cascade of reactions results in the direct formation of a pentacyclic framework structure, along with the creation of up to three new rings and five chemical bonds in a single step.

SUBSTANDARD STARCH GRAIN7 regulates starch grain size and endosperm development in rice.

Starch is synthesized as insoluble, semicrystalline particles within plant chloroplast and amyloplast, which are referred to as starch grains (SGs). The size and morphology of SGs in the cereal endosperm are diverse and species-specific, representing a key determinant of the suitability of starch for industrial applications. However, the molecular mechanisms modulating SG size in cereal endosperm remain elusive.

Assessment of neutrophil to lymphocyte ratio, platelet to lymphocyte ratio and systemic immune-inflammatory index, as diagnostic markers for neonatal sepsis.

Objective: To assess the neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR) and systemic immune-inflammatory index (SII), as diagnostic markers for neonatal sepsis.

Methods: This retrospective study involve neonates with sepsis and healthy neonates as controls. NLR, PLR, and SII were compared between groups.

Optimal management options for esophageal gastrointestinal stromal tumors (E-GIST).

Objective: To explore the different treatment modalities for esophageal gastrointestinal stromal tumors (E-GIST) and their respective applicability and clinical outcomes.

Methods: This is a retrospective study in which consecutive patients diagnosed with E-GIST at our hospital from January 2017 to August 2023 were included. The clinical characteristics of all the patients as well as long-term quality of life were recorded and analyzed.

The supportive care needs of stroke patients: A scoping review.

The aims of the current review were to identify the current supportive care needs of stroke patients, categorize those needs according to the supportive care needs framework (SCNF), and to form a SCNF of stroke patients. Preferred Reporting Items for Systematic Reviews and Meta-Extension for Scoping Reviews (PRISMA-ScR) and Guidance for conducting systematic scoping reviews were followed. Ten databases were searched, including six English databases: PubMed, Embase, Web of Science, Cumulative Index to Nursing Allied Health Literature, Cochrane Library, and PsycINFO, and four Chinese databases: China National Knowledge Infrastructure, Wan Fang, China Biology Medicine Database and Chongqing VIP.

Molecular subtypes of ischemic heart disease based on circadian rhythm.

Coronary atherosclerotic heart disease (CAD) is among the most prevalent chronic diseases globally. Circadian rhythm disruption (CRD) is closely associated with the progression of various diseases. However, the precise role of CRD in the development of CAD remains to be elucidated.