[Preparation of a composite membrane with collagen scaffold material for tissue culture].

Objective: To prepare a composite membrane with collagen scaffold material for tissue culture.

Methods: Pure collagen membrane, collagen/hyaluronic acid (HA) membrane, collagen/chitosan membrane and collagen/chitosan/ HA membrane (abbreviated as composite collagen membrane) were prepared respectively, and their respective biodegradation time and the number of the cells proliferated on them were examined in vitro.

Result: The biodegradation time of pure collagen, collagen/HA and composite collagen membranes in vitro was 1 380, 1 376 and 1 560 min respectively, and the number of cells proliferated on the collagen/HA membrane and the composite collagen membrane were greater than that on the pure collagen membrane.

Preparation of polylactic acid/chitin composite material and its safety evaluation by animal experiments.

Objective: To prepare a scaffold material with good biocompatibility and biodegradability by compounding polylactic acid (PLA) and chitin.

Methods: After preparation of PLA from lactic acid, the compounding of PLA and chitin was carried out by dissolving these 2 materials in one solution for reaction. The composite material was obtained and molded after the solvent was evaporized, and the safety tests of this resultant material were conducted in guinea pigs and New Zealand rabbits, respectively.

Preparation and biocompatibility of tissue-engineered scaffold materials based on collagen.

Objective: To prepare a tissue-engineered scaffold material using collagen as the matrices and to study the blood compatibility and tissue biocompatibility of this material.

Methods: Physical, chemical and physical/chemical methods were used for the crosslinking of the collagen.

Results: Dynamic blood clotting tests indicated that the blood clotting index (BCI) of the crosslinked collagen materials prepared by different means decreased as their contact with the blood was prolonged, and the collagen material obtained after crosslink through 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide method showed the highest BCI after contact with the blood within certain length of time.