[Study on mimotopes of E.coli lipopolysaccharide 2630].

Aim: To screen mimotopes of E.coli lipopolysaccharide(LPS) 2630 from c7c phage display peptide library.

Methods: The LPS mimotopes were screened from c7c phage display peptide library by using affinity chromatograph-purified polyclonal antibody against E.

[Screening and identification of human telomerase reverse transcriptase epitopes from random phage display peptide library].

Objective: To identify and characterize the epitopes of human telomerase reverse transcriptase (hTERT).

Methods: A random phage displayed dodecapeptide library was screened with anti-hTERT antibody. The selected phage clones were identified by sandwich enzyme-linked immunosorbent assay, anti-hTERT antibody blocking assay and competitive inhibition assay.

[Screening of mimotope of Salmonella typhimurium lipopolysaccharide from phage-displayed peptide].

Objective: To identify and characterize the mimotope of lipopolysaccharide (LPS) from cyclic 7-mer phage peptide library.

Methods: Cyclic 7-mer phage-displayed peptide library was screened using monoclonal antibody 2F4 (mAb 2F4) against Salmonella typhimurium LPS as the target, and the selected clones were tested by sandwich enzyme-linked immunosorbent assay (ELISA) and specific antigen inhibition ELISA.

Results: After 3 round of screening, 34 of the 38 selected clones were identified as positive for binding to mAb 2F4.

[Screening of short peptides that bind specifically to osteosarcoma cells by phage-displayed peptide library].

Objective: To obtain short peptides which bind specifically to osteosarcoma cells os-732 by means of screening from 12 peptide libraries.

Methods: Osteosarcoma cells os-732 were used as the target cells and osteoblasts as the absorber cells for subtraction biopanning from a 12-mer peptide phage-display library. After 3 rounds of screening, the positive phage clones were identified by cell enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, and the amino acid sequences were deduced by DNA sequencing.