Molecular and functional characterization of sirt4 and sirt6 in Megalobrama amblycephala under high glucose metabolism.

We analyzed the sequences of sirt4 and sirt6 and their changes in expression after oral glucose administration in blunt snout bream (Megalobrama amblycephala). We cloned sirt4 and sirt6 and found that their full-length cDNA sequences were 1530 bp and 1723 bp, respectively; their amino acid sequences were 93% and 92% identical to Danio rerio. Megalobrama amblycephala were fed a high glucose solution (3 g/kg).