Niclosamide attenuates inflammatory cytokines via the autophagy pathway leading to improved outcomes in renal ischemia/reperfusion injury.

Renal ischemia/reperfusion (I/R) injury is a debilitating condition that leads to loss renal function and damage to kidney tissue in the majority of patients with acute kidney disease. Previous studies have indicated that autophagy serves a protective function in renal I/R injury. In the present study, the effect of the anthelmintic niclosamide in the regulation of inflammatory responses in kidney I/R was investigated.

[Preparation Technique of Tetraploid of Dioscorea zingiberensis Synthetic Seed Based on Embryogenic Callus].

Objective: To provide technical support for industrialization promotion of tetraploid of Dioscorea zingiberensis, the manufacturing method for synthetic seeds of tetraploid of Dioscorea zingiberensis was established and the correlated influential factors were studied.

Methods: By taking embryogenic calluses of tetraploid of Dioscorea zingiberensis as propagation materials, the influential factors such as components of artificial endosperm, seed coats,storage conditions and germination materials on germination and seedling of the synthetic seeds were evaluated.

Results: When 4% alginate +2% CaCl2 + 2% chitosan was served as seed coat materials, and 1/2 MS +0.

[Genetic stability study on autotetraploid plant of Dioscorea zingiberensis].

Objective: To study the genetic stability of autotetraploid plant of Dioscorea zingiberensis.

Methods: The chromosome of root-tip was determined by photomicroscope, and the agronomic characters were observed in the period of stable growth. The protein content was determined and the experiment of protein polyacrylamide gel electrophoresis was carried out.

Germplasm preservation in vitro of Polygonum multiflorum Thunb.

Background: The root of Polygonum multiflorum Thunb. is a common traditional Chinese medicine. In recent years, the wild resources of P.

[Studies on adventitious root induction in vitro and suspension culture of Polygonum multiflorum].

To achieve sustainable resources use of Polygonum multiflorum, adventitious roots were efficiently induced and cultured by suspension culture. In order to obtain optimal medium for induction adventitious roots from the young stems of P. multiflorum, MS medium was optimized by supplementing with different concentrations of sucrose and plant growth substances.

[Study on inducing embryogenic callus of Dioscorea zingiberensis by orthogonal design].

Objective: To select the suitable medium to induce embryogenic callus of Dioscorea zingiberensis.

Methods: Plantlet of Dioscorea zingiberesis in vitro was obtained by using apical meristem as explant. The different parts of the plantlets were cultured to select the best explant used for inducing callus and embryoids.

[Optinization of rapid propagation technique and induction and identification of autotetraploid of Polygonum multiflorum].

Objective: To establish and optimize the rapid propagation system of Polygonum multiflorum, as well as explore method for induction and identification of autotetraploid.

Method: Propagation medium was optimized by orthogonal test. The buds were immersed in colchicine solution with different concentrations for different time to select induction conditions for autotetraploid of P.

[Effects of 1, 25(OH)(2);D(3); on parathyroid hormone induced transdifferentiation and TGF-β(1); expression in cultured human renal tubular epithelial cells].

Aim: To explore the effects of 1, 25(OH)(2);D(3); on parathyroid hormone (PTH) induced transdifferentiation and TGF-β(1); expression in cultured human renal tubular epithelial cells.

Methods: HK-2 cells were cultured in DMEM/F12 medium supplemented with 50 mL/L FBS. Cells were divided into three groups.

Generation of autotetraploid plant of ginger (Zingiber officinale Rosc.) and its quality evaluation.

Background: Zingiber officinale Rosc. is not only an important medical plant in China, but also one of the most commonly used plant spices around the world. Early researches in Z.

Tissue culture and generation of autotetraploid plants of Sophora flavescens Aiton.

Background: Sophora flavescens Aiton is an important medicinal plant in China. Early in vitro researches of S. flavescens were focused on callus induction and cell suspension culture, only a few were concerned with in vitro multiplication.

Up-regulation of licochalcone A biosynthesis and secretion by Tween 80 in hairy root cultures of Glycyrrhiza uralensis Fisch.

We evaluated the effect of Tween 80 as elicitor on licochalcone A from hairy root cultures of Glycyrrhiza uralensis Fisch. After a 15-days treatment with 2% Tween 80, hairy roots still grew well and produced higher levels of licochalcone A and total flavonoids than the control (without treatment). Licochalcone A content and total flavonoid content were 3.

In vitro tetraploid induction and generation of tetraploids from mixoploids in Dioscorea zingiberensis.

This article describes an efficient colchicine-mediated technique for the in vitro induction of tetraploids in Dioscorea zingiberensis and its confirmation by flow cytometry. Buds immersed in 0.2% colchicine solution for 36 hours prior to culture induced as high as 35.

[Analysis of biochemical indices and efficient ingredients in autotetraploid of Dioscorea zingibiernsis].

Objective: To improve the yield and quality of Dioscrea zingibiernsis.

Methods: The Biochemical indices and the diosgenin's content were analysed in the autotetraploid lines.

Results: The results showed that the activities of APX, SOD and POD in most of autotetraploid lines were higher than that in diploid line or close to it, there was also difference between autotetraploid lines and control lines in the SDS-PAGE of soluble proteins.

Enhanced flavonoid production in hairy root cultures of Glycyrrhiza uralensis Fisch by combining the over-expression of chalcone isomerase gene with the elicitation treatment.

Economically important compounds, such as licorice flavonoids, are present in insufficient amounts in the hairy roots. To overcome this problem, we took the transgenic approach combined with the elicitation technique to increase the flavonoid production. The Glycyrrhiza uralensis Fisch cDNA encoding chalcone isomerase gene (chi) was over-expressed in hairy roots of G.

[Application and study of HPLC fingerprint of Lonicera japonica for identification of geo-authenticity].

Objective: To establish a chromatographic fingerprint of Lonicera japonica and apply it to identify geo-authentic of L. japonica.

Method: The HPLC was applied in chromatographic separation and data were analysed by "computed aided similarity evaluation" software.