Fabrication of 3-D gel microarrays directly with raw polymerase chain reaction products by heat-directed polymerization.

In this study, a new method for fabricating 3-D polyacrylamide gel microarrays was presented. In the method, a 3-D gel microarray was successfully fabricated by directly heating the slides with arrayed prepolymer at 80 degrees C for 2 min. The prepolymer on the microarray only contained 40% v/v raw/unpurified PCR products, 1.

[Cloning of gene fragment of estrogen receptor-beta and its expression in mouse embryo].

In order to study the expression and regulation effects of estrogen receptor-beta (ERbeta) in the development of mouse embryo, the primer of ERbeta was designed, the ERbeta fragment was first obtained by RT-PCR and subcloned into plasmids pGEM- 3Z, then the recombinant plasmids were linearized with the restriction enzymes of EcoRand Hind. Using Sp6 and T7 RNA polymerase, the digoxigenin(dig) labeled sense and anti-sense probes were transcriped in vitro, respectively. Then the expression of ERbeta in mouse embryo was examined with the probes by whole-mount in situ hybridization.

Purple sweet potato color repairs d-galactose-induced spatial learning and memory impairment by regulating the expression of synaptic proteins.

Purple sweet potato color (PSPC), a class of naturally occurring anthocyanins used to color food (E163), has been reported to possess a variety of biological activities, including anti-oxidant, anti-tumor, and anti-inflammatory. The effect of PSPC on the spatial learning and memory of mice treated with d-galactose (d-gal) was evaluated by the Morris water maze; d-gal-treated mice had decreased performance compared with mice in the vehicle and PSPC groups, while the PSPC+d-gal group showed significantly shortened escape latency to platform, increased swimming speed, more target quadrant search time and more platform crossings as compared with the d-gal group. Brain functions, such as memory formation and recovery of function after injury, depend on proper regulation of the expression levels of the pre- and post-synaptic proteins.

Short-term normalization of ventricular repolarization by transcatheter ablation in a patient with suspected Brugada Syndrome.

We report a 30 year old male without structural heart disease who presented with recurrent nocturnal syncope and aborted sudden cardiac death. 12-lead ECG showed elevated ST in inferior leads and short coupled premature ventricular complexes (PVCs). Propafenone challenge suggested a diagnosis of an atypical Brugada syndrome.

[Tpeak-Tend interval and risk of cardiac events in patients with Brugada syndrome].

Objective: To evaluate the possible association between Tpeak-Tend (Tp-e) interval in surface standard ECG and cardiac events in patients with Brugada syndrome (BrS).

Method: Tp-e interval in surface standard ECG was compared between BrS patients (n = 23, all males) and paroxysmal supraventricular tachycardia (PSVT) patients (n = 20, all males) as well as between BrS patients with (n = 16) or without (n = 7) cardiac events.

Results: There was significant difference in Tp-e interval between BrS patients and PSVT patients [(109.

Effects of resveratrol on number and activity of endothelial progenitor cells from human peripheral blood.

1. It has been well established that oestrogens can increase the number of endothelial progenitor cells (EPC) by anti-apoptotic effects. Resveratrol, a polyphenolic phytoalexin extracted from grapes and wine, has been reported to act as an oestrogen receptor agonist.

[Observation on the therapeutic effect of ear point combined therapy on common migraine at the remission stage and effects on plasma CGRP and ET in the patient].

Objective: To probe into the therapeutic effect of ear point combined therapy on common migraine at the remission stage and the mechanism.

Methods: Thirty-two cases of common migraine at the remission stage were treated with ear point combined therapy, including blood-letting at ear back, injection of autoblood into Fengchi (GB 20), Yanglingquan (GB 34), and pricking at ear points Nie, Yi-dan (pancreas and gallbladder), Shenmen, etc..

Epithelial K⁺ channels: driving force generation and K⁺ recycling for epithelial transport with physiological and clinical implications.

K(+) channels form a large family of membrane proteins that are expressed in a polarized fashion in any epithelial cell. Based on the transmembrane gradient for K(+) that is maintained by the Na(+)-K(+)-ATPase, these channels serve two principal functions for transepithelial transport: generation of membrane voltage and recycling of K(+). In this brief review, we will outline the importance of this ancient principle by examples of epithelial transport in the renal proximal tubule and gastric parietal cells.

Ursolic acid ameliorates cognition deficits and attenuates oxidative damage in the brain of senescent mice induced by D-galactose.

Ursolic acid (UA), a pentracyclic triterpene, is reported to have an antioxidant activity. Here we assessed the protective effect of UA against the d-galactose (D-gal)-induced neurotoxicity. We found that UA markedly reversed the D-gal induced learning and memory impairment by behavioral tests.

[Observation on therapeutic effect of electroacupuncture combined with functional training for treatment of peripheral nerve incomplete injury of upper limbs].

Objective: To search for the best program for treatment of peripheral nerve incomplete injury.

Methods: Ninety cases were randomly divided into a treatment group, a control group I and a control group II, 30 cases in each group. The treatment group were treated with electroacupuncture at Jianyu (LI 15), Hegu (LI 4), Quchi (LI 11), etc.

Reversible left ventricular dyssynchrony and dysfunction resulting from right ventricular pre-excitation.

We report observations in a 7-year-old girl with right ventricular pre-excitation due to an accessory atrioventricular pathway (AP), and depressed cardiac function. Echocardiographic findings consisted of left ventricular (LV) dilatation with asynchronous ventricular wall motion and diminished LV ejection fraction. Electrophysiological study revealed a para-Hisian AP.

Neuropharmacological profile of novel and selective 5-HT6 receptor agonists: WAY-181187 and WAY-208466.

One of the most recently identified serotonin (5-hydroxytryptamine (5-HT)) receptor subtypes is the 5-HT6 receptor. Although in-depth localization studies reveal an exclusive distribution of 5-HT6 mRNA in the central nervous system, the precise biological role of this receptor still remains unknown. In the present series of experiments, we report the pharmacological and neurochemical characterization of two novel and selective 5-HT6 receptor agonists.

[The alterations of nitric oxide synthase activity of ventricular cardiac muscle of rats in two septic shock models].

Aim: To observe the differences of hemodynamics and nitric oxide synthase(NOS) activity of ventricular cardiac muscle in two septic shock models and explore the possible mechanism.

Methods: Two rat models of septic shock[lipopolysaccharide(LPS)-induced and cecal ligation and puncture (CLP)-induced septic shock] were used. The hemodynamic parameters and nitric oxide synthase activity of ventricular cardiac muscle were measured.

[Electrophysiological findings and ablation strategies in patients with atrial tachyarrhythmias after left atrial circumferential ablation in the treatment of atrial fibrillation].

Objective: To report the electrophysiological findings and the ablation strategies in patients with atrial tachyarrhythmias (ATAs) or atrial fibrillation (AF) recurrence after left atrial circumferential ablation (LACA) in the treatment of AF.

Methods: 91 patients with AF had LACA procedure from April 2004 to May 2006, 19 of which accepted the second ablation procedure due to ATAs or AF recurrence. In all the 19 patients [17 male, 2 female, age 25 - 65 (53 +/- 12) years], 11 presented with paroxysmal AF before the first ablation procedure, 2 with persistent AF and 6 with permanent AF.

Transgenic RNAi depletion of claudin-16 and the renal handling of magnesium.

Tight junctions play a key role in mediating paracellular ion reabsorption in the kidney. Familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC) is a human disorder caused by mutations in the tight junction protein claudin-16. However, the molecular mechanisms underlining the renal handling of magnesium and its dysfunction causing FHHNC are unknown.

Direct inhibition of Ih by analgesic loperamide in rat DRG neurons.

Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are responsible for the functional hyperpolarization-activated current (I(h)) in dorsal root ganglion (DRG) neurons, playing an important role in pain processing. We found that the known analgesic loperamide inhibited I(h) channels in rat DRG neurons. Loperamide blocked I(h) in a concentration-dependent manner, with an IC(50) = 4.

Termination of polymorphic ventricular tachycardia storm by catheter ablation in a patient with cardiomyopathy induced by incessant idiopathic left ventricular tachycardia.

We detail findings in a patient with incessant idiopathic left ventricular tachycardia (ILVT), induced cardiomyopathy, and an "electrical storm" consisting of recurrent polymorphic ventricular tachycardia (PVT). Catheter ablation not only eradicated the ILVT, but also additionally suppressed recurrent PVT. These findings suggest that the recurrent PVT storm in this patient related to long-standing tachycardia-induced cardiac electrical remodeling that led to QT prolongation.

The relationship of ventricular dynamics and mitochondrial nitric oxide synthase activity in septic shock models.

The aim of the present study was to investigate the role of mitochondrial nitric oxide synthase (mtNOS) in the septic shock and analyze its relationship to ventricular contractility. Two models of septic shock [lipopolysaccharide (LPS)-induced and cecal ligation and puncture (CLP)-induced] were used. There was a significant depression of ventricular contractile parameters recorded in the late stage of the septic shock.

An overview of the integrated laboratory course of physiological sciences at Zhejiang University in China.

An integrated laboratory course of physiological sciences has been put into use at Zhejiang University School of Medicine since 1993. This course guides students in taking a scientific approach to understanding the contemporary issues in physiology, pathophysioiogy and pharmacology. Students learn to use a variety of basic experimental methods to laboratory work.

Dorsal column is not involved in the mechanism of the hypotensive effect by simulating acupuncture on rat hindlimb.

The present study investigated the role of the dorsal column (DC) in the mechanism of the hypotensive effect induced by simulating acupuncture on rat hindlimb. The femoral arterial pressure and electrocardiogram (ECG) of rats were recorded when the hypothalamic paraventricular nucleus (PVN) was electrically stimulated with or without DC lesion. Stimulation of the deep peroneal nerve (DPN) decreased the pressor response elicited by electrical stimulation of the PVN.

Endothelium-independent vasorelaxant effect of puerarin on rat thoracic aorta.

To investigate the vasorelaxant effect of puerarin, tension was recorded from rat thoracic aortic rings. Puerarin completely relaxed the contractions induced by phenylephrine (PE) in a concentration-dependent manner in endothelium-intact and endothelium-denuded rat aorta, while had no effect on those preconstricted by a high concentration of potassium chloride (60 mM). Also, puerarin had no effects on the transient contraction elicited by PE or caffeine in Ca2+- free medium.

Endothelium-independent vasorelaxant effect of lidocaine in rat aortic rings.

In the present study, lidocaine relaxed, in a concentration-dependent manner, the contractions induced by either phenylephrine or a high concentration of KCl (60 mM) in endothelium-intact rat aortic rings. Mechanical removal of endothelium did not significantly modify lidocaine-induced vasorelaxation. In endothelium-denuded aortic rings depolarized by 60 mM KCl, lidocaine inhibited Ca2+-induced contraction.

Hypoxia reoxygenation induces premature senescence in neonatal SD rat cardiomyocytes.

Aim: To investigate whether hypoxia reoxygenation induces premature senescence in neonatal Sprague-Dawley (SD) rat cardiomyocytes.

Methods: Cardiomyocytes were isolated from neonatal SD rat heart and identified by immunohistochemistry. The control cultures were incubated at 37 degree centigrade in a humidified atmosphere of 5% CO(2) and 95% air.