Caveolin-1 protects against liver injury and lipid accumulation in alcoholic fatty liver via ferroptosis resistance.

Alcoholic fatty liver (AFL) is one of the most common chronic liver diseases globally with complex and controversial pathogenesis. Recent evidence suggests that iron overload and lipid peroxidation are risk factors for AFL. Caveolin-1 (CAV1) is an important signal platform that can maintain lipid homeostasis during the development of non-alcoholic fatty liver.

Phosphorylation-driven regulation of SIRT1 in muscle senescence: Insights from molecular dynamics simulation and experimental validation.

Sirtuin 1 (SIRT1) is a key regulator of mitochondrial function and inflammatory responses, both of which are critical in the progression of muscle aging and sarcopenia. While SIRT1's activity is known to be regulated by post-translational modifications, the specific role of Ser46 phosphorylation has not been fully elucidated. In this study, we explored the effects of Ser46 phosphorylation on SIRT1's structural stability, subcellular localization, and downstream signaling in the context of muscle cell senescence.

Spatial pattern of attractiveness and road network accessibility of scenic spots in Guangdong Province based on network information.

With the progress of the Internet, the limitations of traditional scenic spots research in data collection, market evaluation and tourist experience analysis can be broken through, and a new way to evaluate the attractiveness of scenic spots has been opened up. Scenic spots, as a key factor for tourists to choose a destination, has a profound impact on tourism behavior. Focusing on Guangdong Province, this study used GIS spatial analysis and geographic detector to analyze the spatial distribution, influencing factors and forming mechanism of scenic spots in Guangdong Province.

NSUN2/ALYREF axis-driven mC methylation enhances PD-L1 expression and facilitates immune evasion in non-small-cell lung cancer.

Non-small-cell lung cancer (NSCLC) represents a highly prevalent form of malignancy. 5-methylcytosine (mC) methylation functions as a key post-transcriptional regulatory mechanism linked to cancer progression. The persistent expression of PD-L1 in tumor cells plays a pivotal role in facilitating immune evasion and promoting T-cell exhaustion.

Enhancing intact allergen protein detection in dairy products: Preventing fragmentation and aggregation with Sinapinic acid salt in MALDI-TOF MS analysis.

The development of efficient detection technology for dairy allergens represents a crucial concern for public health. In this study, a strategy utilizing sinapinic acid salt (SAS) for analyzing allergen proteins through MALDI-TOF MS is presented. SAS has proven to be a superior matrix compared to solid matrices, as it inhibits aggregation and prevents fragmentation, thereby yielding more reliable molecular mass values of intact allergen proteins.

ROR1 CAR-T cells and ferroptosis inducers orchestrate tumor ferroptosis via PC-PUFA2.

Background: Lung cancer, particularly non-small cell lung cancer (NSCLC), has high recurrence rates and remains a leading cause of cancer-related death, despite recent advances in its treatment. Emerging therapies, such as chimeric antigen receptor (CAR)-T cell therapy, have shown promise but face significant challenges in targeting solid tumors. This study investigated the potential of combining receptor tyrosine kinase-like orphan receptor 1 (ROR1)-targeting CAR-T cells with ferroptosis inducers to promote ferroptosis of tumor cells and enhance anti-tumor efficacy.

SIV Env RhmAbs + N-803 at ART initiation prolongs viral decay without disrupting reservoir establishment in SIV-infected infant macaques.

The latent viral reservoir remains the major barrier to HIV cure, placing the burden of strict adherence to antiretroviral therapy (ART) on people living with HIV to prevent recrudescence of viremia. For infants with perinatally acquired HIV, adherence is anticipated to be a lifelong need. In this study, we tested the hypothesis that administration of ART and viral Envelope-specific rhesus-derived IgG1 monoclonal antibodies (RhmAbs) with or without the IL-15 superagonist N-803 early in infection would limit viral reservoir establishment in SIV-infected infant rhesus macaques.

Research Progress of Liquid Biopsy Based on DNA Methylation in Tumor Diagnosis and Treatment.

Liquid biopsy has been gradually applied to the clinical diagnosis and treatment of tumors because of its non-invasive and real-time reflection of the tumor status, as well as the convenience of sample collection, which allows the detection of primary or metastatic malignant tumors and reflects the heterogeneity of the tumors. DNA methylation, which is a type of epigenetic modification, is essential in the progression of tumors. This review introduces the common DNA methylation analysis methods and discusses their advantages and disadvantages, focusing on the new progress of DNA methylation-based liquid biopsy in tumor diagnosis and treatment.

The crucial quality marker of Panax ginseng: Glycosylated modified ribonuclease-like storage protein.

Panax ginseng C.A.Mey is a famous natural herbal medicine worldwide.

Perceptions of Cognitive Load and Workload in Nurse Handoffs: A Comparative Study Across Differing Patient-Nurse Ratios and Acuity Levels.

Medical errors, often resulting from miscommunication and cognitive lapses during handoffs, account for numerous preventable deaths and patient harm annually. This research examined nurses' perceived workload and cognitive load during handoffs on hospital units with varying patient acuity levels and patient-nurse ratios. Conducted at a southeastern US medical facility, the study analyzed 20 handoff dyads using the National Aeronautics and Space Administration Task Load Index to measure perceived workload and cognitive load.

Supplementation with NS8 alleviated behavioral, neural, endocrine, and microbiota abnormalities in an endogenous rat model of depression.

Introduction: Major depressive disorder is a condition involving microbiota-gut-brain axis dysfunction. Increasing research aims to improve depression through gut microbiota regulation, including interventions such as probiotics, prebiotics, and fecal microbiota transplants. However, most research focuses on exogenous depression induced by chronic stress or drugs, with less attention given to endogenous depression.

Mapping fine-scale carbon sequestration benefits and landscape spatial drivers of urban parks using high-resolution UAV data.

Urban parks offer dual ecological benefits by increasing carbon sinks and reducing carbon emissions and are considered an important natural strategy for cities to achieve "double carbon" goals. However, rapid, efficient, and accurate quantification of the carbon sequestration benefits of urban parks poses a significant challenge. To address this, we utilized unmanned aerial vehicle (UAV) multispectral data to construct large-area, high-resolution models of urban park carbon sequestration benefits, replacing traditional, time-consuming, and laborious large-scale field surveys.

Natural allelic variations confer drought tolerance and influence nodule formation in soybean.

Unlabelled: Soybean [ (L.) Merr.] is one of the most important, but a drought-sensitive, crops.

Role of the CARD8 inflammasome in HIV pathogenesis.

Human immunodeficiency virus (HIV) continues to be a significant global health challenge despite decades of research and advances in treatment. Substantial gaps in our understanding of the mechanisms of HIV pathogenesis and the host immune responses still exist. The interaction between HIV and these immune responses is pivotal in the disease progression to acquired immunodeficiency syndrome (AIDS).

Prunus persica (L.) Batsch blossom soluble dietary fiber synergia polyphenol improving loperamide-induced constipation in mice via regulating stem cell factor/C-kit, NF-κB signaling pathway and gut microbiota.

This study aimed to investigate the ameliorating effects of peach blossom soluble dietary fiber (PBSDF) and polyphenol (PBP) combinations on loperamide (Lop)-induced constipation in mice, together with the possible mechanism of action. The results demonstrated that the combined use of PBSDF and PBP could synergistically accelerate the gastrointestinal transit rate and gastric emptying rate, shorten first red fecal defecation time, accelerate the frequency of defecation, regulate the abnormal secretion of gastrointestinal neurotransmitters and pro-inflammatory cytokines, and down-regulate the expressions of AQP3 and AQP8. Western blotting and RT-qPCR analysis confirmed that PBSDF + PBP up-regulated the protein and mRNA expressions of SCF and C-kit in SCF/C-kit signaling pathway, and down-regulated pro-inflammatory mediator expressions in NF-κB signaling pathway.

Gut microbiota and healthy longevity.

Recent progress on the underlying biological mechanisms of healthy longevity has propelled the field from elucidating genetic modification of healthy longevity hallmarks to defining mechanisms of gut microbiota influencing it. Importantly, the role of gut microbiota in the healthy longevity of the host may provide unprecedented opportunities to decipher the plasticity of lifespan on a natural evolutionary scale and shed light on using microbiota-targeted strategies to promote healthy aging and combat age-related diseases. This review investigates how gut microbiota affects healthy longevity, focusing on the mechanisms through which gut microbiota modulates it.

Kasai Portoenterostomy, Successful Liver Transplantation, and Immunosuppressive Therapy for Biliary Atresia in a Female Baby: A Case Report.

Background: Biliary atresia (BA) is a severe neonatal progressive cholangiopathy of unknown etiology. A timely Kasai portoenterostomy (KPE) improves survival of the native liver in patients with BA, although liver transplantation remains the ultimate treatment for most (60%-80%) patients. However, postoperative adverse effects of liver transplantation may be significant.

PANoptosis: Novel insight into regulated cell death and its potential role in cardiovascular diseases (Review).

PANoptosis, a complex form of proinflammatory programmed cell death, including apoptosis, pyroptosis and necroptosis, has been an emerging concept in recent years that has been widely reported in cancer, infectious diseases and neurological disorders. Cardiovascular diseases (CVDs) are an important global health problem, posing a serious threat to individuals' lives. An increasing body of research shows that inflammation has a pivotal role in CVDs, which provides an important theoretical basis for PANoptosis to promote the progression of CVDs.

Crystal structural effects on up/down-conversion luminescence properties of GdInO:Tm,Yb perovskite phosphors for effective dual-mode anti-counterfeit applications.

Developing advanced luminescent materials that are recognizable under specified conditions provides better opportunity for reliable optical anti-counterfeiting techniques. In this work, to the best of our knowledge, novel GdInO:Tm,Yb perovskite phosphors with ultrafine sizes and rounded morphologies were successfully synthesized by a facile chemical precipitation route. Two-type perovskites with orthorhombic and hexagonal structures could be obtained by calcining the precursor at 850 and 1100 °C, respectively.

Discovery of potential anti- natural products and their mechanistic studies using machine learning and molecular dynamic simulations.

The structure-activity analysis (SAR) and machine learning were used to investigate potential anti- agents in a faster method. In this study, 24 oxygenated benzene ring components with inhibition capacity were confirmed by literature exploring and in-house experiments, and the SAR analysis suggested that the hydroxyl group position may affect the anti- activity. The 2D-MLR-QSAR model with 9 descriptors was further evaluated as the best model among the 21 models.