Assessment of Adipokines, CXCL16 Chemokine Levels in Patients With Rheumatoid Arthritis Combined With Metabolic Syndrome.

Objective: Rheumatoid arthritis (RA), which is a chronic systemic inflammatory disease, is associated with accelerated atherosclerosis and an increased risk of cardiovascular disease (CVD), but the causal factors have yet to be completely elucidated. The studies show that the prevalence of metabolic syndrome (MtS) was significantly higher in RA patients compared to the population. In RA and MetS inflammation and atherosclerosis are closely linked.

Biochemical markers of hypertension, prehypertension.

Objective: There are insufficient researches aimed at evaluating biochemical markers of mechanisms of formation of AH and lesion of target organs in hypertension and prehypertension. The aim of that research was to study the level of endothelial dysfunction markers and damage to the cardiovascular system in hypertension and prehypertension.

Patients And Methods: A cross-sectional study was performed among 938 people aged 18 to 65 years.

[EPIDEMIOLOGY, STRUCTURE AND ALGORITHM OF MANAGEMENT OF PREGNANT WOMEN WITH EXTRAGENITAL PATHOLOGY OF THERAPEUTIC PROFILE].

Purpose of the study - the study of the extragenital pathology (EGP) structure in pregnant women according to the requests for medical help. The screening survey was attended by 742 pregnant women. Average age was 28,4±5,5 years.

[Milk ultrafiltrate as a promising source of angiogenin].

The use of membrane technologies in the production of soft cheese (children's food) is associated with the appearance of up to 80% of angiogenin in the ultrafiltrate. An electrophoretically homogeneous preparation of angiogenin (MW approximately 17 kDa) was obtained from milk ultrafiltrate by two-stage ion-exchange chromatography. The yield of the angiogenin was approximately 60%, which corresponds to a 586-fold purification of the raw material.

[Angiogenin content in human milk in the early period of lactation].

The content of angiogenin in human milk and dairy produce for feeding of children of the first year of life is investigated. It is revealed, that the highest concentration of angiogenin in human milk takes place in colostric period (18.00 +/- 0.

[Immunoenzyme analysis of angiogenin in cow's milk].

Competitive enzyme immunoassay based on polyclonal antibodies can be used for determining the content of angiogenin in milk. These polyclonal antibodies had no cross-reactions with ribonuclease or other milk whey proteins. Milk angiogenin levels in samples taken fvom animals of a separate population varied from 2.

[Determination of angiogenin in cow's milk based on a competitive test "pancreatic RNAase/placental inhibitor of RNAase--angiogenin"].

Contents of active factor of blood vessel growth angiogenin was analyzed in cow's milk by the method of competitive test in system "pancreatic RNAase/placental inhibitor of RNAase-angiogenin". High level of RNAase in milk limits using this test. To avoid this limitation the method of RNAase elimination from milk was elaborated.

[Purification of angiogenin from cow's milk].

A new method for the purification of bovine angiogenin is proposed which is based on the use of CM-cellulose, CM-Toyopearl, and Butyl-Toyopearl. Electrophoretically homogeneous protein was obtained with a 49% yield and 12,000-fold purification.

[Quantitative determination of cattle angiogenin].

Polyclonal antibodies to a milk antigen were obtained by a standard immunization procedure. The possibility was demonstrated to use the peroxidase-antibody conjugate in a competitive immunoenzymatic test. The minimal amount of the antigen determined by this method is 10 ng/ml.

[Lymphocyte and neutrophil cytochemistry in the dynamics of different forms of diphtheria in adults].

The cytochemical study of lymphocytes and neutrophils isolated from fractionated blood of diphtheria patients and carriers has revealed that a decrease in the activity of lymphocyte succinic dehydrogenase and myeloperoxidase can be observed at all periods of the disease and in all its forms. A decrease in the activity of lymphocyte nonspecific esterase has been noted only in patients with toxic and subtoxic diphtheria and a decrease in the activity of neutrophil alkaline phosphatase, in carriers. The analysis of correlations between the parameters of five enzymes under study (lymphocyte succinic dehydrogenase, lymphocyte acid phosphatase, lymphocyte non-specific esterase, myeloperoxidase, neutrophil alkaline phosphatase) and enzymatic rosette parameters has been made.

[The cellular immune response in different forms of diphtheria in adults].

In 109 adult diphtheria patients with different forms of the disease (toxic, subtoxic, localized) and carriers, the latter including 6 persons having had the localized form of diphtheria, 12 different subpopulations of T and B lymphocytes and neutrophils were studied by the methods of rosette formation with sheep, mouse and bovine red blood cells. The study showed that, starting from week 2 of the disease, an increase in the content of the subpopulations under study was registered in all forms of diphtheria; the disease, as well as in patients with the localized form of the disease accompanied by complications and in long-term carriers. In all forms of diphtheria a decrease in the content of neutrophil rosette-forming cells was shown.