Optimized APEX2 peroxidase-mediated proximity labeling in fast- and slow-growing mycobacteria.

Proximity labeling is a technology for tagging proteins and other biomolecules in living cells. These methods use enzymes that generate reactive species whose properties afford high spatial resolution for the localization of proteins to subcellular compartments and the identification of endogenous interaction partners. Here we present the adaptation of the engineered peroxidase APEX2 to proximity labeling in mycobacteria, including the human pathogen Mycobacterium tuberculosis.

Characteristics of monolayer formation by the chytrid .

is a globally distributed generalist pathogen that has driven many amphibian populations to extinction. The life cycle of has two main cell types, motile zoospores, and sessile reproductive sporangia. When grown in a nutrient-rich liquid medium, forms aggregates of sporangia that transition into monolayers on surfaces and at the air-liquid interface.