Whole-Genome Sequencing of D75 and D76 Confirms Safety and Probiotic Potential.

Whole-genome DNA sequencing of D75 and D76 strains (Vitaflor, Russia) was determined using the PacBio RS II platform, which was followed by de novo assembly with SMRT Portal 2.3.0.

Complete Genome Sequences of the Probiotic Lactic Acid Bacteria D75 and D76.

D75 and D76 were isolated from the intestinal tract of a healthy child. Both strains possess symbiotic, probiotic, and antagonistic activities. We have sequenced and annotated the whole genomes of D75 and D76 and have conducted a preliminary genome comparative analysis.

Low-molecular-weight components of the metabolome control the proliferative activity in cellular and bacterial cultures.

The effects of blood metabolites and model cell culture exometabolites found in the human and animal blood metabolomes have been assessed. Specifically, the influence of carboxylic acids that are structural analogues of amino acids and the drug Aktoflor-C have been studied. Methods of organotypic culturing of rat tissues and Escherichia coli bacterial culturing have been used.

IDENTIFICATION OF BACTERIOCIN GENES IN PROBIOTIC STRAINS OF LACTIC ACID BACTERIA LACTOBACLLUS ACIDOPHILUS D-75 AND LACTOBACILLUS ACIDOPHILUS D-76.

Aim: to detect the bacteriocin genes of probiotic strains Lactobacillus acidophilus 0-75 and Lactobacillus acidophilus D-76 (components of Vitaflor dietary supplement).

Materials And Methods: The antagonistic activity of L. acidophilus D-75 and L.

[Comparative characterization of life quality in patients with pulmonary tuberculosis after surgical treatment].

The parameters of life quality were comparatively analyzed in 59 patients aged 18 to 63 years, operated on for tuberculomas and cavitary masses of the lung in 1995 to 2002. Each patient underwent atypical pneumonectomy in the volume of not more than 2 segments via anterolateral access. Life quality was assessed, by using the Medical Outcomes Study Shirt Form (SF-36) developed by John E.

[Lambda H-lambda T 80 hybrid study of the DNA structural gene region in lambda and phi 80 phages].

Hybrids lambda H lambda T80 are formed due to recombination of the phage lambda att80 and phi 80 prophage partially deleted in the region of structural genes. Genetic structure of 22 independently isolated lambda H lambda T80 hybrids was determined by the restriction method and it was shown that recombination took place in the genes A, C, D and H. The frequencies of hybrid formation diminish from 1.