[Immunologic prognostication of development of recurrences in patients with acute myeloid leukemia].

Morphological study of the bone marrow and blood, cytochemical examination with evaluation of lysosomal enzymes activity, concentrations of glycogen and acid sulfated mucopolysaccharides, and immunophenotyping were conducted to develop immunomorphological criteria early detecting leukemic clone in patients with acute nonlymphoblastic leukemia (ANLL) in remission based on dynamic immunological monitoring of peripheral blood lymphocytes for recurrence prognostication. Immunological monitoring of lymphoid population of ANLL patients' peripheral blood allowed to predict recurrence in remission, several months before its morphological identification in all the patients. This was accomplished by a significant increase in the levels of (M + 3 sigma) lymphocytes with antigens of early differentiation stages atypical for mature lymphocytes.

[Immunomorphocytochemical and immunofluorescent study methods in the immunodiagnosis of acute leukemias].

Immunophenotyping results were compared for blast cells of the bone marrow and blood in a wide panel of monoclonal antibodies to differentiating antigens of the cells in various stages of hemopoiesis. The study was performed in acute leukemia (AL) patients using the immunocytochemical investigation (APAAP) and immunofluorescence (IF). Morphological evaluation of the marrow and blood involved cytochemical tests on glycogen, lipids, acid sulfated mucopolysaccharides, measurements of lysosomal enzymes and myeloperoxidase activities.

[The use of a panel of monoclonal antibodies for immunologic prognosis of recurrence of acute lymphoblastic leukemia].

A method for early immunological prognosis of acute lymphoblastic leukemia (ALL) relapses has been presented. It is based on the dynamic phenotyping of lymphoid blood cells from the patients during remission by the indirect immunofluorescence test using a specially composed panel with monoclonal antibodies (MCA) to differentiation antigens of early lymphopoiesis stages. The panel composed for monitoring the population of leukemic cells during remission, contained 6 MCA selected from 40 MCA samples with the known specificity, basing on the principle of the highest reactivity with lymphoblasts from patients, and minimal positive reactions with lymphocytes from normal subjects.

[Immunologic variants of acute lymphoblastic leukemia in adults and its correlation with cytochemical and various clinical parameters].

The authors provide data pertaining to the immunological phenotyping in the panel of monoclonal antibodies to various differentiating antigens of the leukemic cells in 27 patients with acute lymphoblastic leukemia (ALL). Based on the composition of the differentiating antigens of the leukemic cells the following immunological variants of the disease were identified: pre-B, pre-T and T-ALL--the thymic and mature (activated) ones. The phenotype was compared with the cytochemical parameters of the lymphoblasts of the identified immunological variants of ALL and with some clinical and hematological manifestations of the disease.

[DNA-topoisomerases of animal cells].

The properties and functions of the DNA topoisomerases are reviewed on the basis of the literature and the author's own data. The techniques of isolation and characterization of the covalent complexes of the topoisomerases with DNA are described.

[Isolation and properties of endodeoxyribonuclease from the ovicells of the loach].

A new DNAase was isolated from unfertilized loach eggs. The enzyme is activated by bivalent metal ions (Mg2+, Ca2+, Mn2+) and is inhibited by Na+ and N-ethylmaleimide. The maximal activity of DNAase lies within the pH range of 9.