Development of the Automated Primer Design Workflow Uniqprimer and Diagnostic Primers for the Broad-Host-Range Plant Pathogen .

Uniqprimer, a software pipeline developed in Python, was deployed as a user-friendly internet tool in Rice Galaxy for comparative genome analyses to design primer sets for PCRassays capable of detecting target bacterial taxa. The pipeline was trialed with , a destructive broad-host-range bacterial pathogen found in most potato-growing regions. is a highly variable genus, and some primers available to detect this genus and species exhibit common diagnostic failures.