Evaluation effects of allopurinol and FSH on reduction of ischemia-reperfusion injury and on preservation of follicle after heterotopic auto-transplantation of ovarian tissue in mouse.

Purpose: Allopurinol and FSH injection are applied to reduce ischemia-reperfusion injury and to increase survival rate for ovarian follicles after ovarian heterotopic autotransplantation in mice.

Methods: Ovarian tissues from 6-week-old mice were grafted into back muscle then collected after 3 weeks. A total of five groups were included in this experiment as follows: control group ( = 5), sham-operated group ( = 5), allopurinol treatment group (AP) ( = 5), follicle stimulating hormone (FSH) treatment group ( = 5), as well as, allopurinol and FSH treatment group (APF) ( = 5).