Effect of mixed infection of Mycoplasma gallinarum and Newcastle disease virus (F strain) on the tracheal epithelium of village chickens.

The effects of a mixed infection of Mycoplasma gallinarum and Newcastle disease virus (F strain) on the tracheal epithelium of village chickens were investigated and observed by scanning electron microscopy. Day-old village chicks were vaccinated intranasally with F strain Newcastle disease virus and inoculated intratracheally on the same day with 10(8) colony forming units of M gallinarum. In another study the chicks were vaccinated and then infected with M gallinarum seven days later.

Effect of Mycoplasma gallinarum on embryonated village chicken eggs.

The effect of Mycoplasma gallinarum isolated from village chickens on embryonated village chicken eggs was investigated. Seven-, 10-, 12- and 18-day-old embryos were inoculated with medium containing 10(6) colony forming units of M gallinarum or with uninfected medium and incubated at 37 degrees C until they hatched. There was no significant difference between the infected embryos which failed to hatch and those which hatched or between them and the group inoculated with medium only.

Evaluation of growth of avian mycoplasmas on bile salt agar and in bile broth.

All nine Mycoplasma iowae strains and one strain of M gallinarum grew on 0.05 per cent 'bile' agar medium. The colony size of M iowae on this agar medium was similar to the size obtained on bile-free mycoplasma agar.

Oral challenge of turkey poults with Mycoplasma iowae.

Day-old poults were inoculated orally each day for 7 days with 0.2 ml of Mycoplasma iowae, strain D112, 10(8) colony-forming units/ml. Cloacal swabs were taken from each poult during the inoculation period and at selected intervals until 21 days after the last inoculation.

The viability of Mycoplasma iowae at 40 C in turkey semen.

Mycoplasma iowae was mixed with turkey semen, incubated at 40 C in a water bath, and sampled at different intervals. The M. iowae remained viable throughout a 48-hr incubation period.