Formulation development and evaluation, in silico PBPK modeling and in vivo pharmacodynamic studies of clozapine matrix type transdermal patches.

Clozapine is a potent serotonin receptor antagonist and commonly used for the treatment of Schizophrenia. The study aimed to develop and optimize the transdermal matrix patch of clozapine. A 3-level, 3-factor Central Composite Design was applied to examine and validate the impact of various formulation variables, Eudragit, PEG, and oleic acid on in vitro drug release, flux, and tensile strength (TS).

The Assessment and Efficiency of Cefixime in Upper Respiratory Tract Infections: Insights and Perspectives.

Upper respiratory tract infections (URTIs) are common in patients of the pediatric age group and often lead to significant morbidity and mortality. Antibiotics such as cefixime have contributed to the management of URTIs, particularly when bacterial etiology is suspected. Several studies have evaluated the effectiveness of cefixime in pediatric URTIs, showing promising results in alleviating symptoms and reducing the duration of illness.

Infection prevention and control staffing and programs in Middle Eastern Countries.

Introduction: Infection prevention and control (IPC) programs in the Middle Eastern and North African (MENA) countries are evolving. The objective was to characterize IPC personnel and programs in MENA countries, with special emphasis on the differences between Gulf Cooperation Council (GCC) and non-GCC countries.

Methodology: A cross-sectional online survey was conducted in 2019 among IPC members of the Arab Countries Infection Control Network (AcicN).

3-Methylglutaconic aciduria type VIII in an Indian neonate.

Neonatal encephalopathy manifests with altered sensorium, tone abnormalities, and often with abnormal movements and seizures. The causes are heterogeneous and many. We report a late preterm neonate who presented with depressed sensorium, cranial nerve abnormalities, mixed hypertonia and hypotonia, and respiratory failure.

Antimicrobial activity of different solvent extracted samples from the leaves and fruits of Capsicum annuum.

The current research describes the antimicrobial potential of methanol, n-hexane, n-butanol, ethyl acetate and aqueous extracted samples from the leaves and fruits tissues of Capsicum annuum. Different solvent extracted samples were screened against six pathogenic microorganisms including five bacterial and one fungal specie by disc diffusion susceptibility assay using 1, 2 and 3 mg disc-1 concentrations. When analyzed statistically the data showed that different solvent extracted samples from both leaves and fruits of Capsicum annuum revealed varying degrees of antibacterial and antifungal activities.

Source apportionment of heavy metals in farmland soil with application of APCS-MLR model: A pilot study for restoration of farmland in Shaoxing City Zhejiang, China.

The conditions of the sources of heavy metals are essential to assess its potential threats to human health. The identification of the origin of heavy metals is essential for planning effective measures to control long-term accumulation of heavy metals. In this study, analysis of pollution sources was performed on 100 soil samples with geostatistics and absolute principal component score-multiple linear regression (APCS-MLR) receptor model.

Bioinspired synthesis and characterization of gold nano-particles from medicinally important Periploca hydaspidis and their in vitro antioxidant and antimicrobial activity.

This research investigates the synthesis and characterization of gold nanoparticles from Periploca hydaspidis and their antimicrobial and anti oxidant activity. The synthesis of AuNPs was confirmed by UV-Vis spectrophotometer and structure by a high resolution atomic force microscope. X-ray diffraction and Fourier transformed infrared spectroscopy was used to study the crystallite size and different functional groups.

Bioavailability of heavy metals in contaminated soil as affected by different mass ratios of biochars.

The biochars are efficient adsorbent of heavy metals. However, the type of biochar species determines the effectiveness of biochars for immobilization of heavy metals. In this experiment, we mixed different biochars in variable mass ratios and evaluated their effects on plant growth and bioavailability of heavy metals.

Effect of different solvent extracted samples from the leaves and fruits of Datura stramonium on the growth of bacteria and fungi.

Different solvent extracted samples from the leaves and fruits tissues of D. stramonium were tested against five pathogenic microorganisms by disc diffusion susceptibility method using 1, 2 and 3mg disc concentrations. Methanol and chloroform extracted fractions from both leaves and fruits measured good growth inhibition of all the tested microorganisms at all concentrations.

Effects of biochar on growth, and heavy metals accumulation of moso bamboo (Phyllostachy pubescens), soil physical properties, and heavy metals solubility in soil.

Pot experiment was conducted to investigate the effects of wood biochar (5%), bamboo biochar (5%), rice straw biochar (5%) and Chinese walnut shell biochar (5%) on growth, accumulation of heavy metals in moso bamboo, soil physical properties, and solubility of heavy metals in soil. The results revealed that dry weight of moso bamboo was significantly increased in treatments of wood biochar (5%), rice straw biochar (5%) and Chinese walnut shell biochar (5%) except bamboo biochar (5%). Application of straw biochar (5%) was most effective in enhancing plants biomass, with increase of 157%, 113% and 111% in leaves, roots and stems of moso bamboo.

Extraction and isolation of important bioactive compounds from the fruit of Physalis ixocarpa.

The current study investigates pharmaceutically important bioactive compounds in the fruits of Physalis ixocarpa. Two different extractions methods were used to study its effect on percent extract yield, recovery of bioactive compounds and antioxidant activity of the extracts. The data indicated that Soxhlet extraction had high efficiency of recovery than maceration method for extracting compounds; percent extract yield and antioxidant activity of the extracts.

GC-MS profile of bioactive compounds from medicinally important Periploca hydaspidis.

The aim of the present study was to investigate bioactive compounds in different solvent extracted samples from the stem tissues of P. hydaspidis using Gas Chromatography and Mass Spectroscopy techniques. GC-mass spectrum was compared with the data base of National Institute of Standards and Technology (NIST) containing more than 62000 patterns of the mass spectrum.

Impact of different solvent extracts from leaves and fruits of Eucalyptus globulus on growth of different bacteria and fungi.

The present research investigates the antimicrobial activities of the samples extracted from the leaves and fruits of Eucalyptus globulus through disc diffusion susceptibility assay using 1, 2 and 3mg disc-1 concentrations. Different extracted samples from the leaves and fruits of Eucalyptus globulus exhibited different degrees of antimicrobial. The data indicated that n-butanol and ethyl acetate extracted fraction of both the leaves and fruits inhibited the growth of all microorganisms at all the tested concentrations.

Effect of amendments on contaminated soil of multiple heavy metals and accumulation of heavy metals in plants.

The contamination of soil with heavy metals is a severe problem due to adverse impact of heavy metals on environmental safety and human health. It is essential to remediate soil contaminated with heavy metals. This study has evaluated the effects of pine biochar, kaolin, and triple super phosphate (TSP) on multiple heavy metals (Ni, Zn, Cu, and Cd) in contaminated soil and accumulation of heavy metals in plants.

Antinociceptive, antimicrobial potential and phytochemical screening of different solvent extracted samples from the stem of Acer pentapomicum.

The present study investigates the antinociceptive, antimicrobial activity and phytochemical assessment of samples from A. pentapomicum. Different microbes were tested using disc diffusion assay at three concentrations (1, 2 and 3 mg/disc).

Phytochemical analysis, phytotoxic and insecticidal activities of medicinally important Periploca hydaspidis.

The present study investigates the phyto-chemical analysis, phytotoxic activity and insecticidal activity of the stem and root tissues of medicinally important Periploca hydaspidis. All the extracted samples exhibited the phytotoxic activity. The data confirmed that water was the best solvent to extract the phytotoxic compounds from stem and root tissues.

Effect of EDTA and citric acid on absorption of heavy metals and growth of Moso bamboo.

The effect of EDTA and citric acid on accumulation, toxicity of heavy metals (Cu, Zn, Cd, and Pb), and growth of Moso bamboo was investigated in current experiment. The availability of heavy metals in soil and its uptake by plants has indicated toxicity. The results revealed that EDTA and citric acid has reduced biomass of Moso bamboo but non-significant difference in biomass was observed compared with control.

Postgraduate Medical Trainees Understanding of Biostatistics: A Pre- and Post-Research Methodology Workshop Experience.

Objective: To evaluate understanding of biostatistics among postgraduate medical trainees before and after biostatistics workshop.

Study Design: Quasi experimental study.

Place And Duration Of Study: Regional Centre, Islamabad, College of Physicians and Surgeons Pakistan, from March to September 2017.

Phytochemical screening and antibacterial activity of different solvent extracted samples of Arisaema jacquemontii.

The current research was carried out to assess the antibacterial activities and phytochemical analysis of the methanol, n-hexane, ethyl acetate, n-butanol soluble fractions and aqueous extracts of the tubers of Arisaema jacquemontii. All the extracts were tested for their antibacterial potential at 1, 2 and 3 mg disc concentrations against 6 bacterial strains through disc diffusion suseptibility assay. The data suggested that different extracts showed varying degree of growth inhibition against the tested microbes.

Report - Screening of medicinally important Berberis lyceum for their antimicrobial activity by disc diffusion assay.

The present research was focused on the anti-microbial activities of different solvent extracted fractions from commercial available and fresh plants of Berberis lyceum against Gram positive, Gram-negative bacteria and fungi using 1 and 2 mg disc concentrations. Our results showed that fractions from both sources revealed different degree of antimicrobial activities. Our result indicated that Escherichia coli, Citorbacter freundii and Candida albicans were more susceptible to crude methanolic extract and the same microbes were resistant to water extracted fractions.

Fractionation of crude extracts from controlled dried and commercially available stem bark of Juglans regia and their antimicrobial effects.

The current research investigates the anti-microbial activities of methanol, ethyl acetate, n-hexane , n-butanol and water extracted samples from controlled dried and commercial bark of walnut (Juglans regia) against five bacterial (Staphylococcus aureus, Bacilus subtilis, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia and one fungal pathogenic specie (Candida albicans) by discs diffusion susceptibility assay using 0.5 and 1mg disc- concentrations. Our results revealed that all the extracts from controlled dried and commercial bark of walnut showed varying degrees of antimicrobial activities.

In vitro antibacterial and antifungal activities of different solvent extracted samples from the stem of Euphorbia heliscopia.

This paper presents the anti-microbial potentials of methanol, ethyl acetate, n-hexane, butanol and water extracted samples from the stem of Euphorbia heliscopia against S. aureus (Gram positive), B. subtilis (Gram positive), P.

Screening of leaves extracts from Calamus aromaticus for their antimicrobial activity by disc diffusion assay.

The current research investigates the anti-microbial activities of different solvent extracted samples from the leaves of Calamus aromaticus against Gram positive, Gram negative bacteria and fungi using 500, 1000 and 2000 μg disc concentrations. Escherichia coli, Citorbacter freundii and Candida albicans showed resistivity to crude methanolic extract and the same microbes were more susceptible to water extracted fractions. Maximum activity was measured by hexane extracted fractions against Pseudomonas aeruginosa, Bacillus subtilis and Xanthomonas campestris and minimum growth inhibition by water extracted fractions.

Effect of lead (Pb) on antioxidation system and accumulation ability of Moso bamboo (Phyllostachys pubescens).

The antioxidation system and accumulation ability of Moso bamboo (Phyllostachys pubescens), which is a valuable remediation material with large biomass and rapid growth rate were studied in hydroponics and pot experiments. In hydroponics experiment, TBARS concentrations and SOD activities decreased with increase of Pb treatments. The activities of POD boost up with elevated Pb treatments, and reached peak level with application of 400μM Pb.