Role of Orphan ParA Proteins in Replication and Cell Division in Rhodococcus erythropolis PR4.

Bacteria have a very well-regulated mechanism for chromosome segregation and cell division. This process requires a large number of complex proteins to participate and mediate their functionality. Among these complex proteins, ParA and ParB play a vital role for the faithful segregation of chromosome.

Genomic deletions in based on transformation of linear heterologous DNA.

Several genome engineering methods have been developed for . However, they suffer from limitations such as extensive cloning, multiple steps, successful expression of heterologous genes via plasmid etc. Here, we report a rapid method for performing genomic deletions/disruptions in spp.