Rigorous electromagnetic theory for waveguide evanescent field fluorescence microscopy.

Recently, waveguide evanescent field fluorescence (WEFF) microscopy was introduced and used to image and analyze cell-substrate contacts. Here, we establish a comprehensive electromagnetic theory in a seven-layer structure as a model for a typical waveguide-cell structure appropriate for WEFF microscopy and apply it to quantify cell-waveguide separation distances. First, electromagnetic fields at the various layers of a model waveguide-cell system are derived.