A Novel Digital Score for Abundance of Tumour Infiltrating Lymphocytes Predicts Disease Free Survival in Oral Squamous Cell Carcinoma.

Oral squamous cell carcinoma (OSCC) is the most common type of head and neck (H&N) cancers with an increasing worldwide incidence and a worsening prognosis. The abundance of tumour infiltrating lymphocytes (TILs) has been shown to be a key prognostic indicator in a range of cancers with emerging evidence of its role in OSCC progression and treatment response. However, the current methods of TIL analysis are subjective and open to variability in interpretation.

Characterization and in vivo study of decellularized aortic scaffolds using closed sonication system.

Extracellular matrix (ECM) based bioscaffolds prepared by decellularization has increasingly emerged in tissue engineering application because it has structural, biochemical, and biomechanical cues that have dramatic effects upon cell behaviors. Therefore, we developed a closed sonication decellularization system to prepare ideal bioscaffolds with minimal adverse effects on the ECM. The decellularization was achieved at 170 kHz of ultrasound frequency in 0.

Enhanced photoelectrochemical water splitting activity of carbon nanotubes@TiO nanoribbons in different electrolytes.

Hydrogen production from water splitting by a photocatalytic process is one way that can be used to solve global problems related to energy depletion and environmental pollution. This work aims to design and characterize a novel photocatalyst nanohybrid carbon nanotubes@TiO nanoribbons (CNTs@TNRs) for enhanced photoelectrochemical (PEC) water splitting in different electrolytes under visible light irradiance. Here, hydrothermal and chemical vapor deposition (HT-CVD) were combined to grow CNTs @ the nanopits of TNRs producing network of nanohybrid CNTs@TNRs.

Development of decellularized meniscus using closed sonication treatment system: potential scaffolds for orthopedics tissue engineering applications.

Purpose: Meniscus is a fibrocartilagenous tissue that cannot effectively heal due to its complex structure and presence of avascular zone. Thus, tissue engineering and regenerative medicine offer an alternative for the regeneration of meniscus tissues using bioscaffolds as a replacement for the damaged one. The aim of this study was to prepare an ideal meniscus bioscaffold with minimal adverse effect on extracellular matrix components (ECMs) using a sonication treatment system.

Ni-doped and Ni/Cr co-doped TiO nanotubes for enhancement of photocatalytic degradation of methylene blue.

Ni-doped and Ni/Cr co-doped TiO nanotubes were successfully synthesized using a novel hydrothermal method. The surface and bulk properties of as-synthesized nanopowders were characterized using various microstructural and optical techniques. The photocatalytic ability of these nanopowders was investigated systematically for the decomposition of methylene blue dye (MB) under visible light illumination.

Cyclic voltammetry growth and characterization of Sn-Ag alloys of different nanomorphologies and compositions for efficient hydrogen evolution in alkaline solutions.

Electrodeposition of silver, tin and their alloys from different aqueous electrolytes suffer from various environmental issues and deposits are affected by H evolution and metal oxide formation. In this work, these metals and their alloys are electrodeposited by cyclic voltammetry utilizing ionic liquids based on CHClNO (choline chloride) that permits deposit properties control. Both Ag and Sn concentrations in the ionic liquid were varied from 0.

Relation Between Immunohistochemical Expression of Hippo Pathway Effectors and Chronic Hepatitis Induced Fibrosis in Egyptian Patients.

Objective: Chronic hepatitis is a global health problem especially in Egypt. Hepatic fibrosis is a common end clinical manifestation of many chronic liver diseases. Although it is a wound-healing process, excessive accumulation of fibrillary collagen leads to architectural damage, cirrhosis and liver failure.

Mechanisms of tilt-induced vasovagal syncope in healthy volunteers and postural tachycardia syndrome patients without past history of syncope.

Upright tilt table testing has been used to test for vasovagal syncope (VVS) but can result in "false positives" in which tilt-induced fainting (tilt+) occurs in the absence of real-world fainting. Tilt+ occurs in healthy volunteers and in patients with postural tachycardia syndrome (POTS) and show enhanced susceptibility to orthostatic hypotension. We hypothesized that the mechanisms for hypotensive susceptibility differs between tilt+ healthy volunteers (Control-Faint (N = 12)), tilt+ POTS patients (POTS-Faint (N = 12)) and a non-fainter control group of (Control-noFaint) (N = 10).

Matrix Production in Chondrocytes Transfected with Sex Determining Region Y-Box 9 and Telomerase Reverse Transcriptase Genes: An Evaluation from Monolayer Culture to Three-Dimensional Culture.

Background: This study aimed to observe the cartilaginous matrix production in SRY (sex determining region Y)-box 9 ()- and/or telomerase reverse transcriptase ()-transfected chondrocytes from monolayer to three-dimensional (3D) culture.

Methods: The genes were transferred into chondrocytes at passage-1 (P1) via lipofection. The post-transfected chondrocytes (-, - and /) were analysed at P1, P2 and P3.

A case report of an asymptomatic male child with multiple entero-enteric fistulae post multiple magnet ingestion.

Introduction: Foreign body ingestion cases are very common in children. They usually present with obvious symptoms allowing proper diagnosis and management; however, magnet ingestion can be asymptomatic. This disorder is considered uncommon as well as challenging to diagnose before complications of intestinal obstruction or peritonitis occur due to entrapment of intestinal walls in the magnetic field.

Methods for Segmentation and Classification of Digital Microscopy Tissue Images.

High-resolution microscopy images of tissue specimens provide detailed information about the morphology of normal and diseased tissue. Image analysis of tissue morphology can help cancer researchers develop a better understanding of cancer biology. Segmentation of nuclei and classification of tissue images are two common tasks in tissue image analysis.

Perineal massage and training reduce perineal trauma in pregnant women older than 35 years: a randomized controlled trial.

Introduction And Hypothesis: The aim of this study was to evaluate the effectiveness of perineal massage, pelvic floor muscle training (PFMT) and a pelvic floor dysfunction (PFD) prevention educational program in pregnant women above the age of 35 years to prevent perineal tear and episiotomy.

Methods: A randomized parallel assignment study involved two groups of pregnant women at the obstetrics outpatient clinic 4 weeks prior to their due date. The first group (n = 200) was educated to do digital perineal massage and pelvic floor muscle training and received an educational PFD prevention program.

Heart Disease in Adult Syrian Refugees: Experience at Jordan University Hospital.

Background: Since 2011, 1.26 million Syrians have immigrated to Jordan, increasing demands on Healthcare service. Information about cardiovascular disease (CVD) in Syrian refugees in general, and specifically in Jordan, is unknown.

Genome-Wide Identification of Papain-Like Cysteine Proteases in and Functional Characterization in Response to .

Cotton, a natural fiber producing crop of huge importance, is often prone to attack of . Papain-like cysteine proteases (PLCPs) constitute a large family in plants and were proposed to involve in plant defense against pathogen attack in a number of studies. However, there is no detailed characterization of genes in cotton against infection of .

Effect of Cu, Ni and Pb doping on the photo-electrochemical activity of ZnO thin films.

In the present study, the effects of metallic doping on the photoelectron\chemical properties of zinc oxide thin films have been studied. All films have been deposited using the spray pyrolysis technique at a constant doping level of 3 wt% whereby Cu, Ni, and Pb were used as dopants. The structure of all films was studied by X-ray diffraction which showed the grain size of all doped films to be 50 nm.

Developing Egyptian water quality index for drainage water reuse in agriculture.

In Egypt, water quality (WQ) is an ordinary activity. However, the calculation of a tailor-made Egyptian WQ index is not. Therefore, this research attempts to develop an index for the agricultural drainage water (EG-DWQI) that can be reused for irrigation based on collected WQ data measured monthly from August 2000 to July 2015.

Suppression of tryptophan synthase activates cotton immunity by triggering cell death via promoting SA synthesis.

Primary metabolism plays an important role in plant growth and development, however the relationship between primary metabolism and the adaptive immune response is largely unknown. Here, we employed RNA interference (RNAi), virus-induced gene silencing (VIGS) technology, phytohormone profiling, genetic studies, and transcriptome and metabolome analysis to investigate the function of the tryptophan synthesis pathway in the resistance of cotton to V. dahliae.

Micro-Net: A unified model for segmentation of various objects in microscopy images.

Object segmentation and structure localization are important steps in automated image analysis pipelines for microscopy images. We present a convolution neural network (CNN) based deep learning architecture for segmentation of objects in microscopy images. The proposed network can be used to segment cells, nuclei and glands in fluorescence microscopy and histology images after slight tuning of input parameters.

The cytochrome P450 of Bacillus megaterium A14K is induced by 2,3,7,8-Tetrachlorinated dibenzo-p-dioxin: Biophysical, molecular and biochemical determinants.

The current study describes biological changes in Bacillus megaterium A14K cells growing in the presence of 2,3,7,8-Tetrachlorinated dibenzo-p-dioxin (TCDD), the most potent congener of dioxins. The results indicate that the metabolizing of 2,3,7,8-TCDD by BmA14K was accompanied with a novel morphological and biophysical profile typified by the growth of single cells with high levels of biosurfactant production, surface hydrophobicity and cell membrane permeability. Moreover, the TCDD-grown bacteria exhibited a specific fatty acid profile characterized by low ratios of branched/straight chain fatty acids (BCFAs/SCFAs) and saturated/unsaturated fatty acids (SFAs/USFAs) with a specific "signature" due to the presence of branched chain unsaturated fatty acids (BCUFAs).

Phytohormones enhanced drought tolerance in plants: a coping strategy.

Drought stress is a severe environmental constraint among the emerging problems. Plants are highly vulnerable to drought stress and a severe decrease in yield was recorded in the last few decades. So, it is highly desirable to understand the mechanism of drought tolerance in plants and consequently enhance the tolerance against drought stress.

Efficient photocatalytic removal of safarnin-O dye pollutants from water under sunlight using synthetic bentonite/polyaniline@NiO photocatalyst of enhanced properties.

This study involves a synthesis of bentonite/polyaniline composite (BE/PANI) of enhanced physicochemical properties as catalyst support for NiO photocatalyst. The change in the structural properties, morphological features, and optical behavior was addressed utilizing several analytic techniques. The characterization results reflected considerable enhancement in the specific surface area after the integration between bentonite and polyaniline (127 m/g) and after loading of the campsite by NiO forming bentonite/polyaniline@NiO composite (BE/PANI@NiO) (231 m/g).

Microbiome Diversity in Cotton Rhizosphere Under Normal and Drought Conditions.

Climate change contributes to drought stress and subsequently affects crop growth, development, and yield. The microbial community, such as fungi and bacteria in the rhizosphere, is of special importance to plant productivity. In this study, soil collected from a cotton research field was used to grow cotton plants (Gossypium hirsutum cv.

Identification of a dioxin-responsive oxylipin signature in roots of date palm: involvement of a 9-hydroperoxide fatty acid reductase, caleosin/peroxygenase PdPXG2.

Dioxins are highly hazardous pollutants that have well characterized impacts on both animal and human health. However, the biological effects of dioxins on plants have yet to be described in detail. Here we describe a dioxin-inducible caleosin/peroxygenase isoform, PdPXG2, that is mainly expressed in the apical zone of date palm roots and specifically reduces 9-hydroperoxide fatty acids.

GhUMC1, a blue copper-binding protein, regulates lignin synthesis and cotton immune response.

Verticillium wilt caused by the soil-borne fungus Verticillium dahliae is a serious problem for the sustainable production of cotton. The mechanism of cotton resistance to V. dahliae is unclear, which makes it is difficult to improve cotton resistance breeding.

Hemodynamic characteristics of postural hyperventilation: POTS with hyperventilation versus panic versus voluntary hyperventilation.

Upright hyperventilation occurs in ~25% of our patients with postural tachycardia syndrome (POTS). Poikilocapnic hyperventilation alone causes tachycardia. Here, we examined changes in respiration and hemodynamics comprising cardiac output (CO), systemic vascular resistance (SVR), and blood pressure (BP) measured during head-up tilt (HUT) in three groups: patients with POTS and hyperventilation (POTS-HV), patients with panic disorder who hyperventilate (Panic), and healthy controls performing voluntary upright hyperpnea (Voluntary-HV).