Repurposing pinaverium bromide against Staphylococcus and its biofilms with new mechanisms.

Antibiotic resistance by methicillin-resistant Staphylococcus aureus (MRSA) is an urgent threat to human health. The biofilm and persister cells formation ability of MRSA and Staphylococcus epidermidis often companied with extremely high antimicrobial resistance. Pinaverium bromide (PVB) is an antispasmodic compound mainly used for irritable bowel syndrome.

Identifying potential drug targets for myocardial infarction through Mendelian randomization.

Background: This study explored the associations between plasma and cerebrospinal fluid (CSF) proteins and myocardial infarction (MI) risk. Identifying specific proteins as biomarkers for MI could enhance our understanding of disease mechanisms and inform clinical practice.

Methods: We combined protein quantitative trait loci (pQTL) data for plasma and CSF proteins with genome-wide association study (GWAS) summary statistics for MI.

Effects of Surfactants on Oil Droplet Demulsification in Oil-in-Water Emulsions under an Electric Field: A Molecular Dynamics Study.

The application of an electric field to demulsification of oil-in-water (O/W) emulsions has received extensive attention. However, microcosmic information about the effect of surfactant type on the demulsification of an O/W emulsion under an electric field is still rare. In this work, the effects of cationic surfactant cetyltrimethylammonium bromide (CTAB) and anionic surfactant sodium dodecyl sulfate (SDS) on oil droplet demulsification in an emulsion under a pulsed-DC electric field were studied by molecular dynamics (MD) simulation.

Insights into the source characterization, risk assessment and ozone formation sensitivity of ambient VOCs at an urban site in the Fenwei Plain, China.

The ground-level O concentration has shown a deteriorating trend in the Fenwei Plain of China, which poses a greater challenge for formulating control strategies of O precursor (VOCs). To accurately control VOCs sources and effectively reduce O concentration from a seasonal perspective, online monitoring of 114 VOCs was conducted at Yuncheng Middle School Station from January 1, 2021 to December 31, 2021. The VOCs concentration showed a seasonal variation with the highest in winter and the lowest in summer.

Association between admission hyperglycaemia with in-hospital mortality rate in patients with hypertension and acute aortic dissection.

Objective: Admission hyperglycaemia is recognized as a contributor to poor outcomes in patients with cardiovascular and cerebrovascular diseases. This study aimed to evaluate the association between admission hyperglycaemia and in-hospital mortality in patients with hypertension and acute aortic dissection (AAD).

Methods: Patients diagnosed with hypertension and AAD between 1 January 2010 and 1 March 2023 were enrolled into this retrospective observational study, and divided into two groups based on admission blood glucose levels (BGA): normoglycemia (BGA < 7.

Increase of ATP synthesis and amino acids absorption contributes to cold adaptation in Antarctic bacterium Poseidonibacter antarcticus SM1702.

Numerous psychrophiles inhabit the cold environments that are prevalent across the global biosphere. The adaptation of psychrophiles to cold conditions has been widely studied in strains from the archaeal phylum Euryarchaeota and the bacterial class Gamma-proteobacteria. However, given the vast diversity of microorganisms in cold environments, many microbial lineages with potentially unique cold-adaptation strategies remain largely unexplored.

Temperature-Programmed Desorption of Single Zeolite Nanoparticles.

Zeolites are essential solid acid catalysts in various chemical processes. Temperature-programmed desorption (TPD) is one of the most established techniques used to characterize the acidity of zeolites by measuring the desorption kinetics of probes from bulk samples. However, conventional TPD can hardly deliver the intrinsic acid properties of zeolites because the apparent desorption kinetics are inevitably mixed with mass transfer and thermal conduction due to the large sample amount (∼0.

A facile optical sensing strategy for glyphosate detection based on structure-switching signaling aptamers.

A facile and highly specific optical sensing strategy is established for glyphosate (GLYP) detection using structure-switching signaling aptamers (F-SSSAs) with fluorescence signal reporting functionality. The strategy involves two domains: the FITC-labeled signal transduction domain for fluorescence signal reporting, while the functional domain (specific structure-switching aptamers) controls the target recognition. Graphene oxide (GO) works as a robust F-SSSAs quencher in the absence of GLYP.

Structure-based discovery of a new type of scaffold compound as binding competitors for protein-bound Uremic Toxins.

Protein-bound uremic toxins (PBUTs) are the main cause of uremia, but traditional hemodialysis is ineffective in removing them because of their strong ability to bind to human serum albumin (HSA), highlighting the need for new treatments. In this study, first, structure-based docking was used to screen a diverse library of 200,376 virtual compounds against the active sites I and II. After two rounds of docking screening, 3944 candidate molecules were obtained.

FGFR2 fusions assessed by NGS, FISH, and immunohistochemistry in intrahepatic cholangiocarcinoma.

Background: FGFR2 fusion has become a promising therapeutic target in iCCAs; however, the procedure for screening FGFR2 fusion has not been conventionally developed.

Methods: FGFR2 fusion was identified using DNA + RNA-based NGS and FISH, and the concordance between DNA + RNA-based NGS, FISH, and IHC was compared.

Results: FGFR2 fusions were detected in 9 out of 76 iCCAs (11.

Optical imaging of the stochastic nucleation kinetics and intrinsic activation energy of single spin-crossover nanoparticles.

Cooperative spin crossover (SCO) compounds are one of the most promising molecular bistable solids due to their intriguing thermal hysteresis phenomena around room temperature. It is well known that hysteresis is an essential kinetic effect, however, accurate assessment of the spin transition kinetics of SCO nanomaterials remains scarce. Herein, we developed a thermal-optical methodology to image the thermally induced spin transition kinetics of single SCO nanoparticles in a quantitative, repeatable, and high-throughput manner.

Dihydromyricetin treats pulmonary hypertension by modulating CKLF1/CCR5 axis-induced pulmonary vascular cell pyroptosis.

Pulmonary hypertension (PH) is a progressive cardiopulmonary disease characterized by elevated pulmonary artery pressure and vascular remodeling, resulting in poor prognosis and increased mortality rates. Chemokine-like factor 1 (CKLF1) plays a significant role in inducing inflammation and cell proliferation, both of which are critical processes in the pathogenesis of various diseases. Dihydromyricetin (DMY) has garnered attention for its potent anti-inflammatory properties.

Molecular insight into algae-derived dissolved organic matters via Fourier-transform ion cyclotron resonance mass spectrometry: Effects of pretreatment methods and electrospray ionization modes.

The release of algae-derived dissolved organic matter (ADOM) significantly increased in serious eutrophication waters, posing great threats to drinking water safety. Thus, the molecular composition decipherment is urgently in need. However, due to unsatisfactory pretreatment and ionization effects, the application of Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) on ADOM was limited.

Conformational and Solvent Effects on the Photoinduced Electron Transfer Dynamics of a Zinc Phthalocyanine-Benzoperylenetriimide Conjugate: A Nonadiabatic Dynamics Simulation.

Herein, we employed a combination of static electronic structure calculations and nonadiabatic dynamics simulations at linear-response time dependent density functional theory (LR-TDDFT) level with the optimally tuned range-separated hybrid (OT-RSH) functional to explore the ultrafast photoinduced dynamics of a zinc phthalocyanine-benzoperylenetriimide (ZnPc-BPTI) conjugate. Due to the flexibility of the linker, we identified two major conformations: the stacked conformation (ZnPc-BPTI-1) and the extended conformation (ZnPc-BPTI-2). Since the charge transfer states are much lower than the lowest local excitation in ZnPc-BPTI-1, which is contrary to ZnPc-BPTI-2, the ultrafast electron transfer (~3.

Long-Term Prognosis and Influencing Factors of Chinese Adolescents With Temporomandibular Disorder After Physical Therapy.

Background: Physical therapy is the preferred conservative treatment for patients with temporomandibular disorder (TMD). However, few studies have investigated the application of physical therapy in adolescents, especially follow-up studies on the long-term prognosis of these patients. This study investigated the short-term effects and long-term prognosis of physical therapy in adolescent patients with TMD and the factors influencing long-term symptoms.

Clinical efficacy evaluation of the implantation of a diffractive trifocal intraocular lens in high myopia eyes: Two-year follow-up.

Purpose: To evaluate the long-term subjective and objective visual quality after implantation of a diffractive trifocal intraocular lens (IOL) in high myopic eyes.

Methods: A total of 53 eyes from 38 patients with high myopia who underwent phacoemulsification and trifocal IOL implantation were investigated. The visual acuity, contrast sensitivity, diopter, defocus curve, and objective visual quality at 1 month, 3 months, 1 year, and 2 years after surgery, as well as the satisfaction questionnaire and VF-14 scale, were statistically analyzed.

Association between stress hyperglycemia ratio and all-cause mortality in critically ill patients with atrial fibrillation: insights from a MIMIC-IV study.

Background: The stress hyperglycemia ratio (SHR) has emerged as a potential prognostic indicator for various critical illnesses. However, its role in determining outcomes in patients with atrial fibrillation (AF) within the intensive care unit (ICU) remains unclear. This study aimed to elucidate the association between SHR and all-cause mortality in this clinical setting.

Physical therapy and non-surgical manual disc reduction combined with anterior repositioning splint for acute disc displacement without reduction of the temporomandibular joint in adolescents.

Objectives: Current studies on the treatment of adolescent patients with disc displacement without reduction (DDWOR) are limited by short follow-up periods and small sample sizes, and there are few comparative studies on the efficacy of conservative treatment with and without disc reduction for acute DDWOR. This study compared the therapeutic effects of two conservative treatment methods: physical therapy alone and physical therapy combined with non-surgical manual disc reduction and anterior repositioning splints (ARS), in adolescent patients with acute DDWOR.

Materials And Methods: This retrospective study included adolescent patients with DDWOR who underwent physical therapy at the Temporomandibular Joint Rehabilitation Department of the Shanghai Ninth People's Hospital from January 2018 to December 2021.