[Estimation of effectiveness of peroral mucolytic agents in treatment of inflammatory diseases of middle ear and paranasal sinusitis].

The aim of the work was estimation of perolar mucolytic agents in treatment of inflammatory diseases of middle ear and paranasal sinusitis. On the basis of research it was concluded that Sinupret reduced the necessity of grommet. Sinupret is the best systemic mucolytic in the treatment of inflammatory disease of middle ear and paranasal sinuses.

[The role of oxidative metabolism in pathogenesis of hyperbilirubinemia in infants].

The aim of the study was the investigation of free radical oxidation intensity in blood of newborn infants with hyperbilirubinemia. In infants blood the bilirubin, free nitric oxide, lipoperoxide radicals Fe(2+)-ions, methemoglobin, oxy- and desoxy hemoglobin content, erythrocytes deformability and resistance were studied. Results of the study testify the intensive generation of free nitric oxide, release of ferritin-free ferrous (Fe(2+)), intensification of lipoperoxidation, decrease of red blood cells resistance, and accumulation of methemoglobin in blood of newborn infants with hyperbilirubinemia.

[Surgical treatment of otitis media purulenta chronica].

Evolution of views on surgical treatment of otitis media purulenta chronica for the last decade and outcomes of this surgery in the last 5 years are reviewed. The authors made tympanoplastic operations in 220 patients. Function of the tympanic membrane recovered in 212 cases, a complete closure of the bone-air gap was observed in 89 patients; hearing improvement averaged 25 dB.

Histological and histochemical investigations of nasal septum cartilage in nasal deformations.

Unlabelled: AIM of the study is to identify histological and histochemical peculiarities of nasal septum cartilage reconstruction in patient with different remoteness of trauma.

Materials And Methods: Patients of both sexes at the age of 16-39 years have been operated on. Depending on remoteness of the period from the trauma, witch caused the deformation, till the surgery, the materials was divided into 2 group: I group (36 patients) 1-5 years from the trauma to the surgery, II group (23 patient) 10-20 years from the trauma to the surgery.

[Heat capacity of native DNA fibers at 2-25 K].

The data on low-temperature heat capacity (2-25 K) of native DNA strands at different humidities are presented, which take into account the specificity of hydration of DNA due to its chemical composition (GC-content). The temperature dependence of DNA heat capacity (Cp = f(T)) at 2-4 K was analyzed. Analysis of Cp = f(T) involves both the ordinary Debay density and excessive low-energy density of oscillatory states (DOS) peculiar to noncrystalline solids.

[The effect of Cu2+, Zn2+ cations and biogenic amines on the nuclear poly(ADP-ribose) polymerase activity in the rat brain].

Study of the effects of Cu2+, Zn2+ cations and polyamines, spermine and spermidine, on the nuclear poly(ADP-ribose)polymerase activity of rat brain was carried out. It was shown that low concentrations of Cu2+ stimulate the activity of purified poly(ADP-ribose)polymerase. The poly(ADP-ribose)polymerase activity was increased 1.

[The effect of the x-ray irradiation of rats on the NAD-pyrophosphorylase and poly(ADP-ribose)polymerase activities of brain nuclei and on the NAD content in nerve tissue].

A study was made of the influence of X-irradiation of rats with various doses on NAD-pyrophosphorylase and poly(ADP-ribose) polymerase activity of brain nuclei. It was shown that X-radiation was ineffective with regard to NAD-pyrophosphorylase activity of nuclei and increased their poly(ADP-ribose) polymerase activity. Stimulation of poly(ADP-ribose) polymerase activity of nuclei was a function of radiation dose and correlated with the decrease in the NAD content of nervous tissue.

[Study of nuclear poly(ADP-ribose)polymerase and DNA-topoisomerase II of brain cells during postnatal development of rats].

The nuclear poly(ADP-ribose)polymerase activity of neuronal and glial cells during postnatal development of rats was studied. It was shown that the poly(ADP-ribose)polymerase activity of nuclei and nuclear matrix of neuronal cells during postnatal development of rats is increased, whereas the polymerase activity of glial cell nuclei and nuclear matrix in newborn and adult rats is higher than in 14-day-old animals. The DNA-topoisomerase II activity of neuronal nuclear matrix during the postnatal development of rats does not change, whereas the topoisomerase activity of glial nuclear matrix decreases but is always higher than the DNA-topoisomerase II activity of neuronal cell matrix during the postnatal development of rats.

[Low-temperature heat capacity of DNA in various conformation states].

Experimental results are presented on temperature--dependent DNA heat capacity in three different states: a) intact--native DNA in the conformation of double helix, b) disordered conformation of polynucleotide chains in the state of statistical coils, c) completely "degenerated" polynucleotide chains--mechanical mixture of nucleotides. Data on heat capacity (4-400 K) at different water content in the specimens allow a definition of relative changes in the pattern of the entropy temperature dependence for these conformational states with the account for the structural effect of water.

[Heat-capacity of DNA at low temperatures and varying humidities].

Adiabatic low temperature microcalorimeter working in a wide temperature range, including that of liquid helium, is described. Heat capacity of DNA at various water concentrations was measured at 4-400 degrees K. It is shown that the pattern of its change is determined by the hydration parameter n (number of water molecules per mole of base pairs).

[Investigation of hydration of poly[d(A-T)] polynucleotide in helical and random coli states by the method of low temperature scanning microcalorimetry].

Thermodynamic parameters of the process of water phase transition in poly[d(A-T)] solutions in the wide range of polymer concentration have been studied. Curves of the heat capacity changes (delta Cp) depending on concentration of polymer and the temperature in the area of ice-water phase transition have been plotted. Thermodynamic parameters of the process of helix--random coli transition in water solutions of poly[d(A-T)] have been measured.

[Ribonuclease hydration and its heat stability in solutions of H2O and D2O].

Ribonuclease hydration in wide concentration and temperature range using differential scanning microcalorimetry and NMR technique was studied. The temperature- concentration equilibrium diagram for H2O--ribonuclease system was suggested. Protein hydration in D2O was shown to be higher than in ordinary water.

[Microcalorimetric study of thermodynamic values of aqueous phase transition and eutectic melting in the binary and ternary systems Na-DNA--H20 and Na-DNA--H20--NaCl].

Thermodynamic parameters of water phase transition and eutectics melting in DNA water-salt solution were studied by means of low temperature scanning differential microcalorimetry at different concentrations of polyanions. DNA hydration was measured and the character of changes in Na+ counterion binding was revealed during the transition of double helix into the state of random coils.