Thermodynamic properties of blood plasma of patient suffering from atherosclerosis.

The goal of the work is to find out the changes in the heat parameters of blood plasma denaturation of a son and a granddaughter, in comparison with the same parameters of a 91 years old grandfather with atherosclerosis. The significant changes in heat (Q(d)) and denaturation temperature (T(d)) of albumin, globulins and fibrinogen of 91-year-old patient suffering from atherosclerosis relative to the norm (88-year-old man) are found out. In particular, Q(d) increase by 60% of albumin fat fraction and fibrinogen of F domain and the increase of their T(d) by 2.

The microcalorimetric investigation of cellular suspensions of blood lymphocytes from healthily and suffering from atherosclerosis individuals.

The denaturation thermodynamic parameters--enthalpy (DeltaH) and temperature of hetero-and active chromatin of lymphocytes and metabolic heat of these cells were determined for healthy and suffering from atherosclerosis individuals. It is supposed that atherosclerosis disease leads to chromatin rebuilding in the interphase state and decrease of cell survival. The chromatin rebuilding may be imagined as partially unfolding of 30 nm chromatin fiber into 10 nm one due to loss of some part of H1 histone.

Microcalorimetric investigation of DNA, poly(dA)poly(dT) and poly[d(A-C)]poly[d(G-T)] melting in the presence of water soluble (meso tetra (4 N oxyethylpyridyl) porphyrin) and its Zn complex.

Thermodynamic parameters of melting process (DeltaHm, Tm, DeltaTm) of calf thymus DNA, poly(dA)poly(dT) and poly(d(A-C)).poly(d(G-T)) were determined in the presence of various concentrations of TOEPyP(4) and its Zn complex. The investigated porphyrins caused serious stabilization of calf thymus DNA and poly poly(dA)poly(dT), but not poly(d(A-C))poly(d(G-T)).

[A microcalorimetric study of collagen hydrated water in a temperature range 20-100 degrees C].

A new method for determining bound water was developed, which is based on precise measurements of the enthalpy of water evaporation from a sample using differential scanning calorimetry.

[Effect of toluene on stability of chromatin of white rat hippocampus and olfactory bulb in vivo. Microcalorimetric study].

The denaturation heat parameters of hippocampus and olfactory bulb nodulus tissues were determined. The total denaturation heat calculated from the areas of endotherms I-IX onto which the dependence deltaH = f(T) is factorized equals to 13.03 +/- 1.

[The influence of the peptide bioregulator prostamax on heterochromatin of human lymphocytes in situ].

It was shown that chromatin contained in human lymphocytes has two stages of denaturation: with T(d)VII = 94.4 degrees C, Q(d)VII = 50.8 J/g DNA, and T(d)VIII = 105.

Thermal characteristics of Spirulina platensis cells under nongrowing conditions at various values of pH medium.

The total value of heat (-Q) evolved by green-blue microalgae Spirulina platensis cells in a dark and stationary regime in the range of pH values 8.0-11.6 was determined.

[Microcalorimetric study of native cells of the microalgae Spirulina platensis in the temperature range (2-55)C].

Thermal effects occurring upon heating a culture of blue-green microalgae Spirulina platensis in the temperature range 5-55 degrees C were studied. Under these conditions, an intensive heat evolution was observed. The heat evolution-versus-temperature curve has a peak with a maximum at approximately 45 degrees C and two distinct shoulders at approximately 25 and 40 degrees C.