Relationship of Inotropic Effects of Stimulation of β- and β-Adrenergic Receptors of Isolated Myocardial Fragments with Echocardiography Parameters in Coronary Heart Disease.

We studied the relationship of inotropic responses of the isolated myocardium to stimulation of β-and β-adrenergic receptors (β-AR) with echocardiography parameters in 28 patients with coronary heart disease (CHD). Myocardial fragments (trabeculae of the right atrial appendage) were obtained during coronary artery bypass surgery. The inotropic response of the trabeculae was assessed in an isometric mode.

Expression of Genes and Proteins of the Sarcoplasmic Reticulum Са-Transport Systems in Cardiomyocytes in Concomitant Coronary Heart Disease and Type 2 Diabetes Mellitus.

We compared the expression of Са-ATPase (SERCA2a), calsequestrin (CASQ2), ryanodine receptors (RyR2) proteins and their genes (ATP2A2, CASQ2, and RYR2) in coronary heart disease (CHD) patients with and without comorbid type 2 diabetes mellitus. All studies were performed on the right atrial appendages resected during coronary bypass surgeries. Expression of SERCA2a and RyR2 proteins and their ATP2A2 (p=0.

[Data on ultrasonographic anatomy of precanal segment of the vertebral artery].

Unlabelled: Described in the article are the results of using ultrasonographic duplex scanning for studying anatomical peculiarities of the precanal segment of the human vertebral artery.

Patients And Methods: Ultrasonographic duplex scanning (USDS) of the extracranial portions of brachiocephalic vessels was performed in a total of 215 inpatients without haemodynamically significant stenoses of the arteries of the vertebrobasilar basin. The patients found to have pathological alterations in the vertebrobasilar basin were excluded from the examined group.

[A new method of endoscopic harvesting of the great saphenous vein in an open system].

We examined a total of 246 patients subjected to coronary artery bypass grafting with the use of the great saphenous vein (GSV). The patients were subdivided into two groups. Group One (n=121) patients endured procurement of the great saphenous vein by a new endoscopic technique in an open system with the help of the equipment Karl Storz and electric dissector Ligasure.

[Changes in Cerebral Blood Flow and Cognitive Function in Patients Undergoing Coronary Bypass Surgery With Cardiopulmonary Bypass].

Purpose: to study effect of coronary artery bypass graft (CABG) surgery with cardiopulmonary bypass (CPB) on regional cerebral blood flow and cognitive function in patients with ischemic heart disease (IHD).

Material And Methods: We included into this study 22 patients with IHD subjected to CABG surgery with CPB. All patients underwent brain SPECT scanning and comprehensive neuropsychological testing 1 day before, 10-14 days and 6 months after surgery.

[Changes in Cerebral Blood Flow and Cognitive Function in Patients Undergoing Coronary Bypass Surgery With Cardiopulmonary Bypass].

Purpose: to study effect of coronary artery bypass graft (CABG) surgery with cardiopulmonary bypass (CPB) on regional cerebral blood flow and cognitive function in patients with ischemic heart disease (IHD).

Material And Methods: We included into this study 22 patients with IHD subjected to CABG surgery with CPB. All patients underwent brain SPECT scanning and comprehensive neuropsychological testing 1 day before, 10-14 days and 6 months after surgery.

[The morphological study of the Perfoost demineralized bone alloimplant applied for the plastic reconstruction of the posterior wall of the external auditory canal].

The objective of the present study was to enhance the effectiveness of the surgical treatment of chronic suppurative otitis media (CSOM) by means of the application of the Perfoost demineralized bone alloimplant for the plastic reconstruction of the posterior wall of the external auditory canal. This aim was attained by the elucidation of morphological features the Perfoost alloimpalnt at the site of surgical intervention one year after the operation. The study included 12 patients presenting with CSOM (epi- and epimesotympanitis) who had underwent "closed-type" sanation surgery of the middle ear.

Cell-free collagen-based scaffolds used for making blood vessels in cardiovascular surgery.

The present article deals with the technology of obtaining decellularized cell-free collagen-based scaffolds from arterial vessels and surgical assessment of the possibility of experimentally implanting them into the blood system of laboratory animals for experimental purposes. The study was performed on arterial vessels (n=60) and fragments of the human internal thoracic artery (n=20). Described herein is a method of obtaining a connective-tissue matrix of a blood vessel by means of vessel's perfusion for 2-3 hours with detergent solutions.

Economical technology of creation of cell-free matrix of animal and human arterial vessels.

We present a technology of creation of blood vessel connective tissue framework by 2-3-h vessel perfusion with detergents. The technology ensures effective removal of vascular cells without damaging collagen and elastic fibers. The connective tissue frameworks prepared by this method can the used for restoring blood flow in various vascular pathologies.

[Scintigraphic evaluation of cerebral circulation in prognosis of cerebrovascular complications of coronary artery bypass grafting].

The aim of the work was to study the possibility of using single-photon emission computed tomography (OECT) of brain using 99m Tc-hexamethyl propylene aminoxime for the evaluation of long-term prognosis of the development of neurocognitive deficit after coronary artery bypass grafting (CBG) and elucidation of relationship between variations of cognitive function and regional cerebral circulation. Surgical myocardial revascularization under artificial circulation was performed in 22 patients with CHD. All of them were examined for the assessment of neurologic and neuropsychological status, and OECT data before, 14 days and 6 months after CBg.

[Clinical experience of the use of autologous mononuclear bone marrow cells in patients with ischemic heart disease and dilated cardiomyopathy].

Between March 2003 and February 2005 cell therapy with mononuclear bone marrow cells (MBMC) was used in 38 and 4 patients with ischemic heart disease and dilated cardiomyopathy, respectively. Intracardiac administration of MBMC (87+/-12 millions) was carried out during coronary artery bypass surgery and/or left ventricular aneurysm resection (n=25) or in cardiac catheterization laboratory (n=17). For verification of cells fixation they were labeled with "Ceretec" 99mTc-HMPAO.

[The long-term results of coronary artery bypass grafting in patients with type II diabetes].

A three-year follow-up of patients with coronary heart disease (CHD) and type II diabetes after coronary artery bypass grafting, shows that the following pathologic conditions are significantly more frequent: arterial hypertension, visceral obesity, marked disturbances of blood lipid spectrum, an increases CHD duration, and an increased rate of myocardial reinfarction and revascularizations. The study shows that the presence of diabetes mellitus in CHD patients undergoing coronary artery bypass grafting, is associated with pronounced disturbances in blood lipid spectrum, and is an important risk factor of coronary event progression.

[Inotropic reactions of myocardium of patients with ischemic heart disease and rats during use of amiodarone].

Aim: To study effect of amiodarone on inotropic reactions of myocardium of patients with ischemic heart disease and rats.

Methods And Results: Excitability of rat myocardium has been found to be higher than that of myocardium of patients. Sarcoplasmic reticulum of myocardium of rats has greater functional reserve, therefore rat muscle preparations respond to extrasystolic stimuli with postextrasystolic potentiation.

Initiation of stress protein synthesis in the myocardium of coronary patients.

We studied myocardial biopsy specimens from the right atrium of cardiological patients with different degree of cardiac ischemia obtained during surgery. No inducible HSP70 stress proteins were detected in atrial cardiomyocytes of patients with the WPW syndrome without signs of ischemic injuries of the heart. These proteins were detected in the myocardium of coronary patients.

Central hemodynamic and cognitive function in the short- and long-term periods after coronary bypass grafting.

The aim of the present work was to study cerebral hemodynamics and cognitive function in patients suffering from coronary artery disease (CAD) in the short- and long-term periods after coronary bypass grafting (CBG). The study accrued 65 patients who underwent myocardial revascularization using cardiopulmonary bypass (43 persons) and without heart interruption (22 persons). In 21 cases, CBG using cardiopulmonary bypass with a purpose of preventing cerebral events was supplemented by administration of instenon manufactured by the Nycomed Company, Perfusion tomoscintigraphy of the brain and neuropsychological testing were provided to all the patients prior to CBG, 14 days and 6 months following surgery.

[Effect of trimetazidine on regressione of ischemic dysfunction of myocardium after coronary bypass surgery on beating heart].

Aim: To study effect of trimetazidine on restoration of hibernating myocardium after myocardial revascularization on beating heart.

Material: Patients with ischemic heart disease subjected to direct myocardial revascularization on beating heart: 25 patients received trimetazidine (60 mg/day) in pre and postoperative periods and 30 patients did not.

Methods: Echocardiography, veloergometry, 6 minute walk test, myocardial scintigraphy with Tl-199.

[A method for preventing recurrent scarring of the tympatic cavity in surgical treatment of patients with suppurative otitis media].

A trial of a novel pathogenetic method to prevent recurrent scarring in surgical treatment of patients with adhesive otitis media was made. The test group consisted of 36 patients with adhesive otitis media in whom surgical treatment was combined with application of mithomycin C (0.2 mg/ml) to the wound area.

[Reperfusion syndrome after direct myocardial revascularization on beating heart in patients with ischemic heart disease. Possibilities of prevention with amlodipine].

Possibilities of secondary prevention of reperfusion syndrome with amlodipine was assessed in 46 patients (39 with class III-IV angina after myocardial infarction and 7 with non ST elevation acute coronary syndrome). Twenty six patients received basic therapy, in 20 patients this therapy was supplemented with amlodipine (5-10 mg/day). In all patients complete direct arterial myocardial revascularization on beating heart was carried out.

[Effect of calcium antagonists verapamil and amlodipine on the risk of development of atrial fibrillation after coronary artery bypass grafting].

Aim: To assess effect of calcium antagonists amlodipine and verapamil on the risk of development of atrial fibrillation after coronary artery bypass surgery.

Material: Of 74 patients subjected to mammary artery and venous coronary bypass grafting with the use of cardiopulmonary bypass 19 received amlodipine and 21 - verapamil.

Results And Conclusion: Attacks of atrial fibrillation during 24 hour ECG monitoring were registered in 22.

[Pharmacological prevention of cerebrovascular complications in cardiac patients after coronary artery bypass surgery].

The aim of the study was to evaluate cognitive functions and cerebral perfusion in cardiac patients following coronary artery bypass graft surgery (CABG) and to investigate a potential of pharmacological prevention of neurocognitive deficit and cerebral perfusion disorders. Forty patients undergone CABG with cardiopulmonary bypass (CPB) were included in the study. For prevention of cerebral perfusion impairments and cognitive deficit, instenon ("Nycomed") was administered to 18 of the patients.

[Prevention of postoperative scars in otorhinolaryngology: experimental rationale].

It is demonstrated that in vitro inhibition of fibroblasts proliferation is feasible with the use of 5-fluorouracil. In stimulated growth of fibroblasts, the antiproliferative effect of 5-fluorouracil manifests even in a minimal concentration (2 mcg/ml). Recovery of fibroblast proliferative activity after elimination of 5-fluorouracil occurs in concentration under 63 mcg/ml.

[Prevention of postoperative scar processes in otorhinolaryngology experimental rationale].

It is shown in vitro that fibroblast proliferation may be inhibited by 5-fluorouracil (5-Fu). In stimulated growth of the fibroblasts the 5-Fu antiproliferative effect takes place even in minimal concentration (2 mcg/ml). Fibroblastic proliferative activity recovers after elimination of 5-Fu from the medium at concentration under 63 mcg/ml.