Mucoadhesive polyelectrolyte complexes of fucoidan and chitin nanowhiskers to prolong the antiprotozoal activity of metronidazole.

The improvement of the specific pharmacological activity of agents with antimicrobial and antiprotozoal properties (e.g. metronidazole, MET) is of interest for clinical applications in the treatment of bacterial infections.

Lactobacilli Have an Opposite Effect on the Resistance to Oxidative Damage of HPV-Infected Compared with Uninfected Vaginal Epithelial Cells.

To study how indigenous or probiotic-introduced lactobacilli affect the sensitivity (estimated as the proportion of surviving, apoptotic, and nonapoptotic deaths) of vaginal epithelial cells obtained from HPV-negative and HPV-positive patients to oxidative damage. The tendency to resist oxidative damage in vaginal epithelial cells of 147 HPV-positive and 59 HPV-negative patients with physiological or suboptimal levels of Lactobacillus was evaluated. Adaptation of cell to curb the oxidative damage in 146 HPV positive and 41 HPV negative with probiotic (Lacticaseibacillus rhamnosus Lcr35) supplementation and without was studied.

Carrageenan/Chitin Nanowhiskers Cryogels for Vaginal Delivery of Metronidazole.

The development of polymeric carriers based on partially deacetylated chitin nanowhiskers (CNWs) and anionic sulfated polysaccharides is an attractive strategy for improved vaginal delivery with modified drug release profiles. This study focuses on the development of metronidazole (MET)-containing cryogels based on carrageenan (CRG) and CNWs. The desired cryogels were obtained by electrostatic interactions between the amino groups of CNWs and the sulfate groups of CRG and by the formation of additional hydrogen bonds, as well as by entanglement of carrageenan macrochains.

Inflammation Mediators Regulate the Microbiota Resistance to Adverse Factors.

We studied the effects of IL-1β, IL-8, TNFα, and prostaglandin E2α in concentrations typically observed in health and during inflammation on the growth of vaginal microbiota and its resistance to factors inhibiting the synthesis of proteins, nucleic acids, and peptidoglycans. An increase in the cytokine levels, characteristic of inflammation, inhibits the growth of Lactobacillus population and improves its resistance to adverse factors. The growth of the population of opportunistic microorganisms (S.

Proinflammatory Cytokines as Regulators of Vaginal Microbiota.

It was shown that IL-1β, IL-8, and IL-6 in concentrations similar to those in the vagina of healthy women stimulated the growth of normal microflora (Lactobacillus spp.) and suppressed the growth and biofilm production by S. aureus and E.

Influence of Hydrogen Peroxide, Lactic Acid, and Surfactants from Vaginal Lactobacilli on the Antibiotic Sensitivity of Opportunistic Bacteria.

We studied as hydrogen peroxide, lactic acid, or surfactants from clinical isolates of vaginal lactobacilli and cell-free supernatants from probiotic strain LCR35 can influence on the sensitivity of opportunistic bacteria to antibiotics. We found that the most effective in increasing sensitivity to antibiotics were hydrogen peroxide and surfactants or their combination but no lactic acid. In some cases, the effect of the composition of hydrogen peroxide and surfactants was clearly higher than the sum of effects of these substances alone.

[EFFECT OF MICROSYMBIONTS ON THE ABILITY OF VAGINAL EPITHELIOCYTES TO MODIFY BIOLOGICAL PROPERTIES OF BACTERIA].

Aim: Study regularities of effects of various types of vaginal microsymbionts on the ability of mucosal epitheliocytes of the vagina to modify biological properties of bacteria.

Materials And Methods: Effect of thermo-inactivated cells of Staphylococcus aureus, Escherichia coli, H2O2-producing and H2O2-non-producing lactobacilli on the ability of primary vaginal epitheliocytes to alter growth and antagonistic activity of Staphylococcus aureus, Escherichia colt, H2O2-producing and H2O2-nonproducing lactobacilli was studied using a multi-component module system.

Results: Alterations of composition of vaginal epitlieliocyte exometabolites under the effect of S.

[The role of pro- and antioxidants of microorganisms in regulation of symbiosis homeostasis mechanisms (on the model of human vaginal biotope)].

Aim: Study the production of bacterial pro- and antioxidants in vaginal biotope and analysis of their role in regulation of symbiosis homeostasis mechanisms.

Materials And Methods: Hydrogen peroxide, catalase inhibitors and antioxidant production in bacteria isolated from 63 women with vaginal eubiosis and 53--with dysbiosis were studied. Production of pro- and antioxidants was regulated by lactate, volatile fatty acids, polyamines and Lactobacillus plantarum and Corynebacterium minutissimum polysaccharides, metabolite bactericidity of peroxide producing lactobacilli was enhanced by addition of iron (II) ions.

[Role of dominant microflora in mechanisms of woman vaginal biotope protection].

Aim: Detection of properties of the dominant microsymbiont emerging in the course of interaction of associative symbiosis components and ensuring protection of vaginal mucosa from seeding by pathogens.

Materials And Methods: H202-producing Lactobacillus spp., Corynebacterium spp.

[Effect of metabolites of H2O2-producing lactobacilli on functional activity of lysozyme].

Aim: Study the effect of metabolites of H2O2-producing lactobacilli on enzymatic and bactericidal activity of lysozyme.

Materials And Methods: 9 H2O2-producing vaginal lactobacilli, Micrococcus luteus NCTC 2665, Escherichia coli State Institute of Standardization and Control No 240367, Lactobacillus acidophilus Institute of Cellular and Intracellular Symbiosis No 37 were used. Ability of lactobacilli to produce H2O2 was evaluated by oxidation of tetramethylbenzidine by peroxidase.

[Role of associative microsymbionts in functioning of associative symbiosis].

Aim: Study the role of associative microsymbionts in biocenosis based on comparative evaluation of interbacterial and epithelial-bacterial interactions on the example of vaginal biotope.

Materials And Methods: Corynebacterium spp., Staphylococcus aureus, Escherichia coli and Lactobacillus spp.

[Influence of corynebacteria metabolites on antagonistic activity of H2O2 producing lactobacilli].

Aim: Study combined influence of Corynebacterium genus bacteria metabolites and H2O2 producing lactobacilli on survival rate of Staphylococcus aureus, Escherichia coli and Lactobacillus acidophilus.

Materials And Methods: The ability to inhibit catalase of the test strains used and to reduce bactericidal effect of hydroxyl radical were determined in corynebacteria. H2O2 containing metabolites were obtained by cultivating lactobacilli in mineral medium, the amount of H2O2 was determined by oxidation of TMB by peroxidase.

[Influence of active forms of oxygen on adhesive characteristics and biofilm production by bacteria].

Aim: Study of influence of low concentrations of active forms of oxygen on the ability of bacteria to form biofilms and their adhesive characteristics.

Materials And Methods: Modification of the studied properties of bacteria was performed by treatment of the bacteria with hydrogen peroxide or hydroxyl radicals at concentrations of 0.5 mM that causes death of 5 - 7% of cells and non-lethal - 0.

[Characteristics of microecological disturbances during endometritis].

Aim: To study the state of vaginal microbiocenosis in women with endometritis and to assess association between microecological disturbances and characteristic of clinical course of the disease.

Materials And Methods: Two hundred women of childbearing age with endometritis were studied, of which 83 had acute form of the disease, and 117 - chronic form. Control group was comprised by 30 clinically healthy women.

[Influence of active forms of oxygen on antilysozyme activity of bacteria].

Aim: To study the effect of active forms of oxygen on bacterial population structure on antilysozyme characteristic.

Materials And Methods: Antilysozyme activity of bacterial clones obtained after their treatment by hydrogen peroxide and hydroxyl radical in sublethal (LD10) and non-lethal concentrations.

Results: Shift of bacterial population structure in direction to increased proportion of clones with high level of antilysozyme activitywith elevating average population level of this characteristic under the influence of sublethal (LD10) concentrations of hydrogen peroxide and hydroxyl radical was established.

[Mechanisms of protective action of bacterial intracellular metabolites for lowering of bactericidal effect of hydroxyl radicals].

Aim: To study mechanisms of attenuation of bactericidal effect of hydroxyl radicals by bacterial intracellular metabolites.

Materials And Methods: Culture liquids of 16 strains of Lactobacillus spp., 21 strains of Corynebacterium spp.

[Active forms of oxygen as a factor regulating surface characteristics of bacterial cells].

Aim: Effects of sublethal concentration of active forms of oxygen (hydrogen peroxide and hydroxyl radical) on cell surface properties of Escherichia coil, Staphylococcus aureus, Bacillus subtilis, and Klebsiella pneumoniae were studied.

Materials And Methods: Hydrophobicity of cell surface measured by transition of cells from water phase to hydrocarbomic phase and by the ability of bacteria to sorb lysozyme and hemoglobin.

Results: Treating of bacterial cells with hydroxyl radicals compared with hydrogen peroxide resulted in more marked decrease of bacteria's hydrophobicity and reduction of their ability to sorb on the surface the lysozyme and hemoglobin.

Bacterial regulation of antagonistic activity of bacteria.

The phenomenon of microbial regulation of bacterial antagonism was studied using metabolites and cell walls of indicator microorganism culture as inductors. The algorithm of selection of stimulators of bacterial antagonistic activity is determined, experimental conditions are described, and methodological approach to stimulation of bacterial antagonistic activity is developed, which can be used for stimulation of probiotic antagonistic activity and for improving colonization resistance of the host organism during infection.

[Mechanisms for survival of bacteria in oxidative stress and iron ions deficiency].

The influence of culture medium Fe2+ content on the resistance of Escherichia coli to hydroxyl radicals formed in the presence of Fe2+ and hydrogen peroxide in Fenton reaction was investigated. It was founded that a lack of Fe2+ in a culture medium increased resistance of bacteria to hydroxyl radicals but not to hydrogen peroxide. The suggestion was made that the lack of Fe2+ starts up synthesis of metabolites which inactivate hydroxyl radical or block Fe2+ ions participating in Fenton reaction.

[The effect of the intra- and extracellular metabolites of microorganisms isolated from various ecotopes on the catalase activity of Staphylococcus aureus 6538P].

The cell extracts (i.e., intracellular metabolites) and culture liquids (i.

[Effect of vaginal Lactobacillus metabolites on biological properties of Staphylococcus epidermidis and Escherichia coli].

The influence of supernatants and cell extracts of vaginal lactic acid bacilli on the growth, catalase, antilysozyme and anticomplementary activity of S. epidermidis and E. coli was studied.

Effects of microbial metabolites on catalase activity and growth of Staphylococcus aureus 6538 P.

The effects of cell extracts and supernatants of Lactobacillus spp. and Corynebacterium spp. on catalase activity and growth of Staphylococcus aureus 6538 P were studied.

[Effect of phytoncides on the dynamics of the cerebral circulation in flight controllers during their occupational activity].

In order to optimize the environment in which flight controllers normally work, volatile phytoncides of brandy mint, lavender, and anise were used. After their 20-day application the health condition of the flight controllers improved. By the end of the working hours they felt less tired.