Publications by authors named "Sezin Erarpat"

This study presents a highly sensitive and accurate analytical strategy for the determination of fenuron in wastewater samples using gas chromatography-mass spectrometry (GC-MS). Simultaneous derivatization and spray-based fine droplet formation-liquid phase microextraction (SFDF-LPME) method was developed and performed to achieve low detection limits. The parameters of the derivatization and SFDF-LPME method were optimized by univariate approach to improve sensitivity and selectivity.

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An accurate and sensitive analytical method was proposed to detect some steroid hormones in biological samples. An FeO/reduced graphene oxide nanocomposite-based dispersive solid-phase extraction was developed for the effective and simple preconcentration of steroid hormones from human serum samples. The nanocomposite was firstly used as adsorbent to simultaneously extract the selected hormones.

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A hyphenated instrumental method high-performance liquid chromatography-inductively coupled plasma-optical emission spectrometry (HPLC-ICP-OES) was used for the separation and determination of Cr(III) and Cr(VI). After the chromatographic separation of chromium species by anion exchange column, their spectrophotometric detection was carried out by ICP-OES system. Important instrumental and chromatographic parameters were investigated via univariate optimization approach to obtain high signal to noise ratio and good resolution for chromium species.

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In this study, an analytical method with high accuracy and precision was developed for the determination of methamphetamine in human urine and serum samples by gas chromatography-mass spectrometry (GC-MS). A simultaneous derivatization and spray assisted droplet formation-liquid phase microextraction (SADF-LPME) method was proposed to derivatize and preconcentrate target analyte. Quadruple isotope dilution (ID) was used to provide high accuracy and precision for methamphetamine determination in the samples.

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A novel nebulizer interface (NI) was proposed to combine high performance liquid chromatography (HPLC) and flame atomic absorption spectrophotometer (FAAS). A glass concentric nebulizer was linked to T-shaped slotted quartz tube (T-SQT) using a tubing to transfer the liquid solution eluted from the chromatographic system into the atomization region of FAAS system. T-SQT was also used to intensify the interaction of atoms with the hollow cathode lamp light.

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Rationale: A derivatization switchable solvent liquid-liquid microextraction quadruple isotope dilution gas chromatography mass spectrometry (D-SS-LLME-ID -GC/MS) method is presented for the determination of hydroxychloroquine sulfate in human biofluids.

Methods: While mixing type/period and concentration of NaOH were optimized via a univariate optimization approach, a multivariate optimization approach was used to determine optimum values for relatively more important parameters such as volumes of derivatization agent (acetic anhydride), NaOH and switchable solvent.

Results: Under the optimum experimental conditions, limit of detection and limit of quantification were calculated as 0.

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The determination of amino acids in biological samples is central to the diagnosis of inherited metabolic disorders and also gives significant information about the metabolisms in the cells and living body. The development of analytical method for reliable quantification of amino acids in biological samples is still challenging because of the polar nature of amino acids and complex nature of biological samples causing a high degree of interferences during analysis. In the present study, a pre-column derivatization method using 2-naphtoyl chloride combined with liquid chromatography-tandem mass spectrometry method was developed for the determination of 17 amino acids in human serum and urine matrices.

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In the present study, a novel analytical method for the determination of hydroxychloroquine sulfate in human serum and urine samples was established. One step derivatization and dispersive liquid-liquid microextraction (DLLME) was developed for quantitative determination of hydroxychloroquine sulfate in aqueous samples. Hydroxychloroquine sulfate was first hydrolyzed and converted to its benzoate derivative by adding benzoyl chloride in chloroform which also served as extraction solvent.

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A new and efficient reversed-phase high-performance liquid chromatography-inductively coupled plasma-optical emission spectrometry method was developed for the simultaneous separation and determination of SeO and seleno-dl-methionine in kefir grains. For the system, limits of detection and quantitation values for SeO and seleno-dl-methionine were calculated as 0.52/1.

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This study presents an accurate and precise analytical strategy for the determination of chloroquine phosphate at trace levels in human body fluids (urine, serum, and saliva). Simultaneous derivatization-spraying based fine droplet formation-liquid phase microextraction (SD-SFDF-LPME) method was used to derivatize and preconcentrate the analyte prior to gas chromatography-mass spectrometry (GC-MS) measurements. Acetic anhydride was employed as derivatizing agent in this study.

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A novel extraction method named hydrogen fluoride assisted-glass surface etching based liquid phase microextraction (HF-GSE-LPME) was proposed to determine 4-n-nonylphenol at trace levels by gas chromatography-mass spectrometry (GC-MS). After the evaluation of system analytical performance for the HF-GSE-LPME-GC-MS system, limit of detection (LOD) and limit of quantification (LOQ) values were calculated as 7.1 and 23.

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Sample preparation methods have become indispensable steps in analytical measurements not only to lower the detection limit but also to eliminate the matrix effect although more sophisticated instruments are being commonly used in routine analyses. Solid phase extraction (SPE) is one of the main extraction/preconcentration methods used to extract and purify target analytes along with simple and rapid procedures but some limitations have led to seek for an easy, sensitive and fast extraction methods with analyte-selective sorbents. Nanoparticles with different modifications have been used as spotlight to enhance extraction efficiency of target pesticides from complicated matrices.

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A rapid, accurate, and sensitive analytical method, ultrasonication-assisted spraying based fine droplet formation-liquid phase microextraction-gas chromatography-mass spectrometry (UA-SFDF-LPME-GC-MS), was proposed for the determination of trace amounts of hydroxychloroquine sulfate in human serum, urine, and saliva samples. To determine the best extraction strategy, several liquid and solid phase extraction methods were investigated for their efficiencies in isolation and preconcentration of hydroxychloroquine sulfate from biological matrices. The UA-SFDF-LPME method was determined to be the best extraction method as it was operationally simple and provided accurate results.

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A vortex assisted spraying based fine droplet formation liquid phase microextraction (VA-SFDF-LPME) method was developed to determine chloroquine phosphate at trace levels in human serum, urine and saliva samples by gas chromatography-mass spectrometry (GC-MS) with single quadrupole mass analyzer. In the first part, several liquid phase microextraction (LPME) and magnetic solid phase extraction (MSPE) methods were compared to each other in order to observe their extraction ability for the analyte. VA-SFDF-LPME method was selected as an efficient and easy extraction method due to its higher extraction efficiency.

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This study proposed a novel analytical method for the separation and determination of cobalamin and cobalt in kefir samples by high performance liquid chromatography-inductively coupled plasma-optical emission spectrometry (HPLC-ICP-OES) in addition to determination of cobalamin in HPLC system. Chromatographic parameters such as column type, buffer solution, mobile phase flow rate and sample injection volume were individually studied and optimized. In addition, cobalamin was simultaneously determined by high performance liquid chromatography with ultraviolet detection (HPLC-UV).

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Article Synopsis
  • A new analytical method for detecting oxcarbazepine in human plasma and urine samples is presented, utilizing a vortex-assisted switchable hydrophilicity solvent-based extraction.
  • The method incorporates optimization of parameters like solvent volume, sodium hydroxide concentration, and vortex timing, achieving a detection range of 27.03 to 353.47 μg/kg.
  • Results show low limits of detection (6.2 μg/kg) and high recovery rates (97-100%) for spiked sample tests, indicating the method's reliability and effectiveness.
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A novel derivatization method for the determination of propineb by gas chromatography-mass spectrometry (GC-MS) was developed in this study. This was achieved by isothiocyanate derivatization of the analyte by potassium persulfate and potassium carbonate in water medium. Dispersive liquid-liquid microextraction (DLLME) was employed to isolate and preconcentrate the derivatized analyte into an organic phase.

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A green and fast analytical method for the determination of l-methionine in human plasma is presented in this study. Preconcentration of the analyte was carried out by switchable solvent liquid phase microextraction after ethyl chloroformate derivatization reaction. Instrumental detection of the analyte was performed by means of gas chromatography-mass spectrometry.

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A switchable solvent based liquid phase microextraction (SS-LPME) has been proposed for the determination of cobalt in egg yolk and Vitamin B12 at trace levels. N,N-Dimethylbenzylamide (DMBA) was used as a switchable solvent and converted to protonated DMBA form by the addition of dry ice. Cobalt was complexed with 1,5-diphenylcarbazone (DPC) and extracted into the DMBA phase.

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Dispersive liquid-liquid microextraction was coupled with quadruple isotope dilution mass spectrometry for the sensitive and accurate determination of parathion methyl in water. The two methods were complementary to each other, with DLLME preconcentrating the analyte for trace determination, and IDMS maintaining the integrity of the method's accuracy and precision. An experimental design was used to optimize the extraction process.

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Polycyclic aromatic hydrocarbons are known for their carcinogenic and mutagenic effects on human health. This therefore calls for the regulation of their concentrations in air, water, and soil. Naphthalene as the simplest in structure of the polycyclic aromatic hydrocarbons is mainly used as a starter material for other chemicals but also has impacts on human health.

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In this study, dispersive liquid-liquid microextraction (DLLME) and slotted quartz tube (SQT) were coupled to flame atomic absorption spectrometry (FAAS) to increase the sensitivity of lead. Conditions such as the formation of the lead-dithizone complex, efficiency of the DLLME method and the output of the SQT were systematically optimized to improve the detection limit for the analyte. The conventional FAAS system was improved upon by about 3.

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