Microbial Antagonism in Food-Enrichment Culture: Inhibition of Shiga Toxin-Producing and Species.

Bacterial pathogens, such as Shiga toxin-producing (STEC) and spp., are important causes of foodborne illness internationally. Recovery of these organisms from foods is critical for food safety investigations to support attribution of illnesses to specific food commodities; however, isolation of bacterial cultures can be challenging.

Neptune: a bioinformatics tool for rapid discovery of genomic variation in bacterial populations.

The ready availability of vast amounts of genomic sequence data has created the need to rethink comparative genomics algorithms using 'big data' approaches. Neptune is an efficient system for rapidly locating differentially abundant genomic content in bacterial populations using an exact k-mer matching strategy, while accommodating k-mer mismatches. Neptune's loci discovery process identifies sequences that are sufficiently common to a group of target sequences and sufficiently absent from non-targets using probabilistic models.

Draft Genome Sequence of Salmonella enterica subsp. enterica Serovar Mishmarhaemek Isolated from Bovine Feces.

Salmonella enterica subsp. enterica serovar Mishmarhaemek is a Gram-negative, non-spore-forming, rod-shaped bacterium implicated in human clinical disease. Here, we report a 4.

The effect of mass, stiffness and geometry on injury outcome in side impacts - a parametric study.

This paper reports on a cooperative research project between the Australian Department of Transport and Regional Services and Transport Canada. This project was a parametric study aimed at better understanding the effects on side impact injury risk of: * Trolley mass * Barrier stiffness * Barrier stiffness distribution * Barrier face height above ground * Crabbed or perpendicular impact * Impact Speed The following observations on injury risk can be made from the tests: * The 2 largest effects for the driver are increasing the height of the barrier face (mainly thoracic) and test speed (all body regions). * Increasing the trolley mass, with a bullet / target mass ratio less than 1, has the effect of increasing only the pubic force.

Concentration and detection of hepatitis A virus and rotavirus in spring water samples by reverse transcription-PCR.

Every year, enteric viruses such as hepatitis A virus (HAV), rotaviruses, and noroviruses are responsible for viral gastro-enteritis and hepatitis reported worldwide. These viruses are mostly transmitted via the faecal-oral route, from direct contact between people, or by ingestion of contaminated food and water. Since only a few viral particles may cause disease, detection of low concentration of these viruses in food matrices is usually complex.

Escherichia coli heat shock protein DnaK: production and consequences in terms of monitoring cooking.

Through use of commercially available DnaK proteins and anti-DnaK monoclonal antibodies, a competitive enzyme-linked immunosorbent assay was developed to quantify this heat shock protein in Escherichia coli ATCC 25922 subjected to various heating regimens. For a given process lethality (F(70)(10) of 1, 3, and 5 min), the intracellular concentration of DnaK in E. coli varied with the heating temperature (50 or 55 degrees C).

Lower limb injuries to passenger car occupants.

A detailed examination was undertaken of hospitalized car occupants who sustained a lower limb injury in a frontal crash. The assessment included an analysis of the type, severity and causes of these injuries and mechanisms involved in lower limb fractures. The findings showed that fractures and dislocations occurred in 88% of lower limb injury cases, that more than half were from crashes < 48 km hour-1 and that the number of fractures was directly proportional with delta V.