Introduction: In addition to the development of analytical equipment, another movement has also appeared in the field of computer assisted techniques for metabolite assessment. Although, some studies can be found in the literature there is still not available reliable and user-friendly software which is coupled with a simple chromatography method for developing a database to identify medicinal plants.
Objectives: Developing a novel similarity search approach for high-performance thin-layer chromatography (HPTLC) fingerprinting.