Sub-minimum inhibitory concentration of tetrakis(hydroxymethyl)phosphonium sulfate enhances biocorrosion of carbon steel by Pseudomonas aeruginosa.

Biocide treatments are commonly employed to mitigate unwanted microbial activities in industrial water systems. This study illuminates the intriguing phenomenon wherein sub-minimum inhibitory concentration (sub-MIC) of tetrakis(hydroxymethyl)phosphonium sulfate (THPS), a frequently used biocide, stimulates the formation of biofilms by Pseudomonas aeruginosa, consequently intensifying the corrosion of carbon steel. Introducing 160 µg/ml THPS, constituting a sub-MIC level, into the culture medium resulted in a notable increase in biofilm thickness and corrosion rate, elevating them from 82 µm and 10 mpy to 97 µm and 18.

Design of a two functional permeable reactive barrier for synergistic enzymatic and microbial bioremediation of phenol-contaminated waters: laboratory column evaluation : Enzymatic and microbial bioremediation of phenol in a bilayer permeable reactive barrier.

The present study aimed to develop a system using a combination of enzymatic and microbial degradation techniques for removing phenol from contaminated water. In our prior research, the HRP enzyme extracted from horseradish roots was utilized within a core-shell microcapsule to reduce phenolic shock, serving as a monolayer column. To complete the phenol removal process, a second column containing degrading microorganisms was added to the last column in this research.

Assessing the efficiency and microbial diversity of HS-removing biotrickling filters at various pH conditions.

This study aimed to investigate the operation of three parallel biotrickling filters (BTFs) in removing HS at different pH conditions (haloalkaliphilic, neutrophilic, and acidophilic) and their associated microbial population in the biodesulfurization process. BTF columns were inoculated with enriched inoculum and experiments were performed by gradually reducing Empty Bed Retention Time (EBRT) and increasing inlet concentration in which the maximum removal efficiency and maximum elimination capacity in EBRT 60 s reached their maximum level in haloalkaline condition (91% and 179.5 g S-HS m h).

Elucidated potential of immobilized Janibacter sp. for saline wastewater phenol removal.

Phenolic compounds are commonly found in industrial effluents and can be hazardous to organisms even at low concentrations. Over the years, researchers have demonstrated that bioremediation is a cost-effective and environmentally friendly alternative to physicochemical approaches used to remove phenol. The aim of this study was to investigate the removal of phenol from saline wastewaters by a halotolerant strain of the genus Janibacter.

Biodegradation of high molecular weight hydrocarbons under saline condition by halotolerant Bacillus subtilis and its mixed cultures with Pseudomonas species.

Biodegradation of high-molecular-weight petroleum hydrocarbons in saline conditions appears to be complicated and requires further investigation. This study used heavy crude oil to enrich petroleum-degrading bacteria from oil-contaminated saline soils. Strain HG 01, with 100% sequence similarity to Bacillus subtilis, grew at a wide range of salinities and degraded 55.

Evaluation of pyrene and tetracosane degradation by mixed-cultures of fungi and bacteria.

The present study was conducted to compare the efficiency of different microbial mixed-cultures consists of fifteen oil-degrading microorganisms with different combinations. The investigation was targeted toward the removal of 500 mg/l pyrene and 1% w/v tetracosane, as single compounds or mixture. Sequential Fungal-Bacterial Mixed-Culture (SMC) in which bacteria added one week after fungi, recorded 60.

Distinct microbial community along the chronic oil pollution continuum of the Persian Gulf converge with oil spill accidents.

The Persian Gulf, hosting ca. 48% of the world's oil reserves, has been chronically exposed to natural oil seepage. Oil spill studies show a shift in microbial community composition in response to oil pollution; however, the influence of chronic oil exposure on the microbial community remains unknown.

Phylogenomics reveals the basis of adaptation of Pseudorhizobium species to extreme environments and supports a taxonomic revision of the genus.

The family Rhizobiaceae includes many genera of soil bacteria, often isolated for their association with plants. Herein, we investigate the genomic diversity of a group of Rhizobium species and unclassified strains isolated from atypical environments, including seawater, rock matrix or polluted soil. Based on whole-genome similarity and core genome phylogeny, we show that this group corresponds to the genus Pseudorhizobium.

The impact of calcium peroxide on groundwater bacterial diversity during naphthalene removal by permeable reactive barrier (PRB).

Oxygen-releasing compounds (ORCs) have recently gained much attention in contaminated groundwater remediation. We investigated the impact of calcium peroxide nanoparticles on the groundwater indigenous bacteria in a bioremediation process by permeable reactive barrier (PRB). Three sand-packed columns were applied, including (1) control column (fresh groundwater), (2) natural remediation column (contaminated groundwater), and (3) biostimulation column (contaminated groundwater amended with CaO).

Bioremediation of benzene-contaminated groundwater by calcium peroxide (CaO) nanoparticles: Continuous-flow and biodiversity studies.

Calcium peroxide (CaO) nanoparticles have been extensively applied in treatment of contaminated groundwater through bioremediation or modified Fenton (MF) processes. In the present study utilization of CaO in bioremediation and MF (CaO+FeSO) reaction is investigated for benzene (50 mg/L) removal in continuous flow sand-packed columns. The results indicated that MF produced OH radicals markedly increased benzene remediation at first 30 days (up to 93%).

Application of encapsulated magnesium peroxide (MgO) nanoparticles in permeable reactive barrier (PRB) for naphthalene and toluene bioremediation from groundwater.

One of the challenges in the petroleum hydrocarbon contaminated groundwater remediation by oxygen releasing compounds (ORCs) is to identify the remediation mechanism and determine the impact of ORCs on the environment and the intrinsic groundwater microorganisms. In this research, the application of encapsulated magnesium peroxide (MgO) nanoparticles in the permeable reactive barrier (PRB) for bioremediation of the groundwater contaminated by toluene and naphthalene was studied in the continuous flow sand-packed plexiglass columns within 50 d experiments. For the biodiversity studies, next generation sequencing (NGS) of the 16S rRNA gene was applied.

Application of magnesium peroxide (MgO) nanoparticles for toluene remediation from groundwater: batch and column studies.

In the present study, magnesium peroxide (MgO) nanoparticles were synthesized by electro-deposition process and characterized by X-ray diffraction (XRD) and field emission scanning electron microscopy (FESEM). The batch experiments were conducted to evaluate the MgO half-life (600 mg/L) in groundwater under various temperatures (4, 15, and 30 °C) and initial pH (3, 7, and 12). The effect of Fe ions (enhanced oxidation) on the toluene remediation by MgO was also investigated.

Evaluation of heavy petroleum degradation using bacterial-fungal mixed cultures.

The use of potent microbial mixed cultures is a promising method for the bioremediation of recalcitrant compounds. In this study, eight molds, three yeasts, and four bacterial isolates were screened from an aged oil-polluted area. An oil degradation assay with various combinations including Bacterial Mixed Culture (BMC), Fungal Mixed Culture (FMC), Fungal-Bacterial Mixed Culture (TMC), and Sequential Fungal-Bacterial Mixed Culture (SMC) was investigated.

Naphthalene remediation form groundwater by calcium peroxide (CaO) nanoparticles in permeable reactive barrier (PRB).

This study investigated the applicability of synthesized calcium peroxide (CaO) nanoparticles for naphthalene bioremediation by permeable reactive barrier (PRB) from groundwater. According to the batch experiments the application of 400 mg/L of CaO nanoparticles was the optimum concentration for naphthalene (20 mg/L) bioremediation. Furthermore, the effect of environmental conditions on the stability of nanoparticles showed the tremendous impacts of the initial pH and temperature on the stability and oxygen releasing potential of CaO.

The ability of Silybum marianum to phytoremediate cadmium and/or diesel oil from the soil.

Phytoremediation is a new ecological and cost-effective technology applied for cleaning heavy metals and total petroleum hydrocarbon contaminated (TPH-contaminated) soils. This study was conducted to evaluate the potential of milk thistle (Silybum marianum) to phytoremediate cadmium (Cd (II)) from contaminated soils. To this end, the investigators applied a completely randomized design with the factorial arrangement and four replications.

Bioremediation of oil-based drill cuttings by a halophilic consortium isolated from oil-contaminated saline soil.

Oil-based drill cuttings are hazardous wastes containing complex hydrocarbons, heavy metals, and brine. Their remediation is a crucial step before release to the environment. In this work, we enriched a halophilic consortium, from oil-polluted saline soil, which is capable of degrading diesel as the main pollutant of oil-based drill cuttings.

Prauserella oleivorans sp. nov., a halophilic and thermotolerant crude-oil-degrading actinobacterium isolated from an oil-contaminated mud pit.

A crude-oil-degrading, Gram-stain-positive actinobacterial strain, RIPIT, was isolated from a soil sample collected from an oil-contaminated mud pit in Khangiran oil and gas field, in the north-east of Iran. RIPIT was strictly aerobic, catalase- and oxidase-positive. The strain grew with 0-12.

Benzene-contaminated groundwater remediation using calcium peroxide nanoparticles: synthesis and process optimization.

Nano-size calcium peroxide (nCaO) is an appropriate oxygen source which can meet the needs of in situ chemical oxidation (ISCO) for contaminant remediation from groundwater. In the present study, an easy to handle procedure for synthesis of CaO nanoparticles has been investigated. Modeling and optimization of synthesis process was performed by application of response surface methodology (RSM) and central composite rotatable design (CCRD) method.

Pseudorhodoplanes sinuspersici gen. nov., sp. nov., isolated from oil-contaminated soil.

Strain RIPI 110T was isolated from a soil sample collected from an oil-contaminated site on Siri Island, Persian Gulf, Iran. Cells of the novel isolate were Gram-stain-negative, facultatively anaerobic, non-motile and rod-shaped. Cells divided asymmetrically by budding and formed rosette-like clusters.

Construction of a horseradish peroxidase resistant toward hydrogen peroxide by saturation mutagenesis.

Horseradish peroxidase (HRP) with a variety of potential biotechnological applications is still isolated from the horseradish root as a mixture of different isoenzymes with different biochemical properties. There is an increasing demand for preparations of high amounts of pure enzyme but its recombinant production is limited because of the lack of glycosylation in Escherichia coli and different glycosylation patterns in yeasts which affects its stability parameters. The goal of this study was to increase the stability of non-glycosylated enzyme, which is produced in E.

Biodegradation of polycyclic aromatic hydrocarbons by a halophilic microbial consortium.

In this study we investigated the phenanthrene degradation by a halophilic consortium obtained from a saline soil sample. This consortium, named Qphe, could efficiently utilize phenanthrene in a wide range of NaCl concentrations, from 1% to 17% (w/v). Since none of the purified isolates could degrade phenanthrene, serial dilutions were performed and resulted in a simple polycyclic aromatic hydrocarbon (PAH)-degrading culture named Qphe-SubIV which was shown to contain one culturable Halomonas strain and one unculturable strain belonging to the genus Marinobacter.

A halotolerant Alcanivorax sp. strain with potential application in saline soil remediation.

Biodegradation of petroleum compounds in saline environments seems intricate and needs more attention. In this study, tetracosane was used to enrich alkane-degrading bacteria from oil-contaminated saline soils. Among the isolates, strain Qtet3, with the highest 16s rRNA gene sequence similarity to Alcanivorax dieselolei B-5(T), was able to grow at a wide range of NaCl concentrations and was shown by GC analysis to degrade more than 90% of tetracosane in 10 days.