Effect of Sodium Alginate-Bulk Chitosan/Chitosan Nanoparticle Wall Matrix on the Viability of Lactobacillus plantarum Under Simulated Gastrointestinal Fluids.

The viability of probiotic cells decreases during passage through the gastrointestinal tract. The process of probiotics encapsulation with sodium alginate and chitosan polymers was carried out to protect the Lactobacillus plantarum in adverse conditions. Lactobacillus plantarum was entrapped in sodium alginate/chitosan (SA/BChi) and sodium alginate/nano-chitosan (SA/NChi) wall materials.

Development of an assay for tetracycline detection based on gold nanocluster synthesis on tetracycline monooxygenase: TetX2@AuNCs.

Gold nanoclusters were synthesized based on the structure of the TetX2 Monooxygenase enzyme to make a nanocluster based on enzyme structure (TetX2@Au-NCs). Kinetic analysis of TetX2@Au-NC nanozyme revealed that the Km values of TetX2@Au-NCs to both HO and TMB chromogenic substrate components are higher in the absence of tetracycline. Additionally, the Vmax of the nanozyme for TMB increased in the presence of tetracycline.

Valorization of Date By-Products: Enhancement of Antioxidant and Antimicrobial Potentials through Fermentation.

The by-products from three varieties of dates-Mozafati, Sayer, and Kabkab-were subjected to solid-state fermentation using alone or in co-culture with or to enhance their phenolic and flavonoid content, along with antioxidant and antimicrobial activities. Solid-state fermentation, being environmentally friendly and cost-effective, is particularly suitable for agricultural residues. Significant increases ( < 0.

New horizon to the world of gut microbiome: seeds germination.

The second brain of humans has been known as the microbiome. The microbiome is a dynamic network composed of commensal bacteria, archaea, viruses, and fungi colonized in the human gastrointestinal tract. They play a vital role in human health by metabolizing components, maturation of the immune system, and taking part in the treatment of various diseases.

Mixed fermentation and electrospray drying for the development of a novel stabilized wheat germ powder containing highly viable probiotic cultures.

Nondairy fermented probiotic powder was developed based on stabilized wheat germ through mixed fermentation ( and ) and electrospraying process. In the first step, the effect of mixed fermentation on lipase and lipoxygenase activity of wheat germ was investigated. The results showed a significant reduction in the activity of both enzymes (82.

Tuning the Physicochemical, Structural, and Antimicrobial Attributes of Whey-Based Poly (L-Lactic Acid) (PLLA) Films by Chitosan Nanoparticles.

Recently, the research and innovation to produce raw materials from microbial processes has gained much attention due to their economic and environmental impacts. Lactic acid is a very important microbial product due to its wide application in the food, pharmaceutical, cosmetic, and chemical industries. In the current study, poly (L-lactic acid) (PLLA) was produced by the ring opening polymerization (ROP) technique of L-lactic acid recovered from whey fermentation, and was used for the production of nanocomposites films reinforced with chitosan nanoparticles (CNPs) (average diameter ca.

Diabetes and seeds: New horizon to promote human nutrition and anti-diabetics compounds in grains by germination.

Type 2 diabetes (T2D) is a complex and heterogeneous chronic metabolic disorder disease that is associated with high blood sugar. Because of the side effects of synthetic drugs on T2D patients and their economic burden, interest in plant-derived functional foods like grains with biological activities has developed. Based on scientific reports, whole grains are rich sources of energy, nutrients, and bioactive compounds and are assumed to have beneficial health effects on glucose enzymes regulation or hyperglycemia.

Economic purification of recombinant uricase by artificial oil bodies.

Rasburicase is an expensive treatment used to control hyperuricemia caused by tumour lysis syndrome (TLS). In this study, a non-chromatographic method was designed based on nano-oil bodies for convenient and economical purification of the recombinant uricase. For this purpose, two chimaeras were synthesized with a different arrangement of the uricase, caleosin and intein fragments.

Canthaxanthin Biofabrication, Loading in Green Phospholipid Vesicles and Evaluation of In Vitro Protection of Cells and Promotion of Their Monolayer Regeneration.

In the present study, canthaxanthin was produced by biofermentation from Dietzia natronolimnaea HS-1 (D. natronolimnaea) and was loaded in phospholipid vesicles prepared with natural component using an easy and low dissipative method. Indeed, glycerosomes, hyalurosomes, and glycerohyalurosomes were prepared by direct hydration of both phosphatidylcholine and the biotechnological canthaxanthin, avoiding the use of organic solvents.

Investigation of the possibility of fermentation of red grape juice and rice flour by and .

The aim of the current study was to evaluate the possibility of the bacterial growth and substrate metabolism during the fermentation of red grape juice and the mixture of red grape juice and rice flour solution using and . In recent years, cereal-based beverages have been used as functional compounds such as antioxidants, dietary fiber, minerals, probiotics, and vitamins in diets. In this research, fermentation of red grape juice (media 1) and 1:1 mixture of red grape juice and rice flour solution (media 2) by two strains of gram positive and homofermentative lactic acid bacteria: and .

Proniosomal Formulation Encapsulating Pomegranate Peel Extract for Nutraceutical Applications.

In this study, pomegranate peel as a traditional natural remedy was extracted and encapsulated in proniosomal systems in order to improve its stability against harsh environmental conditions. Pomegranate peel was extracted by using sonication as a green extraction technology and the antioxidant activity of the obtained extract was evaluated to be 85.37% by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals.

Pulses' germination and fermentation: Two bioprocessing against hypertension by releasing ACE inhibitory peptides.

Angiotensin-Converting Enzyme (ACE) is one of the main blood pressure regulators in the renin-angiotensin system leading to hypertension. Hypertension is known as the modern world disease which increases the risk of serious human health problems. Synthetic drugs and some natural compounds could treat this disease by binding to ACE and reducing its activity.

Green construction of recyclable amino-tannic acid modified magnetic nanoparticles: Application for β-glucosidase immobilization.

The β-glucosidase (BGL) enzyme in food industry is great interest due to its role in food conversion to produce functional food products. In this study, the BGL was covalently immobilized onto amino-tannic acid modified FeO magnetic nanoparticles (ATA-FeO MNPs) as biocompatible nanoplatform by modified poly-aldehyde pullulan (PAP) as a cross-linker to enhance the ability and strength of the nanoparticle connection to the enzyme. The properties of support were subsequently characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier transformed infrared (FTIR), X-ray diffraction (XRD), and vibrating sample magnetometer (VSM).

Microencapsulation of microbial canthaxanthin with alginate and high methoxyl pectin and evaluation the release properties in neutral and acidic condition.

Canthaxanthin (CX) is an orange-red keto-carotenoid with high antioxidant activity. This functional pigment is sensitive to oxygen, light, pH and heat. In this study, CX was produced by the Dietzia natronolimnaea HS-1 and was encapsulated in Alginate (Alg) and Alg-high methoxyl pectin (HMP) through O/W/O multiple emulsion/external gelation method to developed resistant microparticles among acidic and neutral pHs.

Optimization of Effective Minerals on Riboflavin Production by ATCC 6051 Using Statistical Designs.

Background: Riboflavin (vitamin B) is an essential component of the basic metabolism, and an important nutritional and growth factor in humans, animals, plants and micro-organisms. It has been widely used in the fields of pharmaceuticals, feed and food additives. The industrial production of riboflavin mostly relies on the microbial fermentation.

A comparison of Canthaxanthine Pickering emulsions, stabilized with cellulose nanocrystals of different origins.

Cellulosic nanocrystals from different origins were made to stabilize the canthaxanthin (CTX) in pickering emulsion. Nanocrystals were obtained by hydrochloric acid hydrolysis. Dynamic light scattering (DLS) demonstrated that the length of solid particles were in the range of 112nm-4000nm.

Improving the integrity of natural biopolymer films used in food packaging by crosslinking approach: A review.

Currently used approaches for biopolymer modification are either expensive, poisonous or do not lead into the well-desired characteristics to the final film materials. Development of crosslinking procedure is an innovative strategy to improve mechanical, physical and thermal properties of biopolymer films. This review provides a brief description of film-forming biopolymers (e.

Production of Recombinant Antimicrobial Polymeric Protein Beta Casein-E 50-52 and Its Antimicrobial Synergistic Effects Assessment with Thymol.

Accelerating emergence of antimicrobial resistance among food pathogens and consumers' increasing demands for preservative-free foods are two contemporary challenging aspects within the food industry. Antimicrobial packaging and the use of natural preservatives are promising solutions. In the present study, we used -casein-one of the primary self-assembly proteins in milk with a high polymeric film production capability-as a fusion partner for the recombinant expression of E 50-52 antimicrobial peptide in .

Nanoparticles based on crocin loaded chitosan-alginate biopolymers: Antioxidant activities, bioavailability and anticancer properties.

Crocin, a water-soluble carotenoid in saffron, is a highly bioactive compound with limited use due to instability to pH variations, heat and oxidative stress, rapid absorption and low bioavailability. In this study, chitosan-alginate nanoparticles, considered as a new vehicle for crocin to improve properties in terms of antioxidant activity, bioavailability and anticancer activity. The swelling of crocin loaded nanoparticles was pH-dependent so that the highest swelling ratio (16g/g) was observed in acidic condition (pH=1.

Optimization of crosslinked poly(vinyl alcohol) nanocomposite films for mechanical properties.

The effects of glycerol, bacterial cellulose nanocrystal (BCNC) and boric acid concentrations on the mechanical properties of PVA based films, including ultimate tensile strength (UTS), elongation at break (EAB), tensile Young's modulus (TYM), tensile toughness to break (TT), ultimate puncture strength (UPS), puncture deformation (PD), puncture Young's modulus (PYM) and puncture toughness to break (PT), were scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD). Second-order polynomial models with high R values ranging from 0.945 to 0.

Proniosomal powders of natural canthaxanthin: Preparation and characterization.

In this study, canthaxanthin (CTX) was produced by Dietzia natronolimnaea HS-1 using beetroot molasses as substrate and used for encapsulation in proniosome powders after extraction, with the aim of improving its stability. Proniosome powders were prepared with an equimolar ratio of span 60/cholesterol and four different carriers, namely maltodextrin, mannitol, lactose and pullulan. The properties of these formulations as both proniosomal powders and resulted niosomal dispersions were evaluated.

Polysaccharide type and concentration affect nanocomplex formation in associative mixture with β-lactoglobulin.

The main objective of this work was to investigate the effects of polysaccharide type (gum Arabic, GA and/or carboxymethyl cellulose, CMC) and concentration (0-0.2% (w/w)) on the electrostatic interactions with β-lactoglobulin (BLG). Biopolymer interaction was studied using turbidimetric analysis, dynamic light scattering (DLS), electrophoretic mobility and relative viscosity measurements.

Effect of Lactobacillus casei- casei and Lactobacillus reuteri on acrylamide formation in flat bread and Bread roll.

The aim of this study was the evaluation of fermentation by lactic acid bacteria (LAB) contains lactobacillus (L.) casei- casei and L. reuteri on acrylamide formation and physicochemical properties of the Iranian flat bread named, Sangak, and Bread roll.

Recent developments on new formulations based on nutrient-dense ingredients for the production of healthy-functional bread: a review.

Bread is one of the oldest functional foods which its health effects have been investigated in many studies. The current communication presents a review of published studies in recent years on the topic and looks at possible future trends in the improved nutritional and health qualities which have been applied in the bakery industry, directing it further to the formulation design and production of functional breads. The results show that many beneficial ingredients such as dietary fibers, phenolic antioxidants, marine ingredients, and n-3 fatty acids can be used in the bread industry to increase its functionality and result in healthy products, low in calories, cholesterol and celiac disease.

Improvement of crocin stability by biodegradeble nanoparticles of chitosan-alginate.

This study aimed to improve the stability of crocin, a saffron carotenoid, encapsulating into chitosan (Cs)-sodium alginate (Alg) nanoparticles prepared by a modified ionic gelation method were investigated as a new carrier to improve the stability of crocin. Response surface methodology was used to optimize the important variables, namely the concentrations of Cs and Alg, and pH influencing the particle size, zeta-potential, and encapsulation efficiency to find the optimum formulation for production of crocin nanoparticles (CNPs). Microscopic analysis and dynamic light scattering examination indicated non-smooth and spherical nanoparticles with the size range of 165-230 nm in weight ratio of Cs:Alg (1:1.