High-throughput genetic engineering tools for regulating gene expression in a microbial cell factory.

With the rapid advances in biotechnological tools and strategies, microbial cell factory-constructing strategies have been established for the production of value-added compounds. However, optimizing the tradeoff between the biomass, yield, and titer remains a challenge in microbial production. Gene regulation is necessary to optimize and control metabolic fluxes in microorganisms for high-production performance.

Biosensing Applications Using Nanostructure-Based Localized Surface Plasmon Resonance Sensors.

Localized surface plasmon resonance (LSPR)-based biosensors have recently garnered increasing attention due to their potential to allow label-free, portable, low-cost, and real-time monitoring of diverse analytes. Recent developments in this technology have focused on biochemical markers in clinical and environmental settings coupled with advances in nanostructure technology. Therefore, this review focuses on the recent advances in LSPR-based biosensor technology for the detection of diverse chemicals and biomolecules.

Tunable Gene Expression System Independent of Downstream Coding Sequence.

Fine control of the expression levels of proteins constitutes a major challenge in synthetic biology and metabolic engineering. However, the dependence of translation initiation on the downstream coding sequence (CDS) obscures accurate prediction of the protein expression levels from mRNA sequences. Here, we present a tunable gene-expression system comprising 24 expression cassettes that produce predefined relative expression levels of proteins ranging from 0.

Historical record of metal accumulation and lead source in the southeastern coastal region of Korea.

Concentrations of heavy metals and Pb isotopes were measured in the 1-M HCl leaching fraction of core sediments spanning the last 400 years. This sedimentary record of pollution history in metal concentrations shows a good correlation with the increases in industrialization, urbanization, and energy consumption since 1901s. Notably, the Pb concentration and the (207)Pb/(206)Pb and (208)Pb/(206)Pb ratios were constant before the 1910s (16.