Targeting CEA in metastatic triple negative breast cancer with image-guided radiation followed by Fab-mediated chimeric antigen receptor (CAR) T-cell therapy.

Introduction: Although CAR-T cell therapy has limited efficacy against solid tumors, it has been hypothesized that prior treatment with Image-Guided Radiation Therapy (IGRT) would increase CAR-T cell tumor infiltration, leading to improved antigen specific expansion of CAR-T cells.

Methods: To test this hypothesis in a metastatic triple negative breast cancer (TNBC) model, we engineered two anti-CEA single-chain Fab (scFab) CAR-T cells with signaling domains from CD28zeta and 4-1BBzeta, and tested them and .

Results: The anti-CEA scFab CAR-T cells generated from three different human donors demonstrated robust expression, expansion, and lysis of only CEA-positive TNBC cells, with the CD28z-CAR-T cells showing the highest cytotoxicity.

Predictors of vaccine uptake during a pandemic: The interplay of lifetime discrimination, educational attainment, and family support.

The experience of discrimination can have significant health implications, especially during a global pandemic. This study examines how lifetime discrimination, educational attainment (measured in years of education), and family support individually and interactively predict COVID-19 vaccine uptake. Low educational attainment may amplify the impact of discrimination due to increased vulnerability to misinformation.

Essentializing Happiness Mitigates the Changes in Subjective Well-Being Following Negative Life Events.

People hold different beliefs about the changeability of happiness. Some believe that happiness is biologically predetermined and thus unchangeable (essentialist beliefs), while others believe that it is malleable and can be changed (non-essentialist beliefs). Do these beliefs have a tangible impact on how individuals actually experience well-being? Here, we predict and empirically demonstrate that endorsing essentialist beliefs about happiness (EBH) can result in a self-fulfilling prophecy that buffers the changes in subjective well-being (SWB) following life events.

Post COVID-19, still wear a face mask? Self-perceived facial attractiveness reduces mask-wearing intention.

With the emerging post-COVID era, wearing face masks has become a domain of personal choice. Then, who wants to continue wearing a mask when it is no longer mandatory? In this article, we expect and examine the role of self-perceived facial attractiveness in predicting mask-wearing intention and its mechanism across three studies (total  = 1,030). Studies 1 and 2 demonstrated that individuals with high (vs.

"Why rush to get vaccinated earlier?": Regulatory focus and COVID-19 vaccination.

Objectives: The health psychology literature has paid little attention to individuals' intention to vaccinate . Building on regulatory focus theory, which proposes two distinct self-regulatory foci: promotion and prevention focus (Higgins, 1997), the present study tests whether, how, and why regulatory focus contributes to the intention to vaccinate earlier (IVE) and whether IVE predicts actual behavior.

Method: This study used a longitudinal design with data collected at two-time points (3.

Tumor regression and immunity in combination therapy with anti-CEA chimeric antigen receptor T cells and anti-CEA-IL2 immunocytokine.

Targeted immunotherapy of solid cancers with chimeric antigen receptor (CAR) T cells and immunocytokines are attractive options in that they both rely on the specificity of tumor-targeted antibodies. Since carcinoembryonic antigen (CEA) expression in both colon and breast cancers is correlated with poor prognosis, it was chosen as a model tumor target in immunocompetent CEA transgenic (CEATg) mice. A second-generation anti-CEA CAR derived from CEA-specific antibody T84.

Preparation and characterization of gatifloxacin-loaded alginate/poly (vinyl alcohol) electrospun nanofibers.

The aim of this study was to develop novel biomedicated electrospun nanofibers for controlled release. Pre-formulation studies were carried out for nanofibers of sodium alginate (SA) (2 wt %)/polyvinyl alcohol (PVA) (10 wt %) composites (2/8, 3/7 and 4/6), by an electrospinning technique. The morphology and average diameter of the nanofibers were investigated by scanning electron microscopy (SEM).

Identification of a functional proprotein convertase cleavage site in microfibril-associated glycoprotein 2.

Microfibril-associated glycoprotein 2 (MAGP2) is a secreted protein associated with multiple cellular activities including the organization of elastic fibers in the extracellular matrix (ECM), angiogenesis, as well as regulating Notch and integrin signaling. Importantly, increases in MAGP2 positively correlate with poor prognosis for some ovarian cancers. It has been assumed that full-length MAGP2 is responsible for all reported effects; however, here we show MAGP2 is a substrate for the proprotein convertase (PC) family of endoproteases.