Transcriptomic insights into the antagonistic responses of Antarctic marbled rockcod, Notothenia rossii, to elevated temperature and acidification.

The escalating impacts of climate change, particularly ocean acidification and warming, are pivotal stressors for marine ecosystems and have profound effects on biota in polar regions. This study investigated the immunological responses of the Antarctic fish Notothenia rossii to environmental stressors indicative of future ocean conditions under the Intergovernmental Panel on Climate Change Shared Socioeconomic Pathways 5-8.5 scenario for 2100.

Genomic profiling of Antarctic geothermal microbiomes using long-read, Hi-C, and single-cell techniques.

Geothermal features in Antarctica provide favorable conditions for diverse microorganisms, yet their genomic diversity remains poorly understood. Here, we present an integrated dataset comprising PacBio HiFi and Hi-C metagenomic sequencing, along with single-cell amplified genomes (SAGs) from two high-altitude geothermal sites, Mount Melbourne and Mount Rittmann, in Antarctica. The long-read HiFi sequencing, coupled with Hi-C, enhances the understanding of microbiome diversity and functionality in this unique ecosystem by providing more complete and accurate genomic information.

Wearable-Based Integrated System for In-Home Monitoring and Analysis of Nocturnal Enuresis.

Nocturnal enuresis (NE) is involuntary bedwetting during sleep, typically appearing in young children. Despite the potential benefits of the long-term home monitoring of NE patients for research and treatment enhancement, this area remains underexplored. To address this, we propose NEcare, an in-home monitoring system that utilizes wearable devices and machine learning techniques.

Structural and Biochemical Insights into Bis(2-hydroxyethyl) Terephthalate Degrading Carboxylesterase Isolated from Psychrotrophic Bacterium .

This study aimed to elucidate the crystal structure and biochemically characterize the carboxylesterase Est2, a thermotolerant biocatalyst derived from , a psychrotrophic bacterium. Sequence and phylogenetic analyses showed that Est2 belongs to the Family XIII group of carboxylesterases. Est2 has a broad range of substrate specificities for short-chain -nitrophenyl (NP) esters, 1-naphthyl acetate (1-NA), and 1-naphthyl butyrate (1-NB).

Feruloyl Esterase (Fae) from : Structural Insights and Functional Characterization for Application in Ferulic Acid Production.

Ferulic acid and related hydroxycinnamic acids, used as antioxidants and preservatives in the food, cosmetic, pharmaceutical and biotechnology industries, are among the most abundant phenolic compounds present in plant biomass. Identification of novel compounds that can produce ferulic acid and hydroxycinnamic acids, that are safe and can be mass-produced, is critical for the sustainability of these industries. In this study, we aimed to obtain and characterize a feruloyl esterase (Fae) from .

Improved natural food colorant production in the filamentous fungus Monascus ruber using CRISPR-based engineering.

Monascus pigments have various food industry applications and are pharmacologically active. Genome sequencing-based clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology has been implemented to increase pigment production in Monascus. To increase pigment production in M.

Structural and biochemical insights into PsEst3, a new GHSR-type esterase obtained from Paenibacillus sp. R4.

PsEst3, a psychrophilic esterase obtained from Paenibacillus sp. R4, which was isolated from the permafrost of Alaska, exhibits relatively high activity at low temperatures. Here, crystal structures of PsEst3 complexed with various ligands were generated and studied at atomic resolution, and biochemical studies were performed to analyze the structure-function relationship of PsEst3.

Crystal structure and biochemical analysis of acetylesterase (LgEstI) from Lactococcus garvieae.

Esterase, a member of the serine hydrolase family, catalyzes the cleavage and formation of ester bonds with high regio- and stereospecificity, making them attractive biocatalysts for the synthesis of optically pure molecules. In this study, we performed an in-depth biochemical and structural characterization of a novel microbial acetylesterase, LgEstI, from the bacterial fish pathogen Lactococcus garvieae. The dimeric LgEstI displayed substrate preference for the short acyl chain of p-nitrophenyl esters and exhibited increased activity with F207A mutation.

Gene family expansions in Antarctic winged midge as a strategy for adaptation to cold environments.

Parochlus steinenii is the only flying insect native to Antarctica. To elucidate the molecular mechanisms underlying its adaptation to cold environments, we conducted comparative genomic analyses of P. steinenii and closely related lineages.

Safety evaluation of mycotoxin citrinin production from through whole-genome sequencing and analytical evaluation.

In this study, the whole genome of KACC 46666 was generated using the PacBio RSII sequencer with high-quality de novo assembly to obtain trustworthy assembly and annotation using genome assemblies with long reads from PacBio single-molecule real-time sequencing. The whole genome of has a total length of 25.9 Mb, divided in 13 contigs with 9639 genes.

Investigating the Functional Role of the Cysteine Residue in Dehydrin from the Arctic Mouse-Ear Chickweed .

The stress-responsive, SK subclass, dehydrin gene, , has been identified from the Arctic mouse-ear chickweed . CaDHN contains an unusual single cysteine residue (Cys143), which can form intermolecular disulfide bonds. Mutational analysis and a redox experiment confirmed that the dimerization of CaDHN was the result of an intermolecular disulfide bond between the cysteine residues.

Bioactive Terphenyls Isolated from the Antarctic Lichen .

Three -terphenyls (-)-2-hydroxy-3,5-dimethoxy--terphenyl (), 2-hydroxy-3,6-dimethoxy--terphenyl (), and 2,3,5,6-tetramethoxy--terphenyl ()-were isolated for the first time as natural products along with seven known compounds (, -) from the Antarctic lichen Structures of the new compounds were elucidated by comprehensive analyses of 1D and 2D NMR and HREIMS experiments. Compound exhibited cytotoxicity against HCT116 cells with the IC value of 3.76 ± 0.

The complete chloroplast genome sequence of a medicinal citrus landrace, Hort. ex Tanaka in Jeju Island, Korea.

(Dongjeongkyool in Korean) is a medicinal citrus landrace that grows in Korea. In this study, we characterized the complete chloroplast (Cp) genome (160,120 bp) of . The Cp genome was consisted of 4 distinct regions: a large single copy (87,731 bp), a small single copy (18,393 bp), and a pair of inverted repeat regions (26,998 bp).

sp. nov., isolated from a marine sediment of the East Siberian Sea and sp. nov., isolated from an Antarctic lichen.

Gram-stain-negative, strictly aerobic, red-pink-coloured, rod-shaped and non-motile bacterial strains PAMC 29290, PAMC 29294 and PAMC 29296 were isolated from marine surface sediment sampled in the East Siberian Sea and strains PAMC 26553 and PAMC 26554 were obtained from an Antarctic lichen. Strains PAMC 29290, PAMC 29294 and PAMC 29296 were closely related to (98.8 % 16S rRNA gene similarity), (97.

Complete genome sequence of Rhodoferax sp. PAMC 29310 from a marine sediment of the East Siberian Sea.

Rhodoferax sp. PAMC 29310 was isolated from a surface marine sediment of the East Siberian Sea, Arctic. Whole-genome sequencing of the strain Rhodoferax sp.

CRISPR/Cas9-mediated Inactivation of arginase in a yeast strain isolated from Nuruk and its impact on the whole genome.

Despite the advantages of CRISPR/Cas9 technology in the food industry, controversy over its off-target effects exists. We engineered an industrial Saccharomyces cerevisiae strain isolated from a Korean rice wine starter, Nuruk, using CRISPR/Cas9 to decrease ethyl carbamate (EC) formation. We disrupted the CAR1 gene encoding arginase, which plays a key role in EC formation.

Crystal structure of a MarR family protein from the psychrophilic bacterium sp. TG-14 in complex with a lipid-like molecule.

MarR family proteins regulate the transcription of multiple antibiotic-resistance genes and are widely found in bacteria and archaea. Recently, a new MarR family gene was identified by genome analysis of the psychrophilic bacterium sp. TG-14, which was isolated from sediment-laden basal ice in Antarctica.

Disproportionate microbial responses to decadal drainage on a Siberian floodplain.

Permafrost thaw induces soil hydrological changes which in turn affects carbon cycle processes in the Arctic terrestrial ecosystems. However, hydrological impacts of thawing permafrost on microbial processes and greenhouse gas (GHG) dynamics are poorly understood. This study examined changes in microbial communities using gene and genome-centric metagenomics on an Arctic floodplain subject to decadal drainage, and linked them to CO and CH flux and soil chemistry.

Mitochondrial genome of the Antarctic microalga KSF0127 (Chlorellaceae, Trebouxiophyceae).

We report the first mitochondrial genome of the Antarctic microalga KSF0127. The circular mitochondrial genome was 67,923 bp in length and contained 45 protein-coding genes, one ribosomal RNA gene, and 60 transfer RNA genes. The phylogenetic tree was constructed with eight previously reported mitogenome sequences and showed the phylogenetic position of KSF0127 within the Chlorellaceae family.

Screening and Genetic Network Analysis of Genes Involved in Freezing and Thawing Resistance in -Expressing Using Gene Expression Profiling.

The cryoprotection of cell activity is a key determinant in frozen-dough technology. Although several factors that contribute to freezing tolerance have been reported, the mechanism underlying the manner in which yeast cells respond to freezing and thawing (FT) stress is not well established. Therefore, the present study demonstrated the relationship between encoding monodehydroascorbate reductase from Antarctic hairgrass and stress tolerance to repeated FT cycles (FT2) in transgenic yeast .

Complete chloroplast genome sequence of a medicinal landrace citrus Jinkyool ( Hort. ex Tanaka) in Jeju Island, Korea.

(Jinkyool) is a medicinal landrace citrus belonging to the Rutaceae family. We determined the complete chloroplast genome (160,699 bp) of CRS0085 in Jeju Island, Korea. The genome is composed of four distinct parts; a large single copy of 87,918 bp, a small single copy of 21,355 bp, and a pair of inverted repeat regions of 25,713 bp.

Continuous body impedance measurement to detect bladder volume changes during urodynamic study: A prospective study in pediatric patients.

Aims: To assess the possibility of the body impedance (BI) reflecting bladder volumes (BV) in pediatric patients, the BI signals are measured continuously with the equipment that we have developed and reported previously, during the filling phase of urodynamic study (UDS).

Methods: A total of 30 children (5-12 years old) are included in this prospective study. The equipment uses two dry electrodes embedded inside a strap to collect impedance and electrocardiogram signals.