Iron-Chelating Hydroxyketone Ligands Promote Degradation of Fe(III)-Tannic Acid Nanofilms.

Polyphenols form nanofilms with transition metal ions by coordination-driven assembly. The as-formed metal-polyphenol nanofilms can degrade in the presence of chelating ligands that exhibit high stability constant with the nanofilm-forming metal ions. We have demonstrated the degradation of Fe(III)-tannic acid nanofilms with hydroxyketone ligands, such as maltol, kojic acid, and deferiprone, which exhibit high availability and excellent cytocompatibility.

Valorization of Cabbage Waste as a Feedstock for Microbial Polyhydroxyalkanoate Production: Optimizing Hydrolysis Conditions and Polyhydroxyalkanoate Production.

Establishing a platform for the bioconversion of waste resources into value-added compounds is critical for achieving a sustainable and eco-friendly economy. Herein, we produced polyhydroxyalkanoate via microbial fermentation using cabbage waste as a feedstock and metabolically engineered . For this, the hydrolysis conditions of cabbage waste were optimized by focusing on parameters such as substrate and enzyme concentrations to enhance the saccharification efficiency.

Utilization of coffee waste as a sustainable feedstock for high-yield lactic acid production through microbial fermentation.

Lactic acid is an important industrial precursor; however, high substrate costs are a major challenge in microbial fermentation-based lactic acid production. Coffee waste is a sustainable feedstock alternative for lactic acid production via microbial fermentation. Herein, the feasibility of coffee waste as a feedstock was explored by employing appropriate pretreatment methods and optimizing enzyme combinations.

Heterotypic stress-induced adaptive evolution enhances freeze-drying tolerance and storage stability of Leuconostoc mesenteroides WiKim33.

Lactic acid bacteria (LAB) are currently being investigated for their potential use as probiotics and starter cultures. Researchers have developed powdering processes for the commercialization of LAB. Previous studies have focused on identifying innovative cryoprotective agents and freeze-drying (FD) techniques to enhance the stability of LAB.

Rapid analysis of insecticidal metabolites from the entomopathogenic fungus Beauveria bassiana 331R using UPLC-Q-Orbitrap MS.

Beauveria bassiana, a representative entomopathogenic fungus, is increasingly being utilized as an eco-friendly pest management alternative to chemical insecticides. This fungus produces a range of insecticidal secondary metabolites that act as antimicrobial and immunosuppressive agents. However, detailed qualitative and quantitative analysis related to these compounds remains scarce, we developed a method for the rapid analysis of these metabolites.

Sonochemical application reduces monosaccharide levels and improves cryoprotective effect of Jerusalem artichoke extract on Leuconostoc mesenteroides WiKim33 during freeze-drying.

Lactic acid bacteria (LAB) are being used for probiotic and starter cultures to prevent global damage to microbial cells. To retain the benefits of LAB in the commercially used powdered form, highly efficient cryoprotective agents are required during the manufacturing process. This study suggests a novel cryoprotective agent derived from Jerusalem artichoke (JA; Helianthus tuberous L.

Root-Knot Nematode () Control Using a Combination of WiKim0090 and Copper Sulfate.

Lactic acid bacteria (LAB) exert antagonistic activity against root-knot nematodes, mainly by producing organic acids via carbohydrate fermentation. However, they have not yet been used for root-knot nematode () control owing to a lack of economic feasibility and effectiveness. In this study, we aimed to isolate organic acid-producing LAB from kimchi (Korean traditional fermented cabbage) and evaluated their nematicidal activity.

Resveratrol-β-Lactoglobulin Composite Nanocoating by Layer-by-Layer Assembly with Fe(III)-Tannic Acid Complex.

Resveratrol (3,4',5-trihydroxystilbene) is beneficial to human health due to its diverse biological activities including its anti-inflammatory and anti-oxidative effects as confirmed by pharmacokinetic tests. Despite these clinical merits, resveratrol's limited hydrosolubility and chemical vulnerability remain challenging with regard to developing a controlled delivery system with enhanced bioavailability. In this work, we report a resveratrol-β-lactoglobulin (R-BLG) composite nanocoating through a layer-by-layer assembly with Fe(III)-tannic acid nanofilms.

Draft Genome Sequence of EL000614, a Strain Producing Grammicin, a Potent Nematicidal Compound.

An endolichenic fungus, strain EL000614, showed strong nematicidal effects against plant pathogenic nematode, by producing grammicin. We report genome assembly of EL000614 comprised of 25 scaffolds with a total length of 54.73 Mb, N50 of 4.

Chemically Robust Antifog Nanocoating through Multilayer Deposition of Silica Composite Nanofilms.

A coating must remain intact to perform its inherent functions on a surface, and often functional organic coatings fail due to deterioration because of their intrinsic vulnerabilities. In this work, we present a biomimetic material based on a glass sponge to provide a robust silica composite nanocoating with an antifog effect. The silica composite nanocoating was constructed with a binary film structure consisting of (1) a Fe(III)-tannic acid (TA) nanofilm for adhesion to coat the substrates and (2) a SiO layer to enhance the durability of the coating.

Nematicidal activity of 5-iodoindole against root-knot nematodes.

Multi-drug resistance in nematodes is a serious problem as lately several resistant phenotypes have emerged following the intermittent usage of synthetic nematicides. Contemporary research continues to focus on developing and/or repurposing small molecule inhibitors that are eco-friendly. Here, we describe the repurposing of the indole derivative, 5-iodoindole, as a nematicide for the root-knot nematode, Meloidogyne incognita.

Biological Control of Root-Knot Nematodes by Organic Acid-Producing WiKim0069 Isolated from Kimchi.

Root-knot nematodes (RKNs) are among the most destructive plant-parasites worldwide, and RKN control has been attempted mainly using chemical nematicides. However, these chemical nematicides have negative effects on humans and the environment, thus necessitating the search for eco-friendly alternative RKN control methods. Here, we screened nematicidal lactic acid bacteria (LAB) isolated from kimchi and evaluated their efficacy as biocontrol agents against RKNs.

Thickness-Tunable Eggshell Membrane Hydrolysate Nanocoating with Enhanced Cytocompatibility and Neurite Outgrowth.

The eggshell membrane is one of the easily obtainable natural biomaterials, but has been neglected in the biomaterial community, compared with marine biomaterials and discarded as a food waste. In this work, we utilized the ESM hydrolysate (ESMH), which was obtained by the enzymochemical method, as a bioactive functional material for interfacial bioengineering, exemplified by thickness-tunable, layer-by-layer (LbL) nanocoating with the Fe(III)-tannic acid (TA) complex. [Fe(III)-TA/ESMH] LbL films, ending with the ESMH layer, showed great cytocompatiblility with HeLa cells and even primary hippocampal neuron cells.

Conformationally Locked Tolans, β-Sheet Structures, and Photophysical Properties.

Conformationally locked tetrasubstituted tolans were synthesized by introducing a tether on the tolan. To demonstrate the utilities of these motifs, a β-hairpin structure (15) was synthesized, and its additional stabilizing effects were evaluated. Moreover, the photophysical properties of cyclic tolans and their β-sheet structure were investigated.

Hg(II)-mediated intramolecular cyclization reaction in aqueous media and its application as Hg(II) selective indicator.

The Hg(II)-specific intramolecular cyclization reaction of ethynyl phenols was carried out for the first time in semiaqueous media at ambient temperature. The reaction unit (ethynyl phenol) was coupled with a malononitrile derivative (signal unit), which afforded the chromogenic Hg(II) indicator 7. The reaction of the chromogenic Hg(II) indicator 7 was further optimized in DMSO/water (3:7, v/v) (10 mM PBS buffer, pH = 7.