Freezing of human immature oocytes using cryoloops with Taxol in the vitrification solution.

Purpose: In human frozen immature oocytes, there has been little successful delivery. We examined the feasibility of vitrification solution including Taxol, cytoskeltal stabilizer.

Methods: We set four experimental groups that immature oocytes has cumulus cells or not, or including Taxol or not in the vitrification solution.

Physical and mental development of children after fertilization and embryo transfer.

To evaluate the physical and mental development of children after fertilization (IVF) and frozen embryo transfer (FET). Between July 1995 and November 2003, 506 patients delivered 658 babies after IVF and ET treatment at our clinic (intracytoplasmic sperm injection (ICSI), 418; conventional IVF, (C-IVF) 125; FET, 115). A survey of the physical and mental developmental of the children was conducted by mailing questionnaires to parents.

Two-step consecutive transfer of early embryos and blastocysts.

To evaluate and confirm the merit of two consecutive transfer attempts of early embryos and blastocysts. A total of 685 patients underwent routine fertilization (IVF) cycles. The study population consisted of three groups: (i) Group 1, early stage embryos were transferred (460 patients; 567 cycles); (ii) group 2, blastocysts were transferred (88 patients; 105 cycles); and (iii) group 3, a two-step (consecutive) transfer was performed on 137 women (141 cycles).

Successful delivery following cryopreservation of zygotes produced by in vitro matured oocytes retrieved from a woman with polycystic ovarian syndrome-like disease: a case report.

Purpose: To estimate frozen zygotes, which developed from in vitro matured oocytes retrieved from polycystic ovarian syndrome-like disease.

Methods: Oocyte retrieval was performed on Day 15 following withdrawal bleeding. The oocytes were incubated for 24 h in TCM-199 maturation medium supplemented with follicle fluid, E2, FSH, and hCG.