CRISPR/Cas9 Gene Knockout in Medullary Thyroid Carcinoma Cell-lines: Optimization and Validation.

Background: Medullary Thyroid Cancer (MTC) is a very aggressive type of thyroid carcinoma. Mutation in proto-oncogene is demonstrated in MTC development. We aimed to knock-out of -oncogene using CRISPR/Cas9 genome editing method in MTC cell-lines.