Polyacrylamide-based semi-interpenetrating networks for entrapment of laccase and their use in azo dye decolorization.

In this study, laccase from Trametes versicolor was immobilized in poly(acrylamide-crotonic acid)/sodium alginate [P(AAm-CrA)/alginate], poly(acrylamide-crotonic acid)/K-carrageenan [(P(AAm-CrA)/K-car)], poly(acrylamide-citraconic acid)/sodium alginate (P(AAm-CA)/alginate), and poly(acrylamide-citraconic acid)/K-carrageenan (P(AAm-CA)/K-car) semi-interpenetrating network by entrapment method. Optimum pH and temperatures values were determined between 5.0-6.

Synthesis and surface modification of polyurethanes with chitosan for antibacterial properties.

Surface modification and providing antibacterial properties to the materials or devices are getting great attention especially in the last decades. In this study, polyurethane (PU) films were prepared by synthesizing them in medical purity from toluene diisocyanate and polypropylene ethylene glycol without using any other ingredients and then the film surfaces were modified by covalent immobilization of chitosan (CH) which has antibacterial activity. CH immobilized PU films (PU-CH) were found to be more hydrophilic than control PU films.

Immobilization of laccase in kappa-carrageenan based semi-interpenetrating polymer networks.

Laccase enzyme (L) was immobilized by entrapment into semi-interpenetrating polymer networks (semi-IPNs) prepared from kappa-carrageenan with either poly(acrylamide-acrylic acid) [P(AAm-AA)/kappa-car] or poly(acrylamide-itaconic acid) [P(AAm-IA)/kappa-car]. For both systems, immobilized enzymes achieved the same optimum values observed for free enzyme (pH=5.0 and T=40 degrees C), except for P(AAm-IA)/kappa-car system there was a shift to 5.