Effects of Elevated Temperature on Nodule Development: I-Detailed Characteristic of Unusual Apical Senescence.

Despite global warming, the influence of heat on symbiotic nodules is scarcely studied. In this study, the effects of heat stress on the functioning of nodules formed by bv. strain 3841 on pea () line SGE were analyzed.

Effects of Elevated Temperature on Nodule Development: II-Phytohormonal Responses.

High temperature is one of the most important factors limiting legume productivity. We have previously shown the induction of senescence in the apical part of nodules of the pea SGE line, formed by bv. strain 3841, when they were exposed to elevated temperature (28 °C).

Efficacy of a Plant-Microbe System: (L.) Cadmium-Tolerant Mutant and Strains, Expressing Pea Metallothionein Genes and , for Cadmium Phytoremediation.

Two transgenic strains of bv. , 3841-PsMT1 and 3841-PsMT2, were obtained. These strains contain the genetic constructions and coding for two pea ( L.

Gibberellins Inhibit Nodule Senescence and Stimulate Nodule Meristem Bifurcation in Pea ( L.).

The development of nitrogen-fixing nodules formed during -legume symbiosis is strongly controlled by phytohormones. In this study, we investigated the effect of gibberellins (GAs) on senescence of pea () symbiotic nodules. Pea wild-type line SGE, as well as corresponding mutant lines SGEFix-1 (), SGEFix-2 (), SGEFix-3 (), and SGEFix-7 (), blocked at different stages of nodule development, were used in the study.

Early nodule senescence is activated in symbiotic mutants of pea (Pisum sativum L.) forming ineffective nodules blocked at different nodule developmental stages.

Plant symbiotic mutants are useful tool to uncover the molecular-genetic mechanisms of nodule senescence. The pea (Pisum sativum L.) mutants SGEFix-1 (sym40), SGEFix-3 (sym26), and SGEFix-7 (sym27) display an early nodule senescence phenotype, whereas the mutant SGEFix-2 (sym33) does not show premature degradation of symbiotic structures, but its nodules show an enhanced immune response.

Analysis of nodule senescence in pea (Pisum sativum L.) using laser microdissection, real-time PCR, and ACC immunolocalization.

A delay in the senescence of symbiotic nodules could prolong active nitrogen fixation, resulting in improved crop yield and a reduced need for chemical fertilizers. The molecular genetic mechanisms underlying nodule senescence have not been extensively studied with a view to breeding varieties with delayed nodule senescence. In such studies, plant mutants with the phenotype of premature degradation of symbiotic structures are useful models to elucidate the genetic basis of nodule senescence.

[The role of immunodeficiencies in development of complications during vaccination of children with BCG vaccine].

Aim: Study of the interrelation between the presence of immune deficiency and development of complications during vaccination of newborns with BCG vaccine.

Materials And Methods: In 24 children with complications of vaccine process in the form of cold abscess and lymphadenitis indicators of lymphocyte subpopulation levels were studied by flow cytofluorimetry on Beckman Coulter cytofluoriemter by using monoclonal antibodies with markers CD45+CD3+ - T-cell, CD45+CD3+CD4+ - T-helpers, CD45+CD3+CD8+ - T-supressors-cytotoxic killers, CD45+CD3 CD16+CD56+ - natural killers, CD45+CD3-CD19+ - B-lymphocytes. The level of IgG, IgA, IgM in sera was determined by immune diffusion method in agar by Mancini.

[Cytokine profile during experimental gastric and duodenal ulcers: the serotonin role].

We investigated the levels of proinflammatory and antiinflammatory cytokines in experimental gastric and duodenal ulcers. Precedence shown an increase of pro-inflammatory cytokine TNF-a level in the isolated simulation of gastric ulcers and duodenal ulcers and preferential increase in cytokine IL-4 level in a prior administration of serotonin. The development of chronic diseases of the gastrointestinal tract is largely mediated by immunological reactions and the activation of the serotonergic system.

[Autoimmune reactions in diseases of the digestive system organs].

Aim: To examine content, diagnostic and prognostic role of autologous antibodies in gastrointestinal diseases (GID).

Material And Methods: Enzyme immunoassay was used to measure content of autologous antibodies to N+/K+ ATPase (Aab) of gastric parietal cells, mitochondria, microsomes, tissue transglutaminase in blood serum of 196 patients with gastric, gallbladder, small and large intestinal diseases. Aab relations with heterologous antibodies were studied with kits provided by Bektor-Best (Novosibirsk), DRG-Diagnostics, Orgentec (Germany) and others.

[Diagnostic and prognostic value of humoral immune status indices for alimentary system diseases].

The paper describes the results of studying the immune status of 1,960 patients with stomach, pancreas, liver, gall bladder, small and large intestine disorders, who were treated in the Central Research Institute of Gastroenterology. The results of the study demonstrate that alimentary system diseases are concomitant with changes in the functional activity of the immune system and development of the systemic immune response aimed at the neutralization and elimination of pathogenic agents. Impaired regulatory and efferent lymphocyte capacities, increased synthesis of cytokines, immunoglobulins, heterologous (anti-viral, anti-bacterial or antigliadin), autologous (to parietal cells, microsome mitochondria, tissue transglutaminase) antibodies, formation of immune complexes, autoimmune reactions and secondary immunodeficiency are specific immune mechanisms of the pathological process development, its synchronization and progression in patients with alimentary system diseases.

[A cytokine status in chronic alcoholic and biliary pancreatitis].

Aim: To determine characteristics of a cytokine status in chronic pancreatitis (CP) depending on etiological factor, stage of the disease, complications, therapy. Material and methods. 72 patients had chronic alcoholic pancreatitis (CAP), 38 patients--chronic biliary pancreatitis (CBP).

[Antibodies to Helicobacter pylori in gastric diseases].

Aim: To study the count of antibodies to Helicobacter pylori (ABHP), their role in diagnosis and prognosis in gastric diseases (GD).

Material And Methods: Enzyme immunoassay (kits from EcoLab and Vektor-Best, Russia) was used to measure serum levels of ABHP (IgG, IgA, IgM+IgG+IgA) in 46 GD patients.

Results: It is shown that GD are characterized by a regular rise of serum ABHP which are serological markers of Helicobacter pylori infection.

[Anti-Helicobacter pylori antibodies in cases of stomach polyps].

Unlabelled: The goal of the study was to study the content of anti-Helicobacter pylori antibodies (at-HP), their diagnostic and prognostic significance for stomach polyps.

Materials And Methods: The content of anti-Helicobacter antibodies (IgG-HP; IgA-HP; IgM + IgG + IgA-CagA-HP) was determined in the blood serum by way of immune-enzyme analysis of 46 patients with stomach polyps. Such test systems as EcoLab (Elektrostal) and Vector-Best (Novosibirsk) were used.

[Current understanding of primary biliary cirrhosis].

Aim: To examine characteristics of the course of primary biliary cirrhosis (PBC).

Material And Methods: The study of 150 PBC patients aged 26-82 years used clinical, biochemical, immunological and morphological methods.

Results: Antimitochondrial antibodies of type M2 (AMAM2) were detected in 85.

[Cytokines and cytokine therapy in gastrointestinal diseases].

Aim: To compare cytokine status in gastrointestinal diseases (GID) with reference to etiological factor, course, stage, therapy of the disease.

Material And Methods: Enzyme immunoassay was used to examine cytokines in the peripheral blood, tissue homogenates of 560 GID patients. GID were represented by ulcer disease (UD), cholelithiasis, chronic hepatitis (CH), glutenic enteropathy (GE), Crohn's disease (CD), nonspecific ulcer colitis (NUC).