Breast cancer recurrence is associated with the growth of disseminated cancer cells that separate from the primary tumor before surgical treatment and hormonal therapy and form a metastatic niche in distant organs. We previously demonstrated that IGFBP6 expression is associated with the risk of early relapse of luminal breast cancer. Knockdown of IGFBP6 in MDA-MB-231 breast cancer cells increased their invasiveness, proliferation, and metastatic potential.
View Article and Find Full Text PDFIntroduction: The new coronavirus disease, COVID-19, poses complex challenges exacerbated by several factors, with respiratory tissue lesions being notably significant among them. Consequently, there is a pressing need to identify informative biological markers that can indicate the severity of the disease. Several studies have highlighted the involvement of proteins such as APOA1, XPNPEP2, ORP150, CUBN, HCII, and CREB3L3 in these respiratory tissue lesions.
View Article and Find Full Text PDFWe propose and demonstrate dendrimer-based coatings for a sensitive biochip surface that enhance the high-performance sorption of small molecules (i.e., biomolecules with low molecular weights) and the sensitivity of a label-free, real-time photonic crystal surface mode (PC SM) biosensor.
View Article and Find Full Text PDFProtein nanoparticles (NPs) can be used as vaccine platforms for target antigen presentation. To conduct a proof-of-concept study to demonstrate that an effective NP platform can be built based on a short self-assembling peptide (SAP) rather than a large self-assembling protein. SUMO-based protein fusions (SFs) containing an N-terminal SAP and a C-terminal antigen were designed, expressed in and purified.
View Article and Find Full Text PDFZh Nevrol Psikhiatr Im S S Korsakova
January 2013
A metallocarboxypeptidase produced by Streptomyces bikiniensis 27 strain (VKPM Ac-1783) (CPSb) was purified and characterized. The enzyme cleaves both basic and hydrophobic C-terminal amino acid residues from synthetic peptides, that is, it possesses specificity of mammalian carboxypeptidases A and B. The enzyme also hydrolyzes peptides bearing glutamic acid at the C-end.
View Article and Find Full Text PDFZh Nevrol Psikhiatr Im S S Korsakova
December 2010
Intellectual, psychomotor and speech development of 50 children born to mothers with epilepsy were studied. The sample has been stratified into 2 subgroups: children aged from 6 months to 4 years and children aged from 4 to 16 years. This group included 4 (8%) children with children absence epilepsy, juvenile myoclonic and unclassified epilepsy; one patient had a diagnosis of epileptic encephalopathy without epileptic seizures.
View Article and Find Full Text PDFThe gene phyA encoding phytase was isolated from Obesumbacterium proteus genomic library and sequenced. The cleavage site of the PhyA signal peptide was predicted and experimentally proved. The PhyA protein shows maximum identity of 53% and 47% to phosphoanhydride phosphorylase from Yersinia pestis and phytase AppA from Escherichia coli, respectively.
View Article and Find Full Text PDFThe data on the precursors of bacterial proteases were generalized. The structure and special features of processing of the precursors of bacillary subtilisins, the alpha-lytic protease from Lysobacter enzymogenes and the related chymotrypsin-like proteases from Streptomyces griseus, and the metalloproteases from bacilli and Pseudomonas aeruginosa were discussed. The approaches to producing the precursors and the protease propeptides and to in vitro characterizing them were particularly analyzed.
View Article and Find Full Text PDFFunctional destination of propeptides and precursors in bacillar secretory proteases remains uncertain. Formerly deletion assay demonstrated folding and secretion of subtilisin E, chymotrypsin-like protease SGPB from S. griseus and B.
View Article and Find Full Text PDFA secretory system based on L-form cells of Proteus mirabilis was developed for production of native Bacillus licheniformis glutamylendopeptidase precursor never formerly available. The produced precursor was stable per se under physiological conditions and in presence of trypsin and glutamylendopeptidase from B. intermedius.
View Article and Find Full Text PDFThe pLF1311 natural plasmid from Lactobacillus fermentum 1311 was used to construct a single-replicon vector suitable for rapid cloning in a wide range of gram-positive hosts and Escherichia coli. The new vector is capable of conjugative mobilization from E. coli to various hosts by conjugal transfer.
View Article and Find Full Text PDFA 15-bp mini-gene was introduced into Bacillus subtilis and into stable protoplast-like L-forms of Proteus mirabilis. This mini-gene encoded the peptide MVLFV and modeled a fragment of Escherichia coli 23S rRNA responsible for E. coli erythromycin (Ery) resistance.
View Article and Find Full Text PDFA metalloprotease gene of Brevibacillus brevis (npr) was expressed in Escherichia coli in a soluble form as native Npr precursor. A significant fraction of the precursor was spontaneously processed, producing the N-terminal propeptide and the mature enzyme. A strong inhibition of the mature Npr by its own propeptide in the crude lysate was observed even in the absence of the covalent linkage between them.
View Article and Find Full Text PDFThe structural gene of the carboxypeptidase T (cpt) was successfully expressed in cell wall-less L-form cells of Proteus mirabilis. The DNA sequence encoding the PhoA leader peptide was fused with a truncated cpt gene encoding the mature enzyme. The modified gene in a pUC-based kanamycin resistance vector under the control of the lac promoter was transformed into L-form cells of P.
View Article and Find Full Text PDFExperimental data are discussed and experience in complex treatment of 102 patients with acute pancreatitis with the application of intralesional+ electrophoresis is analysed. It was demonstrated in experiments on 90 albino rats that in intralesional+ electrophoresis of radioactive++ 5-fluorouracil (5-FU), its concentration in the pancreas was 2.4-3-fold that in the group of animals in which only intraperitoneal injections of the agent were given.
View Article and Find Full Text PDFA total of 1545 patients, referred with the diagnosis of obliterating endarteritis to the angiologic unit of the Irkutsk Vascular Centre from various other medical institutions, are examined. Differential diagnosis of the ischemic lower limb syndrome, rooted in vascular and other diseases, is shown to be possible, using noninvasive diagnostic procedures on an outpatient basis. The diagnosis of obliterating endarteritis was only verified in 18.
View Article and Find Full Text PDFKardiologiia
December 1980
Analysis is made of 1,014 cases with occlusive lesion of the bifurcation of the aorta and iliofemoral segment in patients with chronic ischemic syndrome of the lower limbs. The cause of high arterial occlusion with atherosclerosis in 95.5% of patients and nonspecific aortitis in 12 cases.
View Article and Find Full Text PDFKhirurgiia (Mosk)
February 1971