Expression of a periplasmic β-glucosidase from Yarrowia lipolytica allows efficient cellobiose-xylose co-fermentation by industrial xylose-fermenting Saccharomyces cerevisiae strains.

This study aimed to compare the effects of cellobiose hydrolysis, whether occurring inside or outside the cell, on the ability of Saccharomyces cerevisiae strains to ferment this sugar and then apply the most effective strategy to industrial S. cerevisiae strains. Firstly, two recombinant laboratory S.

Production and concentration of keratinases and application of fermentation residual in removing hexavalent chromium.

The production of keratinases was evaluated in submerged fermentation with Aspergillus niger and by pigs' swine hair in a batch bioreactor. Experimental planning was performed to assess the interaction between different variables. The enzyme extract produced was characterized at various pH and temperatures and subjected to enzyme concentration using a biphasic aqueous system and salt/solvent precipitation techniques.

Trichoderma koningiopsis fermentation in airlift bioreactor for bioherbicide production.

During scaling of fermentations, choosing a bioreactor is fundamental to ensure the product's quality. This study aims to produce bioherbicides using Trichoderma koningiopsis fermentation, evaluating process parameters in an Airlift bioreactor. As a response, we quantified the production of enzymes involved in the bioherbicide activity (amylase, cellulase, laccase, lipase, and peroxidase).

Biochemical and Biotechnological Insights into Fungus-Plant Interactions for Enhanced Sustainable Agricultural and Industrial Processes.

The literature is full of studies reporting environmental and health issues related to using traditional pesticides in food production and storage. Fortunately, alternatives have arisen in the last few decades, showing that organic agriculture is possible and economically feasible. And in this scenario, fungi may be helpful.

Challenges and opportunities for third-generation ethanol production: A critical review.

In recent decades, third-generation (3G) biofuels have become a more attractive method of fuel production, as algae cultivation does not infringe on resources needed for food production. Additionally, algae can adapt to different environments, has high photosynthetic efficiency (CO fixation), and has a high potential for carbohydrate accumulation. The prevalence of algae worldwide demonstrates its ability to adapt to different environments and climates, proving its biodiversity and versatility.

Analysis of glucose and xylose metabolism in new indigenous Meyerozyma caribbica strains isolated from corn residues.

Aiming to broaden the base of knowledge about wild yeasts, four new indigenous strains were isolated from corn residues, and phylogenetic-tree assemblings on ITS and LSU regions indicated they belong to Meyerozyma caribbica. Yeasts were cultivated under full- and micro-aerobiosis, starting with low or high cell-density inoculum, in synthetic medium or corn hydrolysate containing glucose and/or xylose. Cells were able to assimilate both monosaccharides, albeit by different metabolic routes (fermentative or respiratory).

Production of biofuels from soybean straw and hull hydrolysates obtained by subcritical water hydrolysis.

The objective of this study was to evaluate the ethanol production by Wickerhamomyces sp. using soybean straw and hull hydrolysates obtained by subcritical water hydrolysis and, afterward, the biogas production using the fermented hydrolysates. Ethanol was produced using the straw and hull hydrolysates diluted and supplement with glucose, reaching 5.

Biochemical analysis of cellobiose catabolism in Candida pseudointermedia strains isolated from rotten wood.

We isolated two Candida pseudointermedia strains from the Atlantic rain forest in Brazil, and analyzed cellobiose metabolization in their cells. After growth in cellobiose medium, both strains had high intracellular β-glucosidase activity [~ 200 U (g cells) for 200 mM cellobiose and ~ 100 U (g cells) for 2 mM pNPβG] and negligible periplasmic cellobiase activity. During batch fermentation, the strain with the best performance consumed all the available cellobiose in the first 18 h of the assay, producing 2.

Advances in yeast alcoholic fermentations for the production of bioethanol, beer and wine.

Yeasts have a long-standing relationship with humankind that has widened in recent years to encompass production of diverse foods, beverages, fuels and medicines. Here, key advances in the field of yeast fermentation applied to alcohol production, which represents the predominant product of industrial biotechnology, will be presented. More specifically, we have selected industries focused in producing bioethanol, beer and wine.

Bioethanol Production From Hydrolyzed Lignocellulosic After Detoxification Via Adsorption With Activated Carbon and Dried Air Stripping.

Bioethanol production has been presented as an alternative for supplying energy demand and minimizing greenhouse gases effects. However, due to abrasively conditions employed on the biomass during pretreatment and hydrolysis processes, inhibitors for fermentation phase such as acetic acid and others can be generated. Based on this problem, the aim of this work was to evaluate the adsorption of acetic acid on microporous activated carbon and investigate the stripping of the same component with dried air.

Effect of pretreatments on corn stalk chemical properties for biogas production purposes.

Different pretreatments were evaluated on corn stalk (Zea mays) applied as a lignocellulosic source in anaerobic co-digestion with swine manure, using sulfuric acid (HSO) and hydrogen peroxide (HO) for biogas production purposes. Using HSO we achieved a 75.1% removal of the hemicellulose fraction, in low acid concentrations (0.

Second-generation ethanol from non-detoxified sugarcane hydrolysate by a rotting wood isolated yeast strain.

This work aims to evaluate the production of second-generation ethanol from sugarcane bagasse hydrolysate without acetic acid (inhibitor) detoxification. Three isolated yeast strains from lignocellulosic materials were evaluated, and one strain (UFFS-CE-3.1.

Draft Genome Sequence of the D-Xylose-Fermenting Yeast Spathaspora arborariae UFMG-HM19.1AT.

The draft genome sequence of the yeast Spathaspora arborariae UFMG-HM19.1A(T) (CBS 11463 = NRRL Y-48658) is presented here. The sequenced genome size is 12.

Industrial fuel ethanol yeasts contain adaptive copy number changes in genes involved in vitamin B1 and B6 biosynthesis.

Fuel ethanol is now a global energy commodity that is competitive with gasoline. Using microarray-based comparative genome hybridization (aCGH), we have determined gene copy number variations (CNVs) common to five industrially important fuel ethanol Saccharomyces cerevisiae strains responsible for the production of billions of gallons of fuel ethanol per year from sugarcane. These strains have significant amplifications of the telomeric SNO and SNZ genes, which are involved in the biosynthesis of vitamins B6 (pyridoxine) and B1 (thiamin).

Switching the mode of sucrose utilization by Saccharomyces cerevisiae.

Background: Overflow metabolism is an undesirable characteristic of aerobic cultures of Saccharomyces cerevisiae during biomass-directed processes. It results from elevated sugar consumption rates that cause a high substrate conversion to ethanol and other bi-products, severely affecting cell physiology, bioprocess performance, and biomass yields. Fed-batch culture, where sucrose consumption rates are controlled by the external addition of sugar aiming at its low concentrations in the fermentor, is the classical bioprocessing alternative to prevent sugar fermentation by yeasts.

Molecular analysis of maltotriose active transport and fermentation by Saccharomyces cerevisiae reveals a determinant role for the AGT1 permease.

Incomplete and/or sluggish maltotriose fermentation causes both quality and economic problems in the ale-brewing industry. Although it has been proposed previously that the sugar uptake must be responsible for these undesirable phenotypes, there have been conflicting reports on whether all the known alpha-glucoside transporters in Saccharomyces cerevisiae (MALx1, AGT1, and MPH2 and MPH3 transporters) allow efficient maltotriose utilization by yeast cells. We characterized the kinetics of yeast cell growth, sugar consumption, and ethanol production during maltose or maltotriose utilization by several S.