Magnetic Resonance Imaging in Situ Visualization of an Electrochemical Reaction under Forced Hydrodynamic Conditions.

Magnetic resonance imaging (MRI) has proven to be a powerful tool for the characterization and investigation of in situ chemical reactions. This is more relevant when dealing with complex systems, where the spatial distribution of the species, partition equilibrium, flow patterns, among other factors have a determining effect over mass transport and therefore over the reaction rate. The advantage of MRI is that it provides spatial information in a noninvasive way and does not require any molecular sensor or sample extraction.

Binding of the highly toxic tetracycline derivative, anhydrotetracycline, to bovine serum albumin.

Tetracycline (TC) derivatives are extensively used as antibiotics in human and animal medicine and, very recently, they have been screened as anti-amyloidogenic drugs. Anhydrotetracycline (AHTC) is one of the major degradation products of TC that has been linked to several side effects of the drug. We evaluated the interaction of AHTC with bovine serum albumin (BSA), one of the main carriers of amphiphilic molecules in blood, using three complementary analytical methods: fluorescence spectroscopy, isothermal titration calorimetry and differential scanning calorimetry.