Degradation of C-Steroids and Effect of Androstenedione on Gene Expression in Nocardioides simplex.

Molecular mechanisms of C-steroid core degradation have been intensively studied mostly in mycolic acid rich actinobacteria, mainly in the representatives of Mycobacterium, Mycolicibacterium and Rhodococcus genera, whilst much less data evidencing functionality of the 9(10)-seco pathway in other actinobacteria was reported. In this study, degradation of androstenedione (AD), androstadienedione (ADD), testosterone (T), Δ-dehydrotestosterone (DT) and 9α-hydroxyandrostenedione (9α-OH-AD) by the biotechnologically relevant actinobacterium of Nocardioides simplex VKM Ac-2033D was investigated. Key intermediates of 9α-OH-AD degradation were isolated and identified as 3-hydroxy-9,10-seco-androsta-1,3,5(10)-triene-9,17-dione (3-HSA) and 3,17β-dihydroxy-9,10-seco-androsta-1,3,5(10)-trien-9-one (3,17-DHSA).

Draft genome sequence of glyphosate-degrading sp. strain GPK 3 (VKM B-2554D) isolated from agricultural soil.

The genome of sp. isolated from agricultural soil polluted with the herbicide glyphosate is reported. The genome size is 5.

Deep dive into the diversity and properties of rhodopsins in actinomycetes of the family Geodermatophilaceae.

In recent decades, most studies of microbial rhodopsins have focused on their identification and characterization in aquatic bacteria. In 2021, actinomycetes of the family Geodermatophilaceae, commonly inhabiting terrestrial ecosystems in hot and arid regions, have been reported to contain rhodopsins with DTEW, DTEF and NDQ amino acid motifs. An advanced bioinformatics analysis performed in this work additionally revealed NTQ rhodopsin and heliorhodopsins.

Proposal of Crystallibacter gen. nov., Crystallibacter permensis sp. nov. and Crystallibacter degradans sp. nov. for the salt-tolerant and aromatics degrading actinobacteria, and reclassification of Arthrobacter crystallopoietes as Crystallibacter crystallifaciens comb. nov.

Four salt-tolerant and aromatics degrading strains used in this study were isolated from polluted technogenic soil on the territory of the Verkhnekamsk potash deposit (Russia). The strains were aerobic, Gram-stain-positive, non-motile, non-endospore-forming irregular rods, exhibiting a marked rod-coccus growth cycle. They contained lysine-based peptidoglycan, teichulosonic acid and poly(glycosyl phosphate) polymers in the cell walls.

Glyphosate-Induced Phosphonatase Operons in Soil Bacteria of the Genus .

and , bacterial degraders of the herbicide glyphosate, were found to induce phosphonatase (phosphonoacetaldehyde hydrolase, EC 3.11.1.

Rathayibacter tanaceti sp. nov., a Novel Actinobacterium from Tanacetum vulgare Infested by Foliar Nematode Aphelenchoides sp.

Two novel yellow-pigmented, rod-shaped and non-motile coryneform actinobacteria, strains VKM Ac-2596 and VKM Ac-2761, were isolated from a plant Tanacetum vulgare (Asteraceae) infested by foliar nematode Aphelenchoides sp. The strains exhibited the highest 16S rRNA gene sequence similarities to Rathayibacter agropyri CA4 (99.71%), Rathayibacter rathayi DSM 7485 (99.

Identification of a Gene Encoding a New Fungal Steroid 7-Hydroxylase and Its Functional Characterization in Yeast.

The hydroxylation of steroids in the C7β position is one of the rare reactions that allow the production of value-added precursors in the synthesis of ursodeoxycholic acid and other pharmaceuticals. Recently, we discovered this activity in the ascomycete sp. VKM F-3040.

transcriptome assembly of sp. VKM F-3040, a promising steroid-modifying ascomycete.

This research presents transcriptome shotgun assembly for sp. VKM F-3040, which is a putative fungal strain able to modify androstane steroids with production of 7-hydroxy and 17-hydroxylated derivatives-key intermediates in the synthesis of pharmaceutical ingredients. The data are of importance for creating novel microbial biocatalysts.

Transcriptomic Analysis Followed by the Isolation of Extracellular Bacteriolytic Proteases from VKM B-2533.

The aim of the study was to search for, isolate and characterize new bacteriolytic enzymes that show promising potential for their use in medicine, agriculture and veterinary. Using a transcriptomic analysis, we annotated in VKM B-2533 the genes of known bacteriolytic and antifungal enzymes, as well as of antibiotics, whose expression levels increased when cultivated on media conducive to the production of antimicrobial agents. The genes of the secreted putative bacteriolytic proteases were also annotated.

Ancylobacter radicis sp. nov., a novel aerobic methylotrophic bacteria associated with plants.

The two novel bacterial strains, designated as VT and ML, were isolated from roots of cinquefoil (Potentilla sp.) and leaves of meadow-grass (Poa sp.) on the flooded bank of lake, respectively.

Draft Genome Sequences of 11 Rhodopsin Gene-Containing Actinobacteria () from Saline Arid Habitats in the Central Asian Deserts.

Draft genome sequences of 11 strains of putative new species of were generated using Illumina technology. The genome sizes ranged from 4.19 to 4.

Distribution of Bacterial Endosymbionts of the Clade in Plant-Parasitic Nematodes.

Bacteria of the genus " Cardinium" and related organisms composing the clade are intracellular endosymbionts frequently occurring in several arthropod groups, freshwater mussels and plant-parasitic nematodes. Phylogenetic analyses based on two gene sequences (16S rRNA and ) showed that the clade comprised at least five groups: A, B, C, D and E. In this study, a screening of 142 samples of plant-parasitic nematodes belonging to 93 species from 12 families and two orders using PCR with specific primers and sequencing, revealed bacteria of clade in 14 nematode samples belonging to 12 species of cyst nematodes of the family Heteroderidae.

Insight into Different Stages of Steroid Degradation in Thermophilic VKM Ac-666 Strain.

Steroids are abundant molecules in nature, and various microorganisms evolved to utilize steroids. Thermophilic actinobacteria play an important role in such processes. However, very few thermophiles have so far been reported capable of degrading or modifying natural sterols.

Draft Genome Sequences of 9 Actinobacteria from the Family Associated with Insect- and Nematode-Damaged Plants.

Draft genome sequences of 9 strains of known and putative new species of isolated from insect- and nematode-damaged plants were generated using Illumina technology. The data obtained will contribute to the development of the genome-based prokaryote taxonomy and the knowledge on the biology of the microbial group investigated.

Expression of a Stilbene Synthase Gene from the x L. Hybrid Increases the Resistance of Transgenic L. Plants to .

'Isabel' grape ( x L. hybrid) is one of the main grape cultivars in Russia and some other countries for processing, due to its vigor, tolerance to the main fungal diseases, high yield and potential for sugar accumulation. The stilbene synthase gene was isolated from the hybrid grape cv.

Steroid Metabolism in Thermophilic Actinobacterium VKM Ac-666.

The application of thermophilic microorganisms opens new prospects in steroid biotechnology, but little is known to date on steroid catabolism by thermophilic strains. The thermophilic strain VKM Ac-666 has been shown to convert various steroids and to fully degrade cholesterol. Cholest-4-en-3-one, cholesta-1,4-dien-3-one, 26-hydroxycholest-4-en-3-one, 3-oxo-cholest-4-en-26-oic acid, 3-oxo-cholesta-1,4-dien-26-oic acid, 26-hydroxycholesterol, 3β-hydroxy-cholest-5-en-26-oic acid were identified as intermediates in cholesterol oxidation.

Draft Genome Sequences of 28 Actinobacteria of the Family Associated with Nematode-Infected Plants.

Draft genome sequences of 28 strains of from plants infested by plant-parasitic nematodes were obtained using Illumina technology. The sequence data will provide useful baseline information for the development of comparative genomics and systematics of and facilitate understanding of molecular mechanisms involved in interactions between plants and nematode-associated bacterial complexes.

Draft Genome Sequence of the Type Strain Lysobacter capsici VKM B-2533.

VKM B-2533 is a promising strain for isolation of new lytic agents. Here, we report a draft genome sequence of this strain, consisting of 131 scaffolds with a total length of 6,196,943 bp. The results obtained will aid in the discovery and study of biologically active compounds important for biomedicine.

Draft Genome Sequences of 13 Plant-Associated Actinobacteria of the Family .

Draft genome sequences of 13 bacterial strains from the family were generated using Illumina technology. The genome sizes varied from 3.0 to 4.

Comparative Genomics of Strains D-401 and D-402 with Contrasting Physiology but Extremely High Level of Genomic Identity.

Representatives of filamentous colorless sulfur-oxidizing bacteria often dominate in sulfide biotopes, preventing the diffusion of toxic sulfide into the water column. One of the most intriguing groups is a recently described including strains D-401 and D-402. Both strains have identical genes encoding enzymes which are involved in the oxidation of hydrogen sulfide and thiosulfate.

Complete and Draft Genome Sequences of 12 Plant-Associated Strains of Known and Putative New Species.

Complete and draft genome sequences of 12 strains were generated using Oxford Nanopore and Illumina technologies. The genome sizes of these strains are 3.21 to 4.

Draft Genome Sequence of Glyphosate-Degrading Achromobacter insolitus Strain Kg 19 (VKM B-3295), Isolated from Agricultural Soil.

The genome of an strain isolated from an agricultural soil polluted with the herbicide glyphosate is reported. The genome size is 6.4 Mb, with an average G+C content of 65.